{"text_format":"1","workbook":"xlsx","n_sheets":37,"sheets":["Read me first","PSPT scheme Opt1","Step1_Input OD450 data","Step2_Curvefit_plate 1","Step2_Curvefit_plate 2","Step2_Curvefit_plate 3","Step2_Curvefit_plate 4","Step2_Curvefit_plate 5","Step2_Curvefit_plate 6","Step2_Curvefit_plate 7","Step2_Curvefit_plate 8","Step2_Curvefit_plate 9","Step2_Curvefit_plate 10","Step2_Curvefit_plate 11","Step2_Curvefit_plate 12","Step2_Curvefit_plate 13","Step2_Curvefit_plate 14","Step2_Curvefit_plate 15","Step2_Curvefit_plate 16","Step2_Curvefit_plate 17","Step2_Curvefit_plate 18","Step2_Curvefit_plate 19","Step2_Curvefit_plate 20","Step2_Curvefit_plate 21","Step2_Curvefit_plate 22","Step2_Curvefit_plate 23","Step2_Curvefit_plate 24","Step2_Curvefit_plate 25","Step2_Curvefit_plate 26","Step2_Curvefit_plate 27","Step2_Curvefit_plate 28","Step2_Curvefit_plate 29","Step2_Curvefit_plate 30","Step2_Curvefit_plate 31","Step2_Curvefit_plate 32","Step 3_IgG titers","Summary IgG titers"],"not_rendered":{"charts":32,"images":1,"macros":1,"external_links":0,"defined_names":866,"comments":32,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Read me first","index":0,"n_rows":59,"n_cols":8,"rows_kept":59,"cut":false,"hidden":false,"merged":[[43,1,43,14],[50,1,50,16],[51,1,51,16],[32,1,32,16],[33,1,33,16],[34,1,34,16],[37,1,38,16],[40,1,40,16],[41,1,41,14],[42,1,42,16]]}
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["Template design: "]
["Dionne David MSc"]
["Dr Arjen Sloots"]
[]
[]
["This template should be used strictly for study design Option 1: Testing all vaccine lots in one experiment.\n"]
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[]
[]
[]
[]
[]
[]
[]
["How to generate the PSPT results"]
[]
[null,"This worksheet will allow you to do the following tasks:"]
[null,"1) Paste the absorbance 450nm values in the sheet provided (96well format)"]
[null,"2) Create a 4 parameter logistic (4PL) curve fit from the standard data "]
[null,"3) Interpolate unknown samples from the standard curve"]
[null,"4) Calculate estimated antibody titers in Elisa Units/mL (EU/mL)"]
[null,"5) Prepare a summary of the antibody titers and geomean for each vaccine dose"]
[null,"6) Calculate the vaccine lot potency using parallel line analysis  (PLA)"]
[]
["Important information"]
[]
[" ","Before starting, ensure that the 'Solver' Add-In is installed in your version of Excel."]
[null,"Select \"Data\" in the main menu and click on 'Solver' under the Analysis group."]
[null,"If 'Solver' is not listed, install it from \"Add-Ins\" menu. Refer to Excel Help files for assistance if needed."]
[]
[]
[null,"Excel \"Solver\" analysis tool is used in conjunction with the 4PL equation to perform a non-linear least squares curve fit to the standard data."]
[]
[]
[null,"View online tutorial video for additional guidance before getting started"]
[null,"Steps: "]
[null,"(1) If you see the Security warning 'Macros have been disabled'  below the toolbar ribbon: Click on 'Enable content'."]
[null,"(2) Enter the absorbance (OD450nm) per plate in 'Step 1_Input OD450 data' sheet"]
[null,"(3) Go to worksheet tab 'Step2_Curvefit_plate XX'. Click on the 'Solver_CurveFit' button to run Excel's Solver function."]
[null,"(4) Unhide additional worksheets by right clicking on the worksheet Step 2 tab and then select: Unhide 'Step2_Curvefit_plate X'"]
[null,"(5) Repeat steps 2 to 4 for each plate."]
[null,"(6) To allow calculation of potencies in IU/vial, please fill in the potency of the used Reference vaccine in cell S1 of each PLA tab (in IU/vial)."]
[null,"(7) Fill in the name of the used Reference vaccine and particular Test vaccine in cells K3 and V3, respectively, of each PLA tab."]
[]
[null,"The 4PL equation represents the best method for fitting a calculated curve to the data"]
[null,"4PL: Y = (A-D)/(1+(X/C)^B) + D"]
[]
[null,null,null,null,null,null,null,"X = concentrations (EU/mL)"]
[null,null,null,null,null,null,null,"Y = Blanked OD450 data"]
[null,null,null,null,null,null,null,"A = Maximum asymptote"]
[null,null,null,null,null,null,null,"B = Slope"]
[null,null,null,null,null,null,null,"C = Inflection point (50% point of curve)"]
[null,null,null,null,null,null,null,"D = Minimum asymptote"]

{"sheet":"PSPT scheme Opt1","index":1,"n_rows":62,"n_cols":15,"rows_kept":62,"cut":false,"hidden":false,"merged":[[22,1,25,1],[1,3,1,14],[2,1,5,1],[6,1,9,1],[10,1,13,1],[14,1,17,1],[18,1,21,1]]}
[]
[null,"Vaccine","dose","Animal numbers"]
[null,"Reference: RWRS / WHO wP IS4 / In-house reference","10 IU/mL",1,2,3,4,5,6,7,8,9,10,11,12]
[null,null,"5 IU/mL",13,14,15,16,17,18,19,20,21,22,23,24]
[null,null,"2.5 IU/mL",25,26,27,28,29,30,31,32,33,34,35,36]
[null,null,"1.25 IU/mL",37,38,39,40,41,42,43,44,45,46,47,48]
[null,"FL1","50µl",49,50,51,52,53,54,55,56,57,58,59,60]
[null,null,"25µl",61,62,63,64,65,66,67,68,69,70,71,72]
[null,null,"12.5µl",73,74,75,76,77,78,79,80,81,82,83,84]
[null,null,"6.25µl",85,86,87,88,89,90,91,92,93,94,95,96]
[null,"FL2A","50µl",97,98,99,100,101,102,103,104,105,106,107,108]
[null,null,"25µl",109,110,111,112,113,114,115,116,117,118,119,120]
[null,null,"12.5µl",121,122,123,124,125,126,127,128,129,130,131,132]
[null,null,"6.25µl",133,134,135,136,137,138,139,140,141,142,143,144]
[null,"FL2B","50µl",145,146,147,148,149,150,151,152,153,154,155,156]
[null,null,"25µl",157,158,159,160,161,162,163,164,165,166,167,168]
[null,null,"12.5µl",169,170,171,172,173,174,175,176,177,178,179,180]
[null,null,"6.25µl",181,182,183,184,185,186,187,188,189,190,191,192]
[null,"FL3","50µl",193,194,195,196,197,198,199,200,201,202,203,204]
[null,null,"25µl",205,206,207,208,209,210,211,212,213,214,215,216]
[null,null,"12.5µl",217,218,219,220,221,222,223,224,225,226,227,228]
[null,null,"6.25µl",229,230,231,232,233,234,235,236,237,238,239,240]
[null,"FL3-alt(ered) ","50µl",241,242,243,244,245,246,247,248,249,250,251,252]
[null,null,"25µl",253,254,255,256,257,258,259,260,261,262,263,264]
[null,null,"12.5µl",265,266,267,268,269,270,271,272,273,274,275,276]
[null,null,"6.25µl",277,278,279,280,281,282,283,284,285,286,287,288]
[]
[]
[null,"ELISA plate setup (No randomisation)"]
[null,"Plate#","column 1","column 2","column 3","column 4","column 5","column 6","column 7","column 8","column 9","column 10","column 11","column 12"]
[null,1,"NC","PC","PC",1,2,3,4,5,6,7,8,9]
[null,2,"NC","PC","PC",10,11,12,13,14,15,16,17,18]
[null,3,"NC","PC","PC",19,20,21,22,23,24,25,26,27]
[null,4,"NC","PC","PC",28,29,30,31,32,33,34,35,36]
[null,5,"NC","PC","PC",37,38,39,40,41,42,43,44,45]
[null,6,"NC","PC","PC",46,47,48,49,50,51,52,53,54]
[null,7,"NC","PC","PC",55,56,57,58,59,60,61,62,63]
[null,8,"NC","PC","PC",64,65,66,67,68,69,70,71,72]
[null,9,"NC","PC","PC",73,74,75,76,77,78,79,80,81]
[null,10,"NC","PC","PC",82,83,84,85,86,87,88,89,90]
[null,11,"NC","PC","PC",91,92,93,94,95,96,97,98,99]
[null,12,"NC","PC","PC",100,101,102,103,104,105,106,107,108]
[null,13,"NC","PC","PC",109,110,111,112,113,114,115,116,117]
[null,14,"NC","PC","PC",118,119,120,121,122,123,124,125,126]
[null,15,"NC","PC","PC",127,128,129,130,131,132,133,134,135]
[null,16,"NC","PC","PC",136,137,138,139,140,141,142,143,144]
[null,17,"NC","PC","PC",145,146,147,148,149,150,151,152,153]
[null,18,"NC","PC","PC",154,155,156,157,158,159,160,161,162]
[null,19,"NC","PC","PC",163,164,165,166,167,168,169,170,171]
[null,20,"NC","PC","PC",172,173,174,175,176,177,178,179,180]
[null,21,"NC","PC","PC",181,182,183,184,185,186,187,188,189]
[null,22,"NC","PC","PC",190,191,192,193,194,195,196,197,198]
[null,23,"NC","PC","PC",199,200,201,202,203,204,205,206,207]
[null,24,"NC","PC","PC",208,209,210,211,212,213,214,215,216]
[null,25,"NC","PC","PC",217,218,219,220,221,222,223,224,225]
[null,26,"NC","PC","PC",226,227,228,229,230,231,232,233,234]
[null,27,"NC","PC","PC",235,236,237,238,239,240,241,242,243]
[null,28,"NC","PC","PC",244,245,246,247,248,249,250,251,252]
[null,29,"NC","PC","PC",253,254,255,256,257,258,259,260,261]
[null,30,"NC","PC","PC",262,263,264,265,266,267,268,269,270]
[null,31,"NC","PC","PC",271,272,273,274,275,276,277,278,279]
[null,32,"NC","PC","PC",280,281,282,283,284,285,286,287,288]

{"sheet":"Step1_Input OD450 data","index":2,"n_rows":131,"n_cols":60,"rows_kept":131,"cut":false,"hidden":false,"merged":[[3,1,6,7],[1,1,2,7]]}
[]
[null,"Step 1:"]
[]
[null,"Copy and paste absorbance 450 values for each plate in the designated wells"]
[]
[]
[]
[]
[]
[]
[]
[null,"Plate laypout",1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID"]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID"]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
[]
[]
[null,"Plate 1",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 11",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 21",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 31",1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450]
[]
[]
[null,"Plate 2",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 12",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 22",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 32",1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450]
[]
[]
[null,"Plate 3",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 13",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 23",1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450]
[]
[]
[null,"Plate 4",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 14",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 24",1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"A",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[]
[]
[null,"Plate 5",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 15",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 25",1,2,3,4,5,6,7,8,9,10,11,12]
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[]
[]
[null,"Plate 6",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 16",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 26",1,2,3,4,5,6,7,8,9,10,11,12]
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[]
[]
[null,"Plate 7",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 17",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 27",1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[]
[]
[null,"Plate 8",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 18",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 28",1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[]
[]
[null,"Plate 9",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 19",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 29",1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
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[]
[]
[null,"Plate 10",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 20",1,2,3,4,5,6,7,8,9,10,11,12,null,null,"Plate 30",1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"B",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"C",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"D",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"E",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"F",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"G",null,null,null,null,null,null,null,null,null,null,null,null,450]
[null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450,null,"H",null,null,null,null,null,null,null,null,null,null,null,null,450]

{"sheet":"Step2_Curvefit_plate 1","index":3,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C25","v":0},{"$f":"'Step1_Input OD450 data'!D25","v":0},{"$f":"'Step1_Input OD450 data'!E25","v":0},{"$f":"'Step1_Input OD450 data'!F25","v":0},{"$f":"'Step1_Input OD450 data'!G25","v":0},{"$f":"'Step1_Input OD450 data'!H25","v":0},{"$f":"'Step1_Input OD450 data'!I25","v":0},{"$f":"'Step1_Input OD450 data'!J25","v":0},{"$f":"'Step1_Input OD450 data'!K25","v":0},{"$f":"'Step1_Input OD450 data'!L25","v":0},{"$f":"'Step1_Input OD450 data'!M25","v":0},{"$f":"'Step1_Input OD450 data'!N25","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C26","v":0},{"$f":"'Step1_Input OD450 data'!D26","v":0},{"$f":"'Step1_Input OD450 data'!E26","v":0},{"$f":"'Step1_Input OD450 data'!F26","v":0},{"$f":"'Step1_Input OD450 data'!G26","v":0},{"$f":"'Step1_Input OD450 data'!H26","v":0},{"$f":"'Step1_Input OD450 data'!I26","v":0},{"$f":"'Step1_Input OD450 data'!J26","v":0},{"$f":"'Step1_Input OD450 data'!K26","v":0},{"$f":"'Step1_Input OD450 data'!L26","v":0},{"$f":"'Step1_Input OD450 data'!M26","v":0},{"$f":"'Step1_Input OD450 data'!N26","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C27","v":0},{"$f":"'Step1_Input OD450 data'!D27","v":0},{"$f":"'Step1_Input OD450 data'!E27","v":0},{"$f":"'Step1_Input OD450 data'!F27","v":0},{"$f":"'Step1_Input OD450 data'!G27","v":0},{"$f":"'Step1_Input OD450 data'!H27","v":0},{"$f":"'Step1_Input OD450 data'!I27","v":0},{"$f":"'Step1_Input OD450 data'!J27","v":0},{"$f":"'Step1_Input OD450 data'!K27","v":0},{"$f":"'Step1_Input OD450 data'!L27","v":0},{"$f":"'Step1_Input OD450 data'!M27","v":0},{"$f":"'Step1_Input OD450 data'!N27","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C28","v":0},{"$f":"'Step1_Input OD450 data'!D28","v":0},{"$f":"'Step1_Input OD450 data'!E28","v":0},{"$f":"'Step1_Input OD450 data'!F28","v":0},{"$f":"'Step1_Input OD450 data'!G28","v":0},{"$f":"'Step1_Input OD450 data'!H28","v":0},{"$f":"'Step1_Input OD450 data'!I28","v":0},{"$f":"'Step1_Input OD450 data'!J28","v":0},{"$f":"'Step1_Input OD450 data'!K28","v":0},{"$f":"'Step1_Input OD450 data'!L28","v":0},{"$f":"'Step1_Input OD450 data'!M28","v":0},{"$f":"'Step1_Input OD450 data'!N28","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C29","v":0},{"$f":"'Step1_Input OD450 data'!D29","v":0},{"$f":"'Step1_Input OD450 data'!E29","v":0},{"$f":"'Step1_Input OD450 data'!F29","v":0},{"$f":"'Step1_Input OD450 data'!G29","v":0},{"$f":"'Step1_Input OD450 data'!H29","v":0},{"$f":"'Step1_Input OD450 data'!I29","v":0},{"$f":"'Step1_Input OD450 data'!J29","v":0},{"$f":"'Step1_Input OD450 data'!K29","v":0},{"$f":"'Step1_Input OD450 data'!L29","v":0},{"$f":"'Step1_Input OD450 data'!M29","v":0},{"$f":"'Step1_Input OD450 data'!N29","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C30","v":0},{"$f":"'Step1_Input OD450 data'!D30","v":0},{"$f":"'Step1_Input OD450 data'!E30","v":0},{"$f":"'Step1_Input OD450 data'!F30","v":0},{"$f":"'Step1_Input OD450 data'!G30","v":0},{"$f":"'Step1_Input OD450 data'!H30","v":0},{"$f":"'Step1_Input OD450 data'!I30","v":0},{"$f":"'Step1_Input OD450 data'!J30","v":0},{"$f":"'Step1_Input OD450 data'!K30","v":0},{"$f":"'Step1_Input OD450 data'!L30","v":0},{"$f":"'Step1_Input OD450 data'!M30","v":0},{"$f":"'Step1_Input OD450 data'!N30","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C31","v":0},{"$f":"'Step1_Input OD450 data'!D31","v":0},{"$f":"'Step1_Input OD450 data'!E31","v":0},{"$f":"'Step1_Input OD450 data'!F31","v":0},{"$f":"'Step1_Input OD450 data'!G31","v":0},{"$f":"'Step1_Input OD450 data'!H31","v":0},{"$f":"'Step1_Input OD450 data'!I31","v":0},{"$f":"'Step1_Input OD450 data'!J31","v":0},{"$f":"'Step1_Input OD450 data'!K31","v":0},{"$f":"'Step1_Input OD450 data'!L31","v":0},{"$f":"'Step1_Input OD450 data'!M31","v":0},{"$f":"'Step1_Input OD450 data'!N31","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C32","v":0},{"$f":"'Step1_Input OD450 data'!D32","v":0},{"$f":"'Step1_Input OD450 data'!E32","v":0},{"$f":"'Step1_Input OD450 data'!F32","v":0},{"$f":"'Step1_Input OD450 data'!G32","v":0},{"$f":"'Step1_Input OD450 data'!H32","v":0},{"$f":"'Step1_Input OD450 data'!I32","v":0},{"$f":"'Step1_Input OD450 data'!J32","v":0},{"$f":"'Step1_Input OD450 data'!K32","v":0},{"$f":"'Step1_Input OD450 data'!L32","v":0},{"$f":"'Step1_Input OD450 data'!M32","v":0},{"$f":"'Step1_Input OD450 data'!N32","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",{"$f":"IF(ISNUMBER(C36),C36-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",{"$f":"IF(ISNUMBER(C37),C37-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",{"$f":"IF(ISNUMBER(C38),C38-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",{"$f":"IF(ISNUMBER(C39),C39-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",{"$f":"IF(ISNUMBER(C40),C40-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",{"$f":"IF(ISNUMBER(C41),C41-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,1,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,2,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,3,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,4,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,5,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,6,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,7,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,8,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,9,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 2","index":4,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C36","v":0},{"$f":"'Step1_Input OD450 data'!D36","v":0},{"$f":"'Step1_Input OD450 data'!E36","v":0},{"$f":"'Step1_Input OD450 data'!F36","v":0},{"$f":"'Step1_Input OD450 data'!G36","v":0},{"$f":"'Step1_Input OD450 data'!H36","v":0},{"$f":"'Step1_Input OD450 data'!I36","v":0},{"$f":"'Step1_Input OD450 data'!J36","v":0},{"$f":"'Step1_Input OD450 data'!K36","v":0},{"$f":"'Step1_Input OD450 data'!L36","v":0},{"$f":"'Step1_Input OD450 data'!M36","v":0},{"$f":"'Step1_Input OD450 data'!N36","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C37","v":0},{"$f":"'Step1_Input OD450 data'!D37","v":0},{"$f":"'Step1_Input OD450 data'!E37","v":0},{"$f":"'Step1_Input OD450 data'!F37","v":0},{"$f":"'Step1_Input OD450 data'!G37","v":0},{"$f":"'Step1_Input OD450 data'!H37","v":0},{"$f":"'Step1_Input OD450 data'!I37","v":0},{"$f":"'Step1_Input OD450 data'!J37","v":0},{"$f":"'Step1_Input OD450 data'!K37","v":0},{"$f":"'Step1_Input OD450 data'!L37","v":0},{"$f":"'Step1_Input OD450 data'!M37","v":0},{"$f":"'Step1_Input OD450 data'!N37","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C38","v":0},{"$f":"'Step1_Input OD450 data'!D38","v":0},{"$f":"'Step1_Input OD450 data'!E38","v":0},{"$f":"'Step1_Input OD450 data'!F38","v":0},{"$f":"'Step1_Input OD450 data'!G38","v":0},{"$f":"'Step1_Input OD450 data'!H38","v":0},{"$f":"'Step1_Input OD450 data'!I38","v":0},{"$f":"'Step1_Input OD450 data'!J38","v":0},{"$f":"'Step1_Input OD450 data'!K38","v":0},{"$f":"'Step1_Input OD450 data'!L38","v":0},{"$f":"'Step1_Input OD450 data'!M38","v":0},{"$f":"'Step1_Input OD450 data'!N38","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C39","v":0},{"$f":"'Step1_Input OD450 data'!D39","v":0},{"$f":"'Step1_Input OD450 data'!E39","v":0},{"$f":"'Step1_Input OD450 data'!F39","v":0},{"$f":"'Step1_Input OD450 data'!G39","v":0},{"$f":"'Step1_Input OD450 data'!H39","v":0},{"$f":"'Step1_Input OD450 data'!I39","v":0},{"$f":"'Step1_Input OD450 data'!J39","v":0},{"$f":"'Step1_Input OD450 data'!K39","v":0},{"$f":"'Step1_Input OD450 data'!L39","v":0},{"$f":"'Step1_Input OD450 data'!M39","v":0},{"$f":"'Step1_Input OD450 data'!N39","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C40","v":0},{"$f":"'Step1_Input OD450 data'!D40","v":0},{"$f":"'Step1_Input OD450 data'!E40","v":0},{"$f":"'Step1_Input OD450 data'!F40","v":0},{"$f":"'Step1_Input OD450 data'!G40","v":0},{"$f":"'Step1_Input OD450 data'!H40","v":0},{"$f":"'Step1_Input OD450 data'!I40","v":0},{"$f":"'Step1_Input OD450 data'!J40","v":0},{"$f":"'Step1_Input OD450 data'!K40","v":0},{"$f":"'Step1_Input OD450 data'!L40","v":0},{"$f":"'Step1_Input OD450 data'!M40","v":0},{"$f":"'Step1_Input OD450 data'!N40","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C41","v":0},{"$f":"'Step1_Input OD450 data'!D41","v":0},{"$f":"'Step1_Input OD450 data'!E41","v":0},{"$f":"'Step1_Input OD450 data'!F41","v":0},{"$f":"'Step1_Input OD450 data'!G41","v":0},{"$f":"'Step1_Input OD450 data'!H41","v":0},{"$f":"'Step1_Input OD450 data'!I41","v":0},{"$f":"'Step1_Input OD450 data'!J41","v":0},{"$f":"'Step1_Input OD450 data'!K41","v":0},{"$f":"'Step1_Input OD450 data'!L41","v":0},{"$f":"'Step1_Input OD450 data'!M41","v":0},{"$f":"'Step1_Input OD450 data'!N41","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C42","v":0},{"$f":"'Step1_Input OD450 data'!D42","v":0},{"$f":"'Step1_Input OD450 data'!E42","v":0},{"$f":"'Step1_Input OD450 data'!F42","v":0},{"$f":"'Step1_Input OD450 data'!G42","v":0},{"$f":"'Step1_Input OD450 data'!H42","v":0},{"$f":"'Step1_Input OD450 data'!I42","v":0},{"$f":"'Step1_Input OD450 data'!J42","v":0},{"$f":"'Step1_Input OD450 data'!K42","v":0},{"$f":"'Step1_Input OD450 data'!L42","v":0},{"$f":"'Step1_Input OD450 data'!M42","v":0},{"$f":"'Step1_Input OD450 data'!N42","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C43","v":0},{"$f":"'Step1_Input OD450 data'!D43","v":0},{"$f":"'Step1_Input OD450 data'!E43","v":0},{"$f":"'Step1_Input OD450 data'!F43","v":0},{"$f":"'Step1_Input OD450 data'!G43","v":0},{"$f":"'Step1_Input OD450 data'!H43","v":0},{"$f":"'Step1_Input OD450 data'!I43","v":0},{"$f":"'Step1_Input OD450 data'!J43","v":0},{"$f":"'Step1_Input OD450 data'!K43","v":0},{"$f":"'Step1_Input OD450 data'!L43","v":0},{"$f":"'Step1_Input OD450 data'!M43","v":0},{"$f":"'Step1_Input OD450 data'!N43","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,{"$f":"J166","v":""},"","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","",{"$f":"J199","v":""},"","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,10,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,11,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,12,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,13,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,14,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,15,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,16,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,17,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,18,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 3","index":5,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C47","v":0},{"$f":"'Step1_Input OD450 data'!D47","v":0},{"$f":"'Step1_Input OD450 data'!E47","v":0},{"$f":"'Step1_Input OD450 data'!F47","v":0},{"$f":"'Step1_Input OD450 data'!G47","v":0},{"$f":"'Step1_Input OD450 data'!H47","v":0},{"$f":"'Step1_Input OD450 data'!I47","v":0},{"$f":"'Step1_Input OD450 data'!J47","v":0},{"$f":"'Step1_Input OD450 data'!K47","v":0},{"$f":"'Step1_Input OD450 data'!L47","v":0},{"$f":"'Step1_Input OD450 data'!M47","v":0},{"$f":"'Step1_Input OD450 data'!N47","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C48","v":0},{"$f":"'Step1_Input OD450 data'!D48","v":0},{"$f":"'Step1_Input OD450 data'!E48","v":0},{"$f":"'Step1_Input OD450 data'!F48","v":0},{"$f":"'Step1_Input OD450 data'!G48","v":0},{"$f":"'Step1_Input OD450 data'!H48","v":0},{"$f":"'Step1_Input OD450 data'!I48","v":0},{"$f":"'Step1_Input OD450 data'!J48","v":0},{"$f":"'Step1_Input OD450 data'!K48","v":0},{"$f":"'Step1_Input OD450 data'!L48","v":0},{"$f":"'Step1_Input OD450 data'!M48","v":0},{"$f":"'Step1_Input OD450 data'!N48","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C49","v":0},{"$f":"'Step1_Input OD450 data'!D49","v":0},{"$f":"'Step1_Input OD450 data'!E49","v":0},{"$f":"'Step1_Input OD450 data'!F49","v":0},{"$f":"'Step1_Input OD450 data'!G49","v":0},{"$f":"'Step1_Input OD450 data'!H49","v":0},{"$f":"'Step1_Input OD450 data'!I49","v":0},{"$f":"'Step1_Input OD450 data'!J49","v":0},{"$f":"'Step1_Input OD450 data'!K49","v":0},{"$f":"'Step1_Input OD450 data'!L49","v":0},{"$f":"'Step1_Input OD450 data'!M49","v":0},{"$f":"'Step1_Input OD450 data'!N49","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C50","v":0},{"$f":"'Step1_Input OD450 data'!D50","v":0},{"$f":"'Step1_Input OD450 data'!E50","v":0},{"$f":"'Step1_Input OD450 data'!F50","v":0},{"$f":"'Step1_Input OD450 data'!G50","v":0},{"$f":"'Step1_Input OD450 data'!H50","v":0},{"$f":"'Step1_Input OD450 data'!I50","v":0},{"$f":"'Step1_Input OD450 data'!J50","v":0},{"$f":"'Step1_Input OD450 data'!K50","v":0},{"$f":"'Step1_Input OD450 data'!L50","v":0},{"$f":"'Step1_Input OD450 data'!M50","v":0},{"$f":"'Step1_Input OD450 data'!N50","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C51","v":0},{"$f":"'Step1_Input OD450 data'!D51","v":0},{"$f":"'Step1_Input OD450 data'!E51","v":0},{"$f":"'Step1_Input OD450 data'!F51","v":0},{"$f":"'Step1_Input OD450 data'!G51","v":0},{"$f":"'Step1_Input OD450 data'!H51","v":0},{"$f":"'Step1_Input OD450 data'!I51","v":0},{"$f":"'Step1_Input OD450 data'!J51","v":0},{"$f":"'Step1_Input OD450 data'!K51","v":0},{"$f":"'Step1_Input OD450 data'!L51","v":0},{"$f":"'Step1_Input OD450 data'!M51","v":0},{"$f":"'Step1_Input OD450 data'!N51","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C52","v":0},{"$f":"'Step1_Input OD450 data'!D52","v":0},{"$f":"'Step1_Input OD450 data'!E52","v":0},{"$f":"'Step1_Input OD450 data'!F52","v":0},{"$f":"'Step1_Input OD450 data'!G52","v":0},{"$f":"'Step1_Input OD450 data'!H52","v":0},{"$f":"'Step1_Input OD450 data'!I52","v":0},{"$f":"'Step1_Input OD450 data'!J52","v":0},{"$f":"'Step1_Input OD450 data'!K52","v":0},{"$f":"'Step1_Input OD450 data'!L52","v":0},{"$f":"'Step1_Input OD450 data'!M52","v":0},{"$f":"'Step1_Input OD450 data'!N52","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C53","v":0},{"$f":"'Step1_Input OD450 data'!D53","v":0},{"$f":"'Step1_Input OD450 data'!E53","v":0},{"$f":"'Step1_Input OD450 data'!F53","v":0},{"$f":"'Step1_Input OD450 data'!G53","v":0},{"$f":"'Step1_Input OD450 data'!H53","v":0},{"$f":"'Step1_Input OD450 data'!I53","v":0},{"$f":"'Step1_Input OD450 data'!J53","v":0},{"$f":"'Step1_Input OD450 data'!K53","v":0},{"$f":"'Step1_Input OD450 data'!L53","v":0},{"$f":"'Step1_Input OD450 data'!M53","v":0},{"$f":"'Step1_Input OD450 data'!N53","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C54","v":0},{"$f":"'Step1_Input OD450 data'!D54","v":0},{"$f":"'Step1_Input OD450 data'!E54","v":0},{"$f":"'Step1_Input OD450 data'!F54","v":0},{"$f":"'Step1_Input OD450 data'!G54","v":0},{"$f":"'Step1_Input OD450 data'!H54","v":0},{"$f":"'Step1_Input OD450 data'!I54","v":0},{"$f":"'Step1_Input OD450 data'!J54","v":0},{"$f":"'Step1_Input OD450 data'!K54","v":0},{"$f":"'Step1_Input OD450 data'!L54","v":0},{"$f":"'Step1_Input OD450 data'!M54","v":0},{"$f":"'Step1_Input OD450 data'!N54","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,19,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,20,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,21,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,22,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,23,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,24,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,25,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,26,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,27,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 4","index":6,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C58","v":0},{"$f":"'Step1_Input OD450 data'!D58","v":0},{"$f":"'Step1_Input OD450 data'!E58","v":0},{"$f":"'Step1_Input OD450 data'!F58","v":0},{"$f":"'Step1_Input OD450 data'!G58","v":0},{"$f":"'Step1_Input OD450 data'!H58","v":0},{"$f":"'Step1_Input OD450 data'!I58","v":0},{"$f":"'Step1_Input OD450 data'!J58","v":0},{"$f":"'Step1_Input OD450 data'!K58","v":0},{"$f":"'Step1_Input OD450 data'!L58","v":0},{"$f":"'Step1_Input OD450 data'!M58","v":0},{"$f":"'Step1_Input OD450 data'!N58","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C59","v":0},{"$f":"'Step1_Input OD450 data'!D59","v":0},{"$f":"'Step1_Input OD450 data'!E59","v":0},{"$f":"'Step1_Input OD450 data'!F59","v":0},{"$f":"'Step1_Input OD450 data'!G59","v":0},{"$f":"'Step1_Input OD450 data'!H59","v":0},{"$f":"'Step1_Input OD450 data'!I59","v":0},{"$f":"'Step1_Input OD450 data'!J59","v":0},{"$f":"'Step1_Input OD450 data'!K59","v":0},{"$f":"'Step1_Input OD450 data'!L59","v":0},{"$f":"'Step1_Input OD450 data'!M59","v":0},{"$f":"'Step1_Input OD450 data'!N59","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C60","v":0},{"$f":"'Step1_Input OD450 data'!D60","v":0},{"$f":"'Step1_Input OD450 data'!E60","v":0},{"$f":"'Step1_Input OD450 data'!F60","v":0},{"$f":"'Step1_Input OD450 data'!G60","v":0},{"$f":"'Step1_Input OD450 data'!H60","v":0},{"$f":"'Step1_Input OD450 data'!I60","v":0},{"$f":"'Step1_Input OD450 data'!J60","v":0},{"$f":"'Step1_Input OD450 data'!K60","v":0},{"$f":"'Step1_Input OD450 data'!L60","v":0},{"$f":"'Step1_Input OD450 data'!M60","v":0},{"$f":"'Step1_Input OD450 data'!N60","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C61","v":0},{"$f":"'Step1_Input OD450 data'!D61","v":0},{"$f":"'Step1_Input OD450 data'!E61","v":0},{"$f":"'Step1_Input OD450 data'!F61","v":0},{"$f":"'Step1_Input OD450 data'!G61","v":0},{"$f":"'Step1_Input OD450 data'!H61","v":0},{"$f":"'Step1_Input OD450 data'!I61","v":0},{"$f":"'Step1_Input OD450 data'!J61","v":0},{"$f":"'Step1_Input OD450 data'!K61","v":0},{"$f":"'Step1_Input OD450 data'!L61","v":0},{"$f":"'Step1_Input OD450 data'!M61","v":0},{"$f":"'Step1_Input OD450 data'!N61","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C62","v":0},{"$f":"'Step1_Input OD450 data'!D62","v":0},{"$f":"'Step1_Input OD450 data'!E62","v":0},{"$f":"'Step1_Input OD450 data'!F62","v":0},{"$f":"'Step1_Input OD450 data'!G62","v":0},{"$f":"'Step1_Input OD450 data'!H62","v":0},{"$f":"'Step1_Input OD450 data'!I62","v":0},{"$f":"'Step1_Input OD450 data'!J62","v":0},{"$f":"'Step1_Input OD450 data'!K62","v":0},{"$f":"'Step1_Input OD450 data'!L62","v":0},{"$f":"'Step1_Input OD450 data'!M62","v":0},{"$f":"'Step1_Input OD450 data'!N62","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C63","v":0},{"$f":"'Step1_Input OD450 data'!D63","v":0},{"$f":"'Step1_Input OD450 data'!E63","v":0},{"$f":"'Step1_Input OD450 data'!F63","v":0},{"$f":"'Step1_Input OD450 data'!G63","v":0},{"$f":"'Step1_Input OD450 data'!H63","v":0},{"$f":"'Step1_Input OD450 data'!I63","v":0},{"$f":"'Step1_Input OD450 data'!J63","v":0},{"$f":"'Step1_Input OD450 data'!K63","v":0},{"$f":"'Step1_Input OD450 data'!L63","v":0},{"$f":"'Step1_Input OD450 data'!M63","v":0},{"$f":"'Step1_Input OD450 data'!N63","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C64","v":0},{"$f":"'Step1_Input OD450 data'!D64","v":0},{"$f":"'Step1_Input OD450 data'!E64","v":0},{"$f":"'Step1_Input OD450 data'!F64","v":0},{"$f":"'Step1_Input OD450 data'!G64","v":0},{"$f":"'Step1_Input OD450 data'!H64","v":0},{"$f":"'Step1_Input OD450 data'!I64","v":0},{"$f":"'Step1_Input OD450 data'!J64","v":0},{"$f":"'Step1_Input OD450 data'!K64","v":0},{"$f":"'Step1_Input OD450 data'!L64","v":0},{"$f":"'Step1_Input OD450 data'!M64","v":0},{"$f":"'Step1_Input OD450 data'!N64","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C65","v":0},{"$f":"'Step1_Input OD450 data'!D65","v":0},{"$f":"'Step1_Input OD450 data'!E65","v":0},{"$f":"'Step1_Input OD450 data'!F65","v":0},{"$f":"'Step1_Input OD450 data'!G65","v":0},{"$f":"'Step1_Input OD450 data'!H65","v":0},{"$f":"'Step1_Input OD450 data'!I65","v":0},{"$f":"'Step1_Input OD450 data'!J65","v":0},{"$f":"'Step1_Input OD450 data'!K65","v":0},{"$f":"'Step1_Input OD450 data'!L65","v":0},{"$f":"'Step1_Input OD450 data'!M65","v":0},{"$f":"'Step1_Input OD450 data'!N65","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,28,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,29,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,30,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,31,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,32,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,33,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,34,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,35,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,36,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 5","index":7,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C69","v":0},{"$f":"'Step1_Input OD450 data'!D69","v":0},{"$f":"'Step1_Input OD450 data'!E69","v":0},{"$f":"'Step1_Input OD450 data'!F69","v":0},{"$f":"'Step1_Input OD450 data'!G69","v":0},{"$f":"'Step1_Input OD450 data'!H69","v":0},{"$f":"'Step1_Input OD450 data'!I69","v":0},{"$f":"'Step1_Input OD450 data'!J69","v":0},{"$f":"'Step1_Input OD450 data'!K69","v":0},{"$f":"'Step1_Input OD450 data'!L69","v":0},{"$f":"'Step1_Input OD450 data'!M69","v":0},{"$f":"'Step1_Input OD450 data'!N69","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C70","v":0},{"$f":"'Step1_Input OD450 data'!D70","v":0},{"$f":"'Step1_Input OD450 data'!E70","v":0},{"$f":"'Step1_Input OD450 data'!F70","v":0},{"$f":"'Step1_Input OD450 data'!G70","v":0},{"$f":"'Step1_Input OD450 data'!H70","v":0},{"$f":"'Step1_Input OD450 data'!I70","v":0},{"$f":"'Step1_Input OD450 data'!J70","v":0},{"$f":"'Step1_Input OD450 data'!K70","v":0},{"$f":"'Step1_Input OD450 data'!L70","v":0},{"$f":"'Step1_Input OD450 data'!M70","v":0},{"$f":"'Step1_Input OD450 data'!N70","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C71","v":0},{"$f":"'Step1_Input OD450 data'!D71","v":0},{"$f":"'Step1_Input OD450 data'!E71","v":0},{"$f":"'Step1_Input OD450 data'!F71","v":0},{"$f":"'Step1_Input OD450 data'!G71","v":0},{"$f":"'Step1_Input OD450 data'!H71","v":0},{"$f":"'Step1_Input OD450 data'!I71","v":0},{"$f":"'Step1_Input OD450 data'!J71","v":0},{"$f":"'Step1_Input OD450 data'!K71","v":0},{"$f":"'Step1_Input OD450 data'!L71","v":0},{"$f":"'Step1_Input OD450 data'!M71","v":0},{"$f":"'Step1_Input OD450 data'!N71","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C72","v":0},{"$f":"'Step1_Input OD450 data'!D72","v":0},{"$f":"'Step1_Input OD450 data'!E72","v":0},{"$f":"'Step1_Input OD450 data'!F72","v":0},{"$f":"'Step1_Input OD450 data'!G72","v":0},{"$f":"'Step1_Input OD450 data'!H72","v":0},{"$f":"'Step1_Input OD450 data'!I72","v":0},{"$f":"'Step1_Input OD450 data'!J72","v":0},{"$f":"'Step1_Input OD450 data'!K72","v":0},{"$f":"'Step1_Input OD450 data'!L72","v":0},{"$f":"'Step1_Input OD450 data'!M72","v":0},{"$f":"'Step1_Input OD450 data'!N72","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C73","v":0},{"$f":"'Step1_Input OD450 data'!D73","v":0},{"$f":"'Step1_Input OD450 data'!E73","v":0},{"$f":"'Step1_Input OD450 data'!F73","v":0},{"$f":"'Step1_Input OD450 data'!G73","v":0},{"$f":"'Step1_Input OD450 data'!H73","v":0},{"$f":"'Step1_Input OD450 data'!I73","v":0},{"$f":"'Step1_Input OD450 data'!J73","v":0},{"$f":"'Step1_Input OD450 data'!K73","v":0},{"$f":"'Step1_Input OD450 data'!L73","v":0},{"$f":"'Step1_Input OD450 data'!M73","v":0},{"$f":"'Step1_Input OD450 data'!N73","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C74","v":0},{"$f":"'Step1_Input OD450 data'!D74","v":0},{"$f":"'Step1_Input OD450 data'!E74","v":0},{"$f":"'Step1_Input OD450 data'!F74","v":0},{"$f":"'Step1_Input OD450 data'!G74","v":0},{"$f":"'Step1_Input OD450 data'!H74","v":0},{"$f":"'Step1_Input OD450 data'!I74","v":0},{"$f":"'Step1_Input OD450 data'!J74","v":0},{"$f":"'Step1_Input OD450 data'!K74","v":0},{"$f":"'Step1_Input OD450 data'!L74","v":0},{"$f":"'Step1_Input OD450 data'!M74","v":0},{"$f":"'Step1_Input OD450 data'!N74","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C75","v":0},{"$f":"'Step1_Input OD450 data'!D75","v":0},{"$f":"'Step1_Input OD450 data'!E75","v":0},{"$f":"'Step1_Input OD450 data'!F75","v":0},{"$f":"'Step1_Input OD450 data'!G75","v":0},{"$f":"'Step1_Input OD450 data'!H75","v":0},{"$f":"'Step1_Input OD450 data'!I75","v":0},{"$f":"'Step1_Input OD450 data'!J75","v":0},{"$f":"'Step1_Input OD450 data'!K75","v":0},{"$f":"'Step1_Input OD450 data'!L75","v":0},{"$f":"'Step1_Input OD450 data'!M75","v":0},{"$f":"'Step1_Input OD450 data'!N75","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C76","v":0},{"$f":"'Step1_Input OD450 data'!D76","v":0},{"$f":"'Step1_Input OD450 data'!E76","v":0},{"$f":"'Step1_Input OD450 data'!F76","v":0},{"$f":"'Step1_Input OD450 data'!G76","v":0},{"$f":"'Step1_Input OD450 data'!H76","v":0},{"$f":"'Step1_Input OD450 data'!I76","v":0},{"$f":"'Step1_Input OD450 data'!J76","v":0},{"$f":"'Step1_Input OD450 data'!K76","v":0},{"$f":"'Step1_Input OD450 data'!L76","v":0},{"$f":"'Step1_Input OD450 data'!M76","v":0},{"$f":"'Step1_Input OD450 data'!N76","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,37,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,38,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,39,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,40,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,41,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,42,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,43,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,44,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,45,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 6","index":8,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C80","v":0},{"$f":"'Step1_Input OD450 data'!D80","v":0},{"$f":"'Step1_Input OD450 data'!E80","v":0},{"$f":"'Step1_Input OD450 data'!F80","v":0},{"$f":"'Step1_Input OD450 data'!G80","v":0},{"$f":"'Step1_Input OD450 data'!H80","v":0},{"$f":"'Step1_Input OD450 data'!I80","v":0},{"$f":"'Step1_Input OD450 data'!J80","v":0},{"$f":"'Step1_Input OD450 data'!K80","v":0},{"$f":"'Step1_Input OD450 data'!L80","v":0},{"$f":"'Step1_Input OD450 data'!M80","v":0},{"$f":"'Step1_Input OD450 data'!N80","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C81","v":0},{"$f":"'Step1_Input OD450 data'!D81","v":0},{"$f":"'Step1_Input OD450 data'!E81","v":0},{"$f":"'Step1_Input OD450 data'!F81","v":0},{"$f":"'Step1_Input OD450 data'!G81","v":0},{"$f":"'Step1_Input OD450 data'!H81","v":0},{"$f":"'Step1_Input OD450 data'!I81","v":0},{"$f":"'Step1_Input OD450 data'!J81","v":0},{"$f":"'Step1_Input OD450 data'!K81","v":0},{"$f":"'Step1_Input OD450 data'!L81","v":0},{"$f":"'Step1_Input OD450 data'!M81","v":0},{"$f":"'Step1_Input OD450 data'!N81","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C82","v":0},{"$f":"'Step1_Input OD450 data'!D82","v":0},{"$f":"'Step1_Input OD450 data'!E82","v":0},{"$f":"'Step1_Input OD450 data'!F82","v":0},{"$f":"'Step1_Input OD450 data'!G82","v":0},{"$f":"'Step1_Input OD450 data'!H82","v":0},{"$f":"'Step1_Input OD450 data'!I82","v":0},{"$f":"'Step1_Input OD450 data'!J82","v":0},{"$f":"'Step1_Input OD450 data'!K82","v":0},{"$f":"'Step1_Input OD450 data'!L82","v":0},{"$f":"'Step1_Input OD450 data'!M82","v":0},{"$f":"'Step1_Input OD450 data'!N82","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C83","v":0},{"$f":"'Step1_Input OD450 data'!D83","v":0},{"$f":"'Step1_Input OD450 data'!E83","v":0},{"$f":"'Step1_Input OD450 data'!F83","v":0},{"$f":"'Step1_Input OD450 data'!G83","v":0},{"$f":"'Step1_Input OD450 data'!H83","v":0},{"$f":"'Step1_Input OD450 data'!I83","v":0},{"$f":"'Step1_Input OD450 data'!J83","v":0},{"$f":"'Step1_Input OD450 data'!K83","v":0},{"$f":"'Step1_Input OD450 data'!L83","v":0},{"$f":"'Step1_Input OD450 data'!M83","v":0},{"$f":"'Step1_Input OD450 data'!N83","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C84","v":0},{"$f":"'Step1_Input OD450 data'!D84","v":0},{"$f":"'Step1_Input OD450 data'!E84","v":0},{"$f":"'Step1_Input OD450 data'!F84","v":0},{"$f":"'Step1_Input OD450 data'!G84","v":0},{"$f":"'Step1_Input OD450 data'!H84","v":0},{"$f":"'Step1_Input OD450 data'!I84","v":0},{"$f":"'Step1_Input OD450 data'!J84","v":0},{"$f":"'Step1_Input OD450 data'!K84","v":0},{"$f":"'Step1_Input OD450 data'!L84","v":0},{"$f":"'Step1_Input OD450 data'!M84","v":0},{"$f":"'Step1_Input OD450 data'!N84","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C85","v":0},{"$f":"'Step1_Input OD450 data'!D85","v":0},{"$f":"'Step1_Input OD450 data'!E85","v":0},{"$f":"'Step1_Input OD450 data'!F85","v":0},{"$f":"'Step1_Input OD450 data'!G85","v":0},{"$f":"'Step1_Input OD450 data'!H85","v":0},{"$f":"'Step1_Input OD450 data'!I85","v":0},{"$f":"'Step1_Input OD450 data'!J85","v":0},{"$f":"'Step1_Input OD450 data'!K85","v":0},{"$f":"'Step1_Input OD450 data'!L85","v":0},{"$f":"'Step1_Input OD450 data'!M85","v":0},{"$f":"'Step1_Input OD450 data'!N85","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C86","v":0},{"$f":"'Step1_Input OD450 data'!D86","v":0},{"$f":"'Step1_Input OD450 data'!E86","v":0},{"$f":"'Step1_Input OD450 data'!F86","v":0},{"$f":"'Step1_Input OD450 data'!G86","v":0},{"$f":"'Step1_Input OD450 data'!H86","v":0},{"$f":"'Step1_Input OD450 data'!I86","v":0},{"$f":"'Step1_Input OD450 data'!J86","v":0},{"$f":"'Step1_Input OD450 data'!K86","v":0},{"$f":"'Step1_Input OD450 data'!L86","v":0},{"$f":"'Step1_Input OD450 data'!M86","v":0},{"$f":"'Step1_Input OD450 data'!N86","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C87","v":0},{"$f":"'Step1_Input OD450 data'!D87","v":0},{"$f":"'Step1_Input OD450 data'!E87","v":0},{"$f":"'Step1_Input OD450 data'!F87","v":0},{"$f":"'Step1_Input OD450 data'!G87","v":0},{"$f":"'Step1_Input OD450 data'!H87","v":0},{"$f":"'Step1_Input OD450 data'!I87","v":0},{"$f":"'Step1_Input OD450 data'!J87","v":0},{"$f":"'Step1_Input OD450 data'!K87","v":0},{"$f":"'Step1_Input OD450 data'!L87","v":0},{"$f":"'Step1_Input OD450 data'!M87","v":0},{"$f":"'Step1_Input OD450 data'!N87","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,46,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,47,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,48,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,49,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,50,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,51,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,52,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,53,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,54,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 7","index":9,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C91","v":0},{"$f":"'Step1_Input OD450 data'!D91","v":0},{"$f":"'Step1_Input OD450 data'!E91","v":0},{"$f":"'Step1_Input OD450 data'!F91","v":0},{"$f":"'Step1_Input OD450 data'!G91","v":0},{"$f":"'Step1_Input OD450 data'!H91","v":0},{"$f":"'Step1_Input OD450 data'!I91","v":0},{"$f":"'Step1_Input OD450 data'!J91","v":0},{"$f":"'Step1_Input OD450 data'!K91","v":0},{"$f":"'Step1_Input OD450 data'!L91","v":0},{"$f":"'Step1_Input OD450 data'!M91","v":0},{"$f":"'Step1_Input OD450 data'!N91","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C92","v":0},{"$f":"'Step1_Input OD450 data'!D92","v":0},{"$f":"'Step1_Input OD450 data'!E92","v":0},{"$f":"'Step1_Input OD450 data'!F92","v":0},{"$f":"'Step1_Input OD450 data'!G92","v":0},{"$f":"'Step1_Input OD450 data'!H92","v":0},{"$f":"'Step1_Input OD450 data'!I92","v":0},{"$f":"'Step1_Input OD450 data'!J92","v":0},{"$f":"'Step1_Input OD450 data'!K92","v":0},{"$f":"'Step1_Input OD450 data'!L92","v":0},{"$f":"'Step1_Input OD450 data'!M92","v":0},{"$f":"'Step1_Input OD450 data'!N92","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C93","v":0},{"$f":"'Step1_Input OD450 data'!D93","v":0},{"$f":"'Step1_Input OD450 data'!E93","v":0},{"$f":"'Step1_Input OD450 data'!F93","v":0},{"$f":"'Step1_Input OD450 data'!G93","v":0},{"$f":"'Step1_Input OD450 data'!H93","v":0},{"$f":"'Step1_Input OD450 data'!I93","v":0},{"$f":"'Step1_Input OD450 data'!J93","v":0},{"$f":"'Step1_Input OD450 data'!K93","v":0},{"$f":"'Step1_Input OD450 data'!L93","v":0},{"$f":"'Step1_Input OD450 data'!M93","v":0},{"$f":"'Step1_Input OD450 data'!N93","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C94","v":0},{"$f":"'Step1_Input OD450 data'!D94","v":0},{"$f":"'Step1_Input OD450 data'!E94","v":0},{"$f":"'Step1_Input OD450 data'!F94","v":0},{"$f":"'Step1_Input OD450 data'!G94","v":0},{"$f":"'Step1_Input OD450 data'!H94","v":0},{"$f":"'Step1_Input OD450 data'!I94","v":0},{"$f":"'Step1_Input OD450 data'!J94","v":0},{"$f":"'Step1_Input OD450 data'!K94","v":0},{"$f":"'Step1_Input OD450 data'!L94","v":0},{"$f":"'Step1_Input OD450 data'!M94","v":0},{"$f":"'Step1_Input OD450 data'!N94","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C95","v":0},{"$f":"'Step1_Input OD450 data'!D95","v":0},{"$f":"'Step1_Input OD450 data'!E95","v":0},{"$f":"'Step1_Input OD450 data'!F95","v":0},{"$f":"'Step1_Input OD450 data'!G95","v":0},{"$f":"'Step1_Input OD450 data'!H95","v":0},{"$f":"'Step1_Input OD450 data'!I95","v":0},{"$f":"'Step1_Input OD450 data'!J95","v":0},{"$f":"'Step1_Input OD450 data'!K95","v":0},{"$f":"'Step1_Input OD450 data'!L95","v":0},{"$f":"'Step1_Input OD450 data'!M95","v":0},{"$f":"'Step1_Input OD450 data'!N95","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C96","v":0},{"$f":"'Step1_Input OD450 data'!D96","v":0},{"$f":"'Step1_Input OD450 data'!E96","v":0},{"$f":"'Step1_Input OD450 data'!F96","v":0},{"$f":"'Step1_Input OD450 data'!G96","v":0},{"$f":"'Step1_Input OD450 data'!H96","v":0},{"$f":"'Step1_Input OD450 data'!I96","v":0},{"$f":"'Step1_Input OD450 data'!J96","v":0},{"$f":"'Step1_Input OD450 data'!K96","v":0},{"$f":"'Step1_Input OD450 data'!L96","v":0},{"$f":"'Step1_Input OD450 data'!M96","v":0},{"$f":"'Step1_Input OD450 data'!N96","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C97","v":0},{"$f":"'Step1_Input OD450 data'!D97","v":0},{"$f":"'Step1_Input OD450 data'!E97","v":0},{"$f":"'Step1_Input OD450 data'!F97","v":0},{"$f":"'Step1_Input OD450 data'!G97","v":0},{"$f":"'Step1_Input OD450 data'!H97","v":0},{"$f":"'Step1_Input OD450 data'!I97","v":0},{"$f":"'Step1_Input OD450 data'!J97","v":0},{"$f":"'Step1_Input OD450 data'!K97","v":0},{"$f":"'Step1_Input OD450 data'!L97","v":0},{"$f":"'Step1_Input OD450 data'!M97","v":0},{"$f":"'Step1_Input OD450 data'!N97","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C98","v":0},{"$f":"'Step1_Input OD450 data'!D98","v":0},{"$f":"'Step1_Input OD450 data'!E98","v":0},{"$f":"'Step1_Input OD450 data'!F98","v":0},{"$f":"'Step1_Input OD450 data'!G98","v":0},{"$f":"'Step1_Input OD450 data'!H98","v":0},{"$f":"'Step1_Input OD450 data'!I98","v":0},{"$f":"'Step1_Input OD450 data'!J98","v":0},{"$f":"'Step1_Input OD450 data'!K98","v":0},{"$f":"'Step1_Input OD450 data'!L98","v":0},{"$f":"'Step1_Input OD450 data'!M98","v":0},{"$f":"'Step1_Input OD450 data'!N98","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,55,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,56,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,57,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,58,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,59,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,60,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,61,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,62,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,63,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 8","index":10,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C102","v":0},{"$f":"'Step1_Input OD450 data'!D102","v":0},{"$f":"'Step1_Input OD450 data'!E102","v":0},{"$f":"'Step1_Input OD450 data'!F102","v":0},{"$f":"'Step1_Input OD450 data'!G102","v":0},{"$f":"'Step1_Input OD450 data'!H102","v":0},{"$f":"'Step1_Input OD450 data'!I102","v":0},{"$f":"'Step1_Input OD450 data'!J102","v":0},{"$f":"'Step1_Input OD450 data'!K102","v":0},{"$f":"'Step1_Input OD450 data'!L102","v":0},{"$f":"'Step1_Input OD450 data'!M102","v":0},{"$f":"'Step1_Input OD450 data'!N102","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C103","v":0},{"$f":"'Step1_Input OD450 data'!D103","v":0},{"$f":"'Step1_Input OD450 data'!E103","v":0},{"$f":"'Step1_Input OD450 data'!F103","v":0},{"$f":"'Step1_Input OD450 data'!G103","v":0},{"$f":"'Step1_Input OD450 data'!H103","v":0},{"$f":"'Step1_Input OD450 data'!I103","v":0},{"$f":"'Step1_Input OD450 data'!J103","v":0},{"$f":"'Step1_Input OD450 data'!K103","v":0},{"$f":"'Step1_Input OD450 data'!L103","v":0},{"$f":"'Step1_Input OD450 data'!M103","v":0},{"$f":"'Step1_Input OD450 data'!N103","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C104","v":0},{"$f":"'Step1_Input OD450 data'!D104","v":0},{"$f":"'Step1_Input OD450 data'!E104","v":0},{"$f":"'Step1_Input OD450 data'!F104","v":0},{"$f":"'Step1_Input OD450 data'!G104","v":0},{"$f":"'Step1_Input OD450 data'!H104","v":0},{"$f":"'Step1_Input OD450 data'!I104","v":0},{"$f":"'Step1_Input OD450 data'!J104","v":0},{"$f":"'Step1_Input OD450 data'!K104","v":0},{"$f":"'Step1_Input OD450 data'!L104","v":0},{"$f":"'Step1_Input OD450 data'!M104","v":0},{"$f":"'Step1_Input OD450 data'!N104","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C105","v":0},{"$f":"'Step1_Input OD450 data'!D105","v":0},{"$f":"'Step1_Input OD450 data'!E105","v":0},{"$f":"'Step1_Input OD450 data'!F105","v":0},{"$f":"'Step1_Input OD450 data'!G105","v":0},{"$f":"'Step1_Input OD450 data'!H105","v":0},{"$f":"'Step1_Input OD450 data'!I105","v":0},{"$f":"'Step1_Input OD450 data'!J105","v":0},{"$f":"'Step1_Input OD450 data'!K105","v":0},{"$f":"'Step1_Input OD450 data'!L105","v":0},{"$f":"'Step1_Input OD450 data'!M105","v":0},{"$f":"'Step1_Input OD450 data'!N105","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C106","v":0},{"$f":"'Step1_Input OD450 data'!D106","v":0},{"$f":"'Step1_Input OD450 data'!E106","v":0},{"$f":"'Step1_Input OD450 data'!F106","v":0},{"$f":"'Step1_Input OD450 data'!G106","v":0},{"$f":"'Step1_Input OD450 data'!H106","v":0},{"$f":"'Step1_Input OD450 data'!I106","v":0},{"$f":"'Step1_Input OD450 data'!J106","v":0},{"$f":"'Step1_Input OD450 data'!K106","v":0},{"$f":"'Step1_Input OD450 data'!L106","v":0},{"$f":"'Step1_Input OD450 data'!M106","v":0},{"$f":"'Step1_Input OD450 data'!N106","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C107","v":0},{"$f":"'Step1_Input OD450 data'!D107","v":0},{"$f":"'Step1_Input OD450 data'!E107","v":0},{"$f":"'Step1_Input OD450 data'!F107","v":0},{"$f":"'Step1_Input OD450 data'!G107","v":0},{"$f":"'Step1_Input OD450 data'!H107","v":0},{"$f":"'Step1_Input OD450 data'!I107","v":0},{"$f":"'Step1_Input OD450 data'!J107","v":0},{"$f":"'Step1_Input OD450 data'!K107","v":0},{"$f":"'Step1_Input OD450 data'!L107","v":0},{"$f":"'Step1_Input OD450 data'!M107","v":0},{"$f":"'Step1_Input OD450 data'!N107","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C108","v":0},{"$f":"'Step1_Input OD450 data'!D108","v":0},{"$f":"'Step1_Input OD450 data'!E108","v":0},{"$f":"'Step1_Input OD450 data'!F108","v":0},{"$f":"'Step1_Input OD450 data'!G108","v":0},{"$f":"'Step1_Input OD450 data'!H108","v":0},{"$f":"'Step1_Input OD450 data'!I108","v":0},{"$f":"'Step1_Input OD450 data'!J108","v":0},{"$f":"'Step1_Input OD450 data'!K108","v":0},{"$f":"'Step1_Input OD450 data'!L108","v":0},{"$f":"'Step1_Input OD450 data'!M108","v":0},{"$f":"'Step1_Input OD450 data'!N108","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C109","v":0},{"$f":"'Step1_Input OD450 data'!D109","v":0},{"$f":"'Step1_Input OD450 data'!E109","v":0},{"$f":"'Step1_Input OD450 data'!F109","v":0},{"$f":"'Step1_Input OD450 data'!G109","v":0},{"$f":"'Step1_Input OD450 data'!H109","v":0},{"$f":"'Step1_Input OD450 data'!I109","v":0},{"$f":"'Step1_Input OD450 data'!J109","v":0},{"$f":"'Step1_Input OD450 data'!K109","v":0},{"$f":"'Step1_Input OD450 data'!L109","v":0},{"$f":"'Step1_Input OD450 data'!M109","v":0},{"$f":"'Step1_Input OD450 data'!N109","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,64,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,65,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,66,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,67,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,68,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,69,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,70,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,71,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,72,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 9","index":11,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C113","v":0},{"$f":"'Step1_Input OD450 data'!D113","v":0},{"$f":"'Step1_Input OD450 data'!E113","v":0},{"$f":"'Step1_Input OD450 data'!F113","v":0},{"$f":"'Step1_Input OD450 data'!G113","v":0},{"$f":"'Step1_Input OD450 data'!H113","v":0},{"$f":"'Step1_Input OD450 data'!I113","v":0},{"$f":"'Step1_Input OD450 data'!J113","v":0},{"$f":"'Step1_Input OD450 data'!K113","v":0},{"$f":"'Step1_Input OD450 data'!L113","v":0},{"$f":"'Step1_Input OD450 data'!M113","v":0},{"$f":"'Step1_Input OD450 data'!N113","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C114","v":0},{"$f":"'Step1_Input OD450 data'!D114","v":0},{"$f":"'Step1_Input OD450 data'!E114","v":0},{"$f":"'Step1_Input OD450 data'!F114","v":0},{"$f":"'Step1_Input OD450 data'!G114","v":0},{"$f":"'Step1_Input OD450 data'!H114","v":0},{"$f":"'Step1_Input OD450 data'!I114","v":0},{"$f":"'Step1_Input OD450 data'!J114","v":0},{"$f":"'Step1_Input OD450 data'!K114","v":0},{"$f":"'Step1_Input OD450 data'!L114","v":0},{"$f":"'Step1_Input OD450 data'!M114","v":0},{"$f":"'Step1_Input OD450 data'!N114","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C115","v":0},{"$f":"'Step1_Input OD450 data'!D115","v":0},{"$f":"'Step1_Input OD450 data'!E115","v":0},{"$f":"'Step1_Input OD450 data'!F115","v":0},{"$f":"'Step1_Input OD450 data'!G115","v":0},{"$f":"'Step1_Input OD450 data'!H115","v":0},{"$f":"'Step1_Input OD450 data'!I115","v":0},{"$f":"'Step1_Input OD450 data'!J115","v":0},{"$f":"'Step1_Input OD450 data'!K115","v":0},{"$f":"'Step1_Input OD450 data'!L115","v":0},{"$f":"'Step1_Input OD450 data'!M115","v":0},{"$f":"'Step1_Input OD450 data'!N115","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C116","v":0},{"$f":"'Step1_Input OD450 data'!D116","v":0},{"$f":"'Step1_Input OD450 data'!E116","v":0},{"$f":"'Step1_Input OD450 data'!F116","v":0},{"$f":"'Step1_Input OD450 data'!G116","v":0},{"$f":"'Step1_Input OD450 data'!H116","v":0},{"$f":"'Step1_Input OD450 data'!I116","v":0},{"$f":"'Step1_Input OD450 data'!J116","v":0},{"$f":"'Step1_Input OD450 data'!K116","v":0},{"$f":"'Step1_Input OD450 data'!L116","v":0},{"$f":"'Step1_Input OD450 data'!M116","v":0},{"$f":"'Step1_Input OD450 data'!N116","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C117","v":0},{"$f":"'Step1_Input OD450 data'!D117","v":0},{"$f":"'Step1_Input OD450 data'!E117","v":0},{"$f":"'Step1_Input OD450 data'!F117","v":0},{"$f":"'Step1_Input OD450 data'!G117","v":0},{"$f":"'Step1_Input OD450 data'!H117","v":0},{"$f":"'Step1_Input OD450 data'!I117","v":0},{"$f":"'Step1_Input OD450 data'!J117","v":0},{"$f":"'Step1_Input OD450 data'!K117","v":0},{"$f":"'Step1_Input OD450 data'!L117","v":0},{"$f":"'Step1_Input OD450 data'!M117","v":0},{"$f":"'Step1_Input OD450 data'!N117","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C118","v":0},{"$f":"'Step1_Input OD450 data'!D118","v":0},{"$f":"'Step1_Input OD450 data'!E118","v":0},{"$f":"'Step1_Input OD450 data'!F118","v":0},{"$f":"'Step1_Input OD450 data'!G118","v":0},{"$f":"'Step1_Input OD450 data'!H118","v":0},{"$f":"'Step1_Input OD450 data'!I118","v":0},{"$f":"'Step1_Input OD450 data'!J118","v":0},{"$f":"'Step1_Input OD450 data'!K118","v":0},{"$f":"'Step1_Input OD450 data'!L118","v":0},{"$f":"'Step1_Input OD450 data'!M118","v":0},{"$f":"'Step1_Input OD450 data'!N118","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C119","v":0},{"$f":"'Step1_Input OD450 data'!D119","v":0},{"$f":"'Step1_Input OD450 data'!E119","v":0},{"$f":"'Step1_Input OD450 data'!F119","v":0},{"$f":"'Step1_Input OD450 data'!G119","v":0},{"$f":"'Step1_Input OD450 data'!H119","v":0},{"$f":"'Step1_Input OD450 data'!I119","v":0},{"$f":"'Step1_Input OD450 data'!J119","v":0},{"$f":"'Step1_Input OD450 data'!K119","v":0},{"$f":"'Step1_Input OD450 data'!L119","v":0},{"$f":"'Step1_Input OD450 data'!M119","v":0},{"$f":"'Step1_Input OD450 data'!N119","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C120","v":0},{"$f":"'Step1_Input OD450 data'!D120","v":0},{"$f":"'Step1_Input OD450 data'!E120","v":0},{"$f":"'Step1_Input OD450 data'!F120","v":0},{"$f":"'Step1_Input OD450 data'!G120","v":0},{"$f":"'Step1_Input OD450 data'!H120","v":0},{"$f":"'Step1_Input OD450 data'!I120","v":0},{"$f":"'Step1_Input OD450 data'!J120","v":0},{"$f":"'Step1_Input OD450 data'!K120","v":0},{"$f":"'Step1_Input OD450 data'!L120","v":0},{"$f":"'Step1_Input OD450 data'!M120","v":0},{"$f":"'Step1_Input OD450 data'!N120","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,73,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,74,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,75,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,76,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,77,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,78,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,79,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,80,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,81,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 10","index":12,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!C124","v":0},{"$f":"'Step1_Input OD450 data'!D124","v":0},{"$f":"'Step1_Input OD450 data'!E124","v":0},{"$f":"'Step1_Input OD450 data'!F124","v":0},{"$f":"'Step1_Input OD450 data'!G124","v":0},{"$f":"'Step1_Input OD450 data'!H124","v":0},{"$f":"'Step1_Input OD450 data'!I124","v":0},{"$f":"'Step1_Input OD450 data'!J124","v":0},{"$f":"'Step1_Input OD450 data'!K124","v":0},{"$f":"'Step1_Input OD450 data'!L124","v":0},{"$f":"'Step1_Input OD450 data'!M124","v":0},{"$f":"'Step1_Input OD450 data'!N124","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!C125","v":0},{"$f":"'Step1_Input OD450 data'!D125","v":0},{"$f":"'Step1_Input OD450 data'!E125","v":0},{"$f":"'Step1_Input OD450 data'!F125","v":0},{"$f":"'Step1_Input OD450 data'!G125","v":0},{"$f":"'Step1_Input OD450 data'!H125","v":0},{"$f":"'Step1_Input OD450 data'!I125","v":0},{"$f":"'Step1_Input OD450 data'!J125","v":0},{"$f":"'Step1_Input OD450 data'!K125","v":0},{"$f":"'Step1_Input OD450 data'!L125","v":0},{"$f":"'Step1_Input OD450 data'!M125","v":0},{"$f":"'Step1_Input OD450 data'!N125","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!C126","v":0},{"$f":"'Step1_Input OD450 data'!D126","v":0},{"$f":"'Step1_Input OD450 data'!E126","v":0},{"$f":"'Step1_Input OD450 data'!F126","v":0},{"$f":"'Step1_Input OD450 data'!G126","v":0},{"$f":"'Step1_Input OD450 data'!H126","v":0},{"$f":"'Step1_Input OD450 data'!I126","v":0},{"$f":"'Step1_Input OD450 data'!J126","v":0},{"$f":"'Step1_Input OD450 data'!K126","v":0},{"$f":"'Step1_Input OD450 data'!L126","v":0},{"$f":"'Step1_Input OD450 data'!M126","v":0},{"$f":"'Step1_Input OD450 data'!N126","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!C127","v":0},{"$f":"'Step1_Input OD450 data'!D127","v":0},{"$f":"'Step1_Input OD450 data'!E127","v":0},{"$f":"'Step1_Input OD450 data'!F127","v":0},{"$f":"'Step1_Input OD450 data'!G127","v":0},{"$f":"'Step1_Input OD450 data'!H127","v":0},{"$f":"'Step1_Input OD450 data'!I127","v":0},{"$f":"'Step1_Input OD450 data'!J127","v":0},{"$f":"'Step1_Input OD450 data'!K127","v":0},{"$f":"'Step1_Input OD450 data'!L127","v":0},{"$f":"'Step1_Input OD450 data'!M127","v":0},{"$f":"'Step1_Input OD450 data'!N127","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!C128","v":0},{"$f":"'Step1_Input OD450 data'!D128","v":0},{"$f":"'Step1_Input OD450 data'!E128","v":0},{"$f":"'Step1_Input OD450 data'!F128","v":0},{"$f":"'Step1_Input OD450 data'!G128","v":0},{"$f":"'Step1_Input OD450 data'!H128","v":0},{"$f":"'Step1_Input OD450 data'!I128","v":0},{"$f":"'Step1_Input OD450 data'!J128","v":0},{"$f":"'Step1_Input OD450 data'!K128","v":0},{"$f":"'Step1_Input OD450 data'!L128","v":0},{"$f":"'Step1_Input OD450 data'!M128","v":0},{"$f":"'Step1_Input OD450 data'!N128","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!C129","v":0},{"$f":"'Step1_Input OD450 data'!D129","v":0},{"$f":"'Step1_Input OD450 data'!E129","v":0},{"$f":"'Step1_Input OD450 data'!F129","v":0},{"$f":"'Step1_Input OD450 data'!G129","v":0},{"$f":"'Step1_Input OD450 data'!H129","v":0},{"$f":"'Step1_Input OD450 data'!I129","v":0},{"$f":"'Step1_Input OD450 data'!J129","v":0},{"$f":"'Step1_Input OD450 data'!K129","v":0},{"$f":"'Step1_Input OD450 data'!L129","v":0},{"$f":"'Step1_Input OD450 data'!M129","v":0},{"$f":"'Step1_Input OD450 data'!N129","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!C130","v":0},{"$f":"'Step1_Input OD450 data'!D130","v":0},{"$f":"'Step1_Input OD450 data'!E130","v":0},{"$f":"'Step1_Input OD450 data'!F130","v":0},{"$f":"'Step1_Input OD450 data'!G130","v":0},{"$f":"'Step1_Input OD450 data'!H130","v":0},{"$f":"'Step1_Input OD450 data'!I130","v":0},{"$f":"'Step1_Input OD450 data'!J130","v":0},{"$f":"'Step1_Input OD450 data'!K130","v":0},{"$f":"'Step1_Input OD450 data'!L130","v":0},{"$f":"'Step1_Input OD450 data'!M130","v":0},{"$f":"'Step1_Input OD450 data'!N130","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!C131","v":0},{"$f":"'Step1_Input OD450 data'!D131","v":0},{"$f":"'Step1_Input OD450 data'!E131","v":0},{"$f":"'Step1_Input OD450 data'!F131","v":0},{"$f":"'Step1_Input OD450 data'!G131","v":0},{"$f":"'Step1_Input OD450 data'!H131","v":0},{"$f":"'Step1_Input OD450 data'!I131","v":0},{"$f":"'Step1_Input OD450 data'!J131","v":0},{"$f":"'Step1_Input OD450 data'!K131","v":0},{"$f":"'Step1_Input OD450 data'!L131","v":0},{"$f":"'Step1_Input OD450 data'!M131","v":0},{"$f":"'Step1_Input OD450 data'!N131","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,82,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,83,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,84,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,85,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,86,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,87,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,88,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,89,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,90,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 11","index":13,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R25","v":0},{"$f":"'Step1_Input OD450 data'!S25","v":0},{"$f":"'Step1_Input OD450 data'!T25","v":0},{"$f":"'Step1_Input OD450 data'!U25","v":0},{"$f":"'Step1_Input OD450 data'!V25","v":0},{"$f":"'Step1_Input OD450 data'!W25","v":0},{"$f":"'Step1_Input OD450 data'!X25","v":0},{"$f":"'Step1_Input OD450 data'!Y25","v":0},{"$f":"'Step1_Input OD450 data'!Z25","v":0},{"$f":"'Step1_Input OD450 data'!AA25","v":0},{"$f":"'Step1_Input OD450 data'!AB25","v":0},{"$f":"'Step1_Input OD450 data'!AC25","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R26","v":0},{"$f":"'Step1_Input OD450 data'!S26","v":0},{"$f":"'Step1_Input OD450 data'!T26","v":0},{"$f":"'Step1_Input OD450 data'!U26","v":0},{"$f":"'Step1_Input OD450 data'!V26","v":0},{"$f":"'Step1_Input OD450 data'!W26","v":0},{"$f":"'Step1_Input OD450 data'!X26","v":0},{"$f":"'Step1_Input OD450 data'!Y26","v":0},{"$f":"'Step1_Input OD450 data'!Z26","v":0},{"$f":"'Step1_Input OD450 data'!AA26","v":0},{"$f":"'Step1_Input OD450 data'!AB26","v":0},{"$f":"'Step1_Input OD450 data'!AC26","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R27","v":0},{"$f":"'Step1_Input OD450 data'!S27","v":0},{"$f":"'Step1_Input OD450 data'!T27","v":0},{"$f":"'Step1_Input OD450 data'!U27","v":0},{"$f":"'Step1_Input OD450 data'!V27","v":0},{"$f":"'Step1_Input OD450 data'!W27","v":0},{"$f":"'Step1_Input OD450 data'!X27","v":0},{"$f":"'Step1_Input OD450 data'!Y27","v":0},{"$f":"'Step1_Input OD450 data'!Z27","v":0},{"$f":"'Step1_Input OD450 data'!AA27","v":0},{"$f":"'Step1_Input OD450 data'!AB27","v":0},{"$f":"'Step1_Input OD450 data'!AC27","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R28","v":0},{"$f":"'Step1_Input OD450 data'!S28","v":0},{"$f":"'Step1_Input OD450 data'!T28","v":0},{"$f":"'Step1_Input OD450 data'!U28","v":0},{"$f":"'Step1_Input OD450 data'!V28","v":0},{"$f":"'Step1_Input OD450 data'!W28","v":0},{"$f":"'Step1_Input OD450 data'!X28","v":0},{"$f":"'Step1_Input OD450 data'!Y28","v":0},{"$f":"'Step1_Input OD450 data'!Z28","v":0},{"$f":"'Step1_Input OD450 data'!AA28","v":0},{"$f":"'Step1_Input OD450 data'!AB28","v":0},{"$f":"'Step1_Input OD450 data'!AC28","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R29","v":0},{"$f":"'Step1_Input OD450 data'!S29","v":0},{"$f":"'Step1_Input OD450 data'!T29","v":0},{"$f":"'Step1_Input OD450 data'!U29","v":0},{"$f":"'Step1_Input OD450 data'!V29","v":0},{"$f":"'Step1_Input OD450 data'!W29","v":0},{"$f":"'Step1_Input OD450 data'!X29","v":0},{"$f":"'Step1_Input OD450 data'!Y29","v":0},{"$f":"'Step1_Input OD450 data'!Z29","v":0},{"$f":"'Step1_Input OD450 data'!AA29","v":0},{"$f":"'Step1_Input OD450 data'!AB29","v":0},{"$f":"'Step1_Input OD450 data'!AC29","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R30","v":0},{"$f":"'Step1_Input OD450 data'!S30","v":0},{"$f":"'Step1_Input OD450 data'!T30","v":0},{"$f":"'Step1_Input OD450 data'!U30","v":0},{"$f":"'Step1_Input OD450 data'!V30","v":0},{"$f":"'Step1_Input OD450 data'!W30","v":0},{"$f":"'Step1_Input OD450 data'!X30","v":0},{"$f":"'Step1_Input OD450 data'!Y30","v":0},{"$f":"'Step1_Input OD450 data'!Z30","v":0},{"$f":"'Step1_Input OD450 data'!AA30","v":0},{"$f":"'Step1_Input OD450 data'!AB30","v":0},{"$f":"'Step1_Input OD450 data'!AC30","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R31","v":0},{"$f":"'Step1_Input OD450 data'!S31","v":0},{"$f":"'Step1_Input OD450 data'!T31","v":0},{"$f":"'Step1_Input OD450 data'!U31","v":0},{"$f":"'Step1_Input OD450 data'!V31","v":0},{"$f":"'Step1_Input OD450 data'!W31","v":0},{"$f":"'Step1_Input OD450 data'!X31","v":0},{"$f":"'Step1_Input OD450 data'!Y31","v":0},{"$f":"'Step1_Input OD450 data'!Z31","v":0},{"$f":"'Step1_Input OD450 data'!AA31","v":0},{"$f":"'Step1_Input OD450 data'!AB31","v":0},{"$f":"'Step1_Input OD450 data'!AC31","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R32","v":0},{"$f":"'Step1_Input OD450 data'!S32","v":0},{"$f":"'Step1_Input OD450 data'!T32","v":0},{"$f":"'Step1_Input OD450 data'!U32","v":0},{"$f":"'Step1_Input OD450 data'!V32","v":0},{"$f":"'Step1_Input OD450 data'!W32","v":0},{"$f":"'Step1_Input OD450 data'!X32","v":0},{"$f":"'Step1_Input OD450 data'!Y32","v":0},{"$f":"'Step1_Input OD450 data'!Z32","v":0},{"$f":"'Step1_Input OD450 data'!AA32","v":0},{"$f":"'Step1_Input OD450 data'!AB32","v":0},{"$f":"'Step1_Input OD450 data'!AC32","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,91,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,92,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,93,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,94,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,95,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,96,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,97,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,98,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,99,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 12","index":14,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R36","v":0},{"$f":"'Step1_Input OD450 data'!S36","v":0},{"$f":"'Step1_Input OD450 data'!T36","v":0},{"$f":"'Step1_Input OD450 data'!U36","v":0},{"$f":"'Step1_Input OD450 data'!V36","v":0},{"$f":"'Step1_Input OD450 data'!W36","v":0},{"$f":"'Step1_Input OD450 data'!X36","v":0},{"$f":"'Step1_Input OD450 data'!Y36","v":0},{"$f":"'Step1_Input OD450 data'!Z36","v":0},{"$f":"'Step1_Input OD450 data'!AA36","v":0},{"$f":"'Step1_Input OD450 data'!AB36","v":0},{"$f":"'Step1_Input OD450 data'!AC36","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R37","v":0},{"$f":"'Step1_Input OD450 data'!S37","v":0},{"$f":"'Step1_Input OD450 data'!T37","v":0},{"$f":"'Step1_Input OD450 data'!U37","v":0},{"$f":"'Step1_Input OD450 data'!V37","v":0},{"$f":"'Step1_Input OD450 data'!W37","v":0},{"$f":"'Step1_Input OD450 data'!X37","v":0},{"$f":"'Step1_Input OD450 data'!Y37","v":0},{"$f":"'Step1_Input OD450 data'!Z37","v":0},{"$f":"'Step1_Input OD450 data'!AA37","v":0},{"$f":"'Step1_Input OD450 data'!AB37","v":0},{"$f":"'Step1_Input OD450 data'!AC37","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R38","v":0},{"$f":"'Step1_Input OD450 data'!S38","v":0},{"$f":"'Step1_Input OD450 data'!T38","v":0},{"$f":"'Step1_Input OD450 data'!U38","v":0},{"$f":"'Step1_Input OD450 data'!V38","v":0},{"$f":"'Step1_Input OD450 data'!W38","v":0},{"$f":"'Step1_Input OD450 data'!X38","v":0},{"$f":"'Step1_Input OD450 data'!Y38","v":0},{"$f":"'Step1_Input OD450 data'!Z38","v":0},{"$f":"'Step1_Input OD450 data'!AA38","v":0},{"$f":"'Step1_Input OD450 data'!AB38","v":0},{"$f":"'Step1_Input OD450 data'!AC38","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R39","v":0},{"$f":"'Step1_Input OD450 data'!S39","v":0},{"$f":"'Step1_Input OD450 data'!T39","v":0},{"$f":"'Step1_Input OD450 data'!U39","v":0},{"$f":"'Step1_Input OD450 data'!V39","v":0},{"$f":"'Step1_Input OD450 data'!W39","v":0},{"$f":"'Step1_Input OD450 data'!X39","v":0},{"$f":"'Step1_Input OD450 data'!Y39","v":0},{"$f":"'Step1_Input OD450 data'!Z39","v":0},{"$f":"'Step1_Input OD450 data'!AA39","v":0},{"$f":"'Step1_Input OD450 data'!AB39","v":0},{"$f":"'Step1_Input OD450 data'!AC39","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R40","v":0},{"$f":"'Step1_Input OD450 data'!S40","v":0},{"$f":"'Step1_Input OD450 data'!T40","v":0},{"$f":"'Step1_Input OD450 data'!U40","v":0},{"$f":"'Step1_Input OD450 data'!V40","v":0},{"$f":"'Step1_Input OD450 data'!W40","v":0},{"$f":"'Step1_Input OD450 data'!X40","v":0},{"$f":"'Step1_Input OD450 data'!Y40","v":0},{"$f":"'Step1_Input OD450 data'!Z40","v":0},{"$f":"'Step1_Input OD450 data'!AA40","v":0},{"$f":"'Step1_Input OD450 data'!AB40","v":0},{"$f":"'Step1_Input OD450 data'!AC40","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R41","v":0},{"$f":"'Step1_Input OD450 data'!S41","v":0},{"$f":"'Step1_Input OD450 data'!T41","v":0},{"$f":"'Step1_Input OD450 data'!U41","v":0},{"$f":"'Step1_Input OD450 data'!V41","v":0},{"$f":"'Step1_Input OD450 data'!W41","v":0},{"$f":"'Step1_Input OD450 data'!X41","v":0},{"$f":"'Step1_Input OD450 data'!Y41","v":0},{"$f":"'Step1_Input OD450 data'!Z41","v":0},{"$f":"'Step1_Input OD450 data'!AA41","v":0},{"$f":"'Step1_Input OD450 data'!AB41","v":0},{"$f":"'Step1_Input OD450 data'!AC41","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R42","v":0},{"$f":"'Step1_Input OD450 data'!S42","v":0},{"$f":"'Step1_Input OD450 data'!T42","v":0},{"$f":"'Step1_Input OD450 data'!U42","v":0},{"$f":"'Step1_Input OD450 data'!V42","v":0},{"$f":"'Step1_Input OD450 data'!W42","v":0},{"$f":"'Step1_Input OD450 data'!X42","v":0},{"$f":"'Step1_Input OD450 data'!Y42","v":0},{"$f":"'Step1_Input OD450 data'!Z42","v":0},{"$f":"'Step1_Input OD450 data'!AA42","v":0},{"$f":"'Step1_Input OD450 data'!AB42","v":0},{"$f":"'Step1_Input OD450 data'!AC42","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R43","v":0},{"$f":"'Step1_Input OD450 data'!S43","v":0},{"$f":"'Step1_Input OD450 data'!T43","v":0},{"$f":"'Step1_Input OD450 data'!U43","v":0},{"$f":"'Step1_Input OD450 data'!V43","v":0},{"$f":"'Step1_Input OD450 data'!W43","v":0},{"$f":"'Step1_Input OD450 data'!X43","v":0},{"$f":"'Step1_Input OD450 data'!Y43","v":0},{"$f":"'Step1_Input OD450 data'!Z43","v":0},{"$f":"'Step1_Input OD450 data'!AA43","v":0},{"$f":"'Step1_Input OD450 data'!AB43","v":0},{"$f":"'Step1_Input OD450 data'!AC43","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,100,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,101,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,102,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,103,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,104,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,105,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,106,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,107,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,108,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 13","index":15,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R47","v":0},{"$f":"'Step1_Input OD450 data'!S47","v":0},{"$f":"'Step1_Input OD450 data'!T47","v":0},{"$f":"'Step1_Input OD450 data'!U47","v":0},{"$f":"'Step1_Input OD450 data'!V47","v":0},{"$f":"'Step1_Input OD450 data'!W47","v":0},{"$f":"'Step1_Input OD450 data'!X47","v":0},{"$f":"'Step1_Input OD450 data'!Y47","v":0},{"$f":"'Step1_Input OD450 data'!Z47","v":0},{"$f":"'Step1_Input OD450 data'!AA47","v":0},{"$f":"'Step1_Input OD450 data'!AB47","v":0},{"$f":"'Step1_Input OD450 data'!AC47","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R48","v":0},{"$f":"'Step1_Input OD450 data'!S48","v":0},{"$f":"'Step1_Input OD450 data'!T48","v":0},{"$f":"'Step1_Input OD450 data'!U48","v":0},{"$f":"'Step1_Input OD450 data'!V48","v":0},{"$f":"'Step1_Input OD450 data'!W48","v":0},{"$f":"'Step1_Input OD450 data'!X48","v":0},{"$f":"'Step1_Input OD450 data'!Y48","v":0},{"$f":"'Step1_Input OD450 data'!Z48","v":0},{"$f":"'Step1_Input OD450 data'!AA48","v":0},{"$f":"'Step1_Input OD450 data'!AB48","v":0},{"$f":"'Step1_Input OD450 data'!AC48","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R49","v":0},{"$f":"'Step1_Input OD450 data'!S49","v":0},{"$f":"'Step1_Input OD450 data'!T49","v":0},{"$f":"'Step1_Input OD450 data'!U49","v":0},{"$f":"'Step1_Input OD450 data'!V49","v":0},{"$f":"'Step1_Input OD450 data'!W49","v":0},{"$f":"'Step1_Input OD450 data'!X49","v":0},{"$f":"'Step1_Input OD450 data'!Y49","v":0},{"$f":"'Step1_Input OD450 data'!Z49","v":0},{"$f":"'Step1_Input OD450 data'!AA49","v":0},{"$f":"'Step1_Input OD450 data'!AB49","v":0},{"$f":"'Step1_Input OD450 data'!AC49","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R50","v":0},{"$f":"'Step1_Input OD450 data'!S50","v":0},{"$f":"'Step1_Input OD450 data'!T50","v":0},{"$f":"'Step1_Input OD450 data'!U50","v":0},{"$f":"'Step1_Input OD450 data'!V50","v":0},{"$f":"'Step1_Input OD450 data'!W50","v":0},{"$f":"'Step1_Input OD450 data'!X50","v":0},{"$f":"'Step1_Input OD450 data'!Y50","v":0},{"$f":"'Step1_Input OD450 data'!Z50","v":0},{"$f":"'Step1_Input OD450 data'!AA50","v":0},{"$f":"'Step1_Input OD450 data'!AB50","v":0},{"$f":"'Step1_Input OD450 data'!AC50","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R51","v":0},{"$f":"'Step1_Input OD450 data'!S51","v":0},{"$f":"'Step1_Input OD450 data'!T51","v":0},{"$f":"'Step1_Input OD450 data'!U51","v":0},{"$f":"'Step1_Input OD450 data'!V51","v":0},{"$f":"'Step1_Input OD450 data'!W51","v":0},{"$f":"'Step1_Input OD450 data'!X51","v":0},{"$f":"'Step1_Input OD450 data'!Y51","v":0},{"$f":"'Step1_Input OD450 data'!Z51","v":0},{"$f":"'Step1_Input OD450 data'!AA51","v":0},{"$f":"'Step1_Input OD450 data'!AB51","v":0},{"$f":"'Step1_Input OD450 data'!AC51","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R52","v":0},{"$f":"'Step1_Input OD450 data'!S52","v":0},{"$f":"'Step1_Input OD450 data'!T52","v":0},{"$f":"'Step1_Input OD450 data'!U52","v":0},{"$f":"'Step1_Input OD450 data'!V52","v":0},{"$f":"'Step1_Input OD450 data'!W52","v":0},{"$f":"'Step1_Input OD450 data'!X52","v":0},{"$f":"'Step1_Input OD450 data'!Y52","v":0},{"$f":"'Step1_Input OD450 data'!Z52","v":0},{"$f":"'Step1_Input OD450 data'!AA52","v":0},{"$f":"'Step1_Input OD450 data'!AB52","v":0},{"$f":"'Step1_Input OD450 data'!AC52","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R53","v":0},{"$f":"'Step1_Input OD450 data'!S53","v":0},{"$f":"'Step1_Input OD450 data'!T53","v":0},{"$f":"'Step1_Input OD450 data'!U53","v":0},{"$f":"'Step1_Input OD450 data'!V53","v":0},{"$f":"'Step1_Input OD450 data'!W53","v":0},{"$f":"'Step1_Input OD450 data'!X53","v":0},{"$f":"'Step1_Input OD450 data'!Y53","v":0},{"$f":"'Step1_Input OD450 data'!Z53","v":0},{"$f":"'Step1_Input OD450 data'!AA53","v":0},{"$f":"'Step1_Input OD450 data'!AB53","v":0},{"$f":"'Step1_Input OD450 data'!AC53","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R54","v":0},{"$f":"'Step1_Input OD450 data'!S54","v":0},{"$f":"'Step1_Input OD450 data'!T54","v":0},{"$f":"'Step1_Input OD450 data'!U54","v":0},{"$f":"'Step1_Input OD450 data'!V54","v":0},{"$f":"'Step1_Input OD450 data'!W54","v":0},{"$f":"'Step1_Input OD450 data'!X54","v":0},{"$f":"'Step1_Input OD450 data'!Y54","v":0},{"$f":"'Step1_Input OD450 data'!Z54","v":0},{"$f":"'Step1_Input OD450 data'!AA54","v":0},{"$f":"'Step1_Input OD450 data'!AB54","v":0},{"$f":"'Step1_Input OD450 data'!AC54","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,109,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,110,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,111,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,112,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,113,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,114,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,115,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,116,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,117,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 14","index":16,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R58","v":0},{"$f":"'Step1_Input OD450 data'!S58","v":0},{"$f":"'Step1_Input OD450 data'!T58","v":0},{"$f":"'Step1_Input OD450 data'!U58","v":0},{"$f":"'Step1_Input OD450 data'!V58","v":0},{"$f":"'Step1_Input OD450 data'!W58","v":0},{"$f":"'Step1_Input OD450 data'!X58","v":0},{"$f":"'Step1_Input OD450 data'!Y58","v":0},{"$f":"'Step1_Input OD450 data'!Z58","v":0},{"$f":"'Step1_Input OD450 data'!AA58","v":0},{"$f":"'Step1_Input OD450 data'!AB58","v":0},{"$f":"'Step1_Input OD450 data'!AC58","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R59","v":0},{"$f":"'Step1_Input OD450 data'!S59","v":0},{"$f":"'Step1_Input OD450 data'!T59","v":0},{"$f":"'Step1_Input OD450 data'!U59","v":0},{"$f":"'Step1_Input OD450 data'!V59","v":0},{"$f":"'Step1_Input OD450 data'!W59","v":0},{"$f":"'Step1_Input OD450 data'!X59","v":0},{"$f":"'Step1_Input OD450 data'!Y59","v":0},{"$f":"'Step1_Input OD450 data'!Z59","v":0},{"$f":"'Step1_Input OD450 data'!AA59","v":0},{"$f":"'Step1_Input OD450 data'!AB59","v":0},{"$f":"'Step1_Input OD450 data'!AC59","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R60","v":0},{"$f":"'Step1_Input OD450 data'!S60","v":0},{"$f":"'Step1_Input OD450 data'!T60","v":0},{"$f":"'Step1_Input OD450 data'!U60","v":0},{"$f":"'Step1_Input OD450 data'!V60","v":0},{"$f":"'Step1_Input OD450 data'!W60","v":0},{"$f":"'Step1_Input OD450 data'!X60","v":0},{"$f":"'Step1_Input OD450 data'!Y60","v":0},{"$f":"'Step1_Input OD450 data'!Z60","v":0},{"$f":"'Step1_Input OD450 data'!AA60","v":0},{"$f":"'Step1_Input OD450 data'!AB60","v":0},{"$f":"'Step1_Input OD450 data'!AC60","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R61","v":0},{"$f":"'Step1_Input OD450 data'!S61","v":0},{"$f":"'Step1_Input OD450 data'!T61","v":0},{"$f":"'Step1_Input OD450 data'!U61","v":0},{"$f":"'Step1_Input OD450 data'!V61","v":0},{"$f":"'Step1_Input OD450 data'!W61","v":0},{"$f":"'Step1_Input OD450 data'!X61","v":0},{"$f":"'Step1_Input OD450 data'!Y61","v":0},{"$f":"'Step1_Input OD450 data'!Z61","v":0},{"$f":"'Step1_Input OD450 data'!AA61","v":0},{"$f":"'Step1_Input OD450 data'!AB61","v":0},{"$f":"'Step1_Input OD450 data'!AC61","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R62","v":0},{"$f":"'Step1_Input OD450 data'!S62","v":0},{"$f":"'Step1_Input OD450 data'!T62","v":0},{"$f":"'Step1_Input OD450 data'!U62","v":0},{"$f":"'Step1_Input OD450 data'!V62","v":0},{"$f":"'Step1_Input OD450 data'!W62","v":0},{"$f":"'Step1_Input OD450 data'!X62","v":0},{"$f":"'Step1_Input OD450 data'!Y62","v":0},{"$f":"'Step1_Input OD450 data'!Z62","v":0},{"$f":"'Step1_Input OD450 data'!AA62","v":0},{"$f":"'Step1_Input OD450 data'!AB62","v":0},{"$f":"'Step1_Input OD450 data'!AC62","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R63","v":0},{"$f":"'Step1_Input OD450 data'!S63","v":0},{"$f":"'Step1_Input OD450 data'!T63","v":0},{"$f":"'Step1_Input OD450 data'!U63","v":0},{"$f":"'Step1_Input OD450 data'!V63","v":0},{"$f":"'Step1_Input OD450 data'!W63","v":0},{"$f":"'Step1_Input OD450 data'!X63","v":0},{"$f":"'Step1_Input OD450 data'!Y63","v":0},{"$f":"'Step1_Input OD450 data'!Z63","v":0},{"$f":"'Step1_Input OD450 data'!AA63","v":0},{"$f":"'Step1_Input OD450 data'!AB63","v":0},{"$f":"'Step1_Input OD450 data'!AC63","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R64","v":0},{"$f":"'Step1_Input OD450 data'!S64","v":0},{"$f":"'Step1_Input OD450 data'!T64","v":0},{"$f":"'Step1_Input OD450 data'!U64","v":0},{"$f":"'Step1_Input OD450 data'!V64","v":0},{"$f":"'Step1_Input OD450 data'!W64","v":0},{"$f":"'Step1_Input OD450 data'!X64","v":0},{"$f":"'Step1_Input OD450 data'!Y64","v":0},{"$f":"'Step1_Input OD450 data'!Z64","v":0},{"$f":"'Step1_Input OD450 data'!AA64","v":0},{"$f":"'Step1_Input OD450 data'!AB64","v":0},{"$f":"'Step1_Input OD450 data'!AC64","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R65","v":0},{"$f":"'Step1_Input OD450 data'!S65","v":0},{"$f":"'Step1_Input OD450 data'!T65","v":0},{"$f":"'Step1_Input OD450 data'!U65","v":0},{"$f":"'Step1_Input OD450 data'!V65","v":0},{"$f":"'Step1_Input OD450 data'!W65","v":0},{"$f":"'Step1_Input OD450 data'!X65","v":0},{"$f":"'Step1_Input OD450 data'!Y65","v":0},{"$f":"'Step1_Input OD450 data'!Z65","v":0},{"$f":"'Step1_Input OD450 data'!AA65","v":0},{"$f":"'Step1_Input OD450 data'!AB65","v":0},{"$f":"'Step1_Input OD450 data'!AC65","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,118,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,119,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,120,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,121,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,122,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,123,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,124,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,125,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,126,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 15","index":17,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R69","v":0},{"$f":"'Step1_Input OD450 data'!S69","v":0},{"$f":"'Step1_Input OD450 data'!T69","v":0},{"$f":"'Step1_Input OD450 data'!U69","v":0},{"$f":"'Step1_Input OD450 data'!V69","v":0},{"$f":"'Step1_Input OD450 data'!W69","v":0},{"$f":"'Step1_Input OD450 data'!X69","v":0},{"$f":"'Step1_Input OD450 data'!Y69","v":0},{"$f":"'Step1_Input OD450 data'!Z69","v":0},{"$f":"'Step1_Input OD450 data'!AA69","v":0},{"$f":"'Step1_Input OD450 data'!AB69","v":0},{"$f":"'Step1_Input OD450 data'!AC69","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R70","v":0},{"$f":"'Step1_Input OD450 data'!S70","v":0},{"$f":"'Step1_Input OD450 data'!T70","v":0},{"$f":"'Step1_Input OD450 data'!U70","v":0},{"$f":"'Step1_Input OD450 data'!V70","v":0},{"$f":"'Step1_Input OD450 data'!W70","v":0},{"$f":"'Step1_Input OD450 data'!X70","v":0},{"$f":"'Step1_Input OD450 data'!Y70","v":0},{"$f":"'Step1_Input OD450 data'!Z70","v":0},{"$f":"'Step1_Input OD450 data'!AA70","v":0},{"$f":"'Step1_Input OD450 data'!AB70","v":0},{"$f":"'Step1_Input OD450 data'!AC70","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R71","v":0},{"$f":"'Step1_Input OD450 data'!S71","v":0},{"$f":"'Step1_Input OD450 data'!T71","v":0},{"$f":"'Step1_Input OD450 data'!U71","v":0},{"$f":"'Step1_Input OD450 data'!V71","v":0},{"$f":"'Step1_Input OD450 data'!W71","v":0},{"$f":"'Step1_Input OD450 data'!X71","v":0},{"$f":"'Step1_Input OD450 data'!Y71","v":0},{"$f":"'Step1_Input OD450 data'!Z71","v":0},{"$f":"'Step1_Input OD450 data'!AA71","v":0},{"$f":"'Step1_Input OD450 data'!AB71","v":0},{"$f":"'Step1_Input OD450 data'!AC71","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R72","v":0},{"$f":"'Step1_Input OD450 data'!S72","v":0},{"$f":"'Step1_Input OD450 data'!T72","v":0},{"$f":"'Step1_Input OD450 data'!U72","v":0},{"$f":"'Step1_Input OD450 data'!V72","v":0},{"$f":"'Step1_Input OD450 data'!W72","v":0},{"$f":"'Step1_Input OD450 data'!X72","v":0},{"$f":"'Step1_Input OD450 data'!Y72","v":0},{"$f":"'Step1_Input OD450 data'!Z72","v":0},{"$f":"'Step1_Input OD450 data'!AA72","v":0},{"$f":"'Step1_Input OD450 data'!AB72","v":0},{"$f":"'Step1_Input OD450 data'!AC72","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R73","v":0},{"$f":"'Step1_Input OD450 data'!S73","v":0},{"$f":"'Step1_Input OD450 data'!T73","v":0},{"$f":"'Step1_Input OD450 data'!U73","v":0},{"$f":"'Step1_Input OD450 data'!V73","v":0},{"$f":"'Step1_Input OD450 data'!W73","v":0},{"$f":"'Step1_Input OD450 data'!X73","v":0},{"$f":"'Step1_Input OD450 data'!Y73","v":0},{"$f":"'Step1_Input OD450 data'!Z73","v":0},{"$f":"'Step1_Input OD450 data'!AA73","v":0},{"$f":"'Step1_Input OD450 data'!AB73","v":0},{"$f":"'Step1_Input OD450 data'!AC73","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R74","v":0},{"$f":"'Step1_Input OD450 data'!S74","v":0},{"$f":"'Step1_Input OD450 data'!T74","v":0},{"$f":"'Step1_Input OD450 data'!U74","v":0},{"$f":"'Step1_Input OD450 data'!V74","v":0},{"$f":"'Step1_Input OD450 data'!W74","v":0},{"$f":"'Step1_Input OD450 data'!X74","v":0},{"$f":"'Step1_Input OD450 data'!Y74","v":0},{"$f":"'Step1_Input OD450 data'!Z74","v":0},{"$f":"'Step1_Input OD450 data'!AA74","v":0},{"$f":"'Step1_Input OD450 data'!AB74","v":0},{"$f":"'Step1_Input OD450 data'!AC74","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R75","v":0},{"$f":"'Step1_Input OD450 data'!S75","v":0},{"$f":"'Step1_Input OD450 data'!T75","v":0},{"$f":"'Step1_Input OD450 data'!U75","v":0},{"$f":"'Step1_Input OD450 data'!V75","v":0},{"$f":"'Step1_Input OD450 data'!W75","v":0},{"$f":"'Step1_Input OD450 data'!X75","v":0},{"$f":"'Step1_Input OD450 data'!Y75","v":0},{"$f":"'Step1_Input OD450 data'!Z75","v":0},{"$f":"'Step1_Input OD450 data'!AA75","v":0},{"$f":"'Step1_Input OD450 data'!AB75","v":0},{"$f":"'Step1_Input OD450 data'!AC75","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R76","v":0},{"$f":"'Step1_Input OD450 data'!S76","v":0},{"$f":"'Step1_Input OD450 data'!T76","v":0},{"$f":"'Step1_Input OD450 data'!U76","v":0},{"$f":"'Step1_Input OD450 data'!V76","v":0},{"$f":"'Step1_Input OD450 data'!W76","v":0},{"$f":"'Step1_Input OD450 data'!X76","v":0},{"$f":"'Step1_Input OD450 data'!Y76","v":0},{"$f":"'Step1_Input OD450 data'!Z76","v":0},{"$f":"'Step1_Input OD450 data'!AA76","v":0},{"$f":"'Step1_Input OD450 data'!AB76","v":0},{"$f":"'Step1_Input OD450 data'!AC76","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,127,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,128,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,129,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,130,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,131,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,132,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,133,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,134,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,135,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 16","index":18,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R80","v":0},{"$f":"'Step1_Input OD450 data'!S80","v":0},{"$f":"'Step1_Input OD450 data'!T80","v":0},{"$f":"'Step1_Input OD450 data'!U80","v":0},{"$f":"'Step1_Input OD450 data'!V80","v":0},{"$f":"'Step1_Input OD450 data'!W80","v":0},{"$f":"'Step1_Input OD450 data'!X80","v":0},{"$f":"'Step1_Input OD450 data'!Y80","v":0},{"$f":"'Step1_Input OD450 data'!Z80","v":0},{"$f":"'Step1_Input OD450 data'!AA80","v":0},{"$f":"'Step1_Input OD450 data'!AB80","v":0},{"$f":"'Step1_Input OD450 data'!AC80","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R81","v":0},{"$f":"'Step1_Input OD450 data'!S81","v":0},{"$f":"'Step1_Input OD450 data'!T81","v":0},{"$f":"'Step1_Input OD450 data'!U81","v":0},{"$f":"'Step1_Input OD450 data'!V81","v":0},{"$f":"'Step1_Input OD450 data'!W81","v":0},{"$f":"'Step1_Input OD450 data'!X81","v":0},{"$f":"'Step1_Input OD450 data'!Y81","v":0},{"$f":"'Step1_Input OD450 data'!Z81","v":0},{"$f":"'Step1_Input OD450 data'!AA81","v":0},{"$f":"'Step1_Input OD450 data'!AB81","v":0},{"$f":"'Step1_Input OD450 data'!AC81","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R82","v":0},{"$f":"'Step1_Input OD450 data'!S82","v":0},{"$f":"'Step1_Input OD450 data'!T82","v":0},{"$f":"'Step1_Input OD450 data'!U82","v":0},{"$f":"'Step1_Input OD450 data'!V82","v":0},{"$f":"'Step1_Input OD450 data'!W82","v":0},{"$f":"'Step1_Input OD450 data'!X82","v":0},{"$f":"'Step1_Input OD450 data'!Y82","v":0},{"$f":"'Step1_Input OD450 data'!Z82","v":0},{"$f":"'Step1_Input OD450 data'!AA82","v":0},{"$f":"'Step1_Input OD450 data'!AB82","v":0},{"$f":"'Step1_Input OD450 data'!AC82","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R83","v":0},{"$f":"'Step1_Input OD450 data'!S83","v":0},{"$f":"'Step1_Input OD450 data'!T83","v":0},{"$f":"'Step1_Input OD450 data'!U83","v":0},{"$f":"'Step1_Input OD450 data'!V83","v":0},{"$f":"'Step1_Input OD450 data'!W83","v":0},{"$f":"'Step1_Input OD450 data'!X83","v":0},{"$f":"'Step1_Input OD450 data'!Y83","v":0},{"$f":"'Step1_Input OD450 data'!Z83","v":0},{"$f":"'Step1_Input OD450 data'!AA83","v":0},{"$f":"'Step1_Input OD450 data'!AB83","v":0},{"$f":"'Step1_Input OD450 data'!AC83","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R84","v":0},{"$f":"'Step1_Input OD450 data'!S84","v":0},{"$f":"'Step1_Input OD450 data'!T84","v":0},{"$f":"'Step1_Input OD450 data'!U84","v":0},{"$f":"'Step1_Input OD450 data'!V84","v":0},{"$f":"'Step1_Input OD450 data'!W84","v":0},{"$f":"'Step1_Input OD450 data'!X84","v":0},{"$f":"'Step1_Input OD450 data'!Y84","v":0},{"$f":"'Step1_Input OD450 data'!Z84","v":0},{"$f":"'Step1_Input OD450 data'!AA84","v":0},{"$f":"'Step1_Input OD450 data'!AB84","v":0},{"$f":"'Step1_Input OD450 data'!AC84","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R85","v":0},{"$f":"'Step1_Input OD450 data'!S85","v":0},{"$f":"'Step1_Input OD450 data'!T85","v":0},{"$f":"'Step1_Input OD450 data'!U85","v":0},{"$f":"'Step1_Input OD450 data'!V85","v":0},{"$f":"'Step1_Input OD450 data'!W85","v":0},{"$f":"'Step1_Input OD450 data'!X85","v":0},{"$f":"'Step1_Input OD450 data'!Y85","v":0},{"$f":"'Step1_Input OD450 data'!Z85","v":0},{"$f":"'Step1_Input OD450 data'!AA85","v":0},{"$f":"'Step1_Input OD450 data'!AB85","v":0},{"$f":"'Step1_Input OD450 data'!AC85","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R86","v":0},{"$f":"'Step1_Input OD450 data'!S86","v":0},{"$f":"'Step1_Input OD450 data'!T86","v":0},{"$f":"'Step1_Input OD450 data'!U86","v":0},{"$f":"'Step1_Input OD450 data'!V86","v":0},{"$f":"'Step1_Input OD450 data'!W86","v":0},{"$f":"'Step1_Input OD450 data'!X86","v":0},{"$f":"'Step1_Input OD450 data'!Y86","v":0},{"$f":"'Step1_Input OD450 data'!Z86","v":0},{"$f":"'Step1_Input OD450 data'!AA86","v":0},{"$f":"'Step1_Input OD450 data'!AB86","v":0},{"$f":"'Step1_Input OD450 data'!AC86","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R87","v":0},{"$f":"'Step1_Input OD450 data'!S87","v":0},{"$f":"'Step1_Input OD450 data'!T87","v":0},{"$f":"'Step1_Input OD450 data'!U87","v":0},{"$f":"'Step1_Input OD450 data'!V87","v":0},{"$f":"'Step1_Input OD450 data'!W87","v":0},{"$f":"'Step1_Input OD450 data'!X87","v":0},{"$f":"'Step1_Input OD450 data'!Y87","v":0},{"$f":"'Step1_Input OD450 data'!Z87","v":0},{"$f":"'Step1_Input OD450 data'!AA87","v":0},{"$f":"'Step1_Input OD450 data'!AB87","v":0},{"$f":"'Step1_Input OD450 data'!AC87","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,136,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,137,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,138,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,139,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,140,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,141,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,142,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,143,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,144,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 17","index":19,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R91","v":0},{"$f":"'Step1_Input OD450 data'!S91","v":0},{"$f":"'Step1_Input OD450 data'!T91","v":0},{"$f":"'Step1_Input OD450 data'!U91","v":0},{"$f":"'Step1_Input OD450 data'!V91","v":0},{"$f":"'Step1_Input OD450 data'!W91","v":0},{"$f":"'Step1_Input OD450 data'!X91","v":0},{"$f":"'Step1_Input OD450 data'!Y91","v":0},{"$f":"'Step1_Input OD450 data'!Z91","v":0},{"$f":"'Step1_Input OD450 data'!AA91","v":0},{"$f":"'Step1_Input OD450 data'!AB91","v":0},{"$f":"'Step1_Input OD450 data'!AC91","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R92","v":0},{"$f":"'Step1_Input OD450 data'!S92","v":0},{"$f":"'Step1_Input OD450 data'!T92","v":0},{"$f":"'Step1_Input OD450 data'!U92","v":0},{"$f":"'Step1_Input OD450 data'!V92","v":0},{"$f":"'Step1_Input OD450 data'!W92","v":0},{"$f":"'Step1_Input OD450 data'!X92","v":0},{"$f":"'Step1_Input OD450 data'!Y92","v":0},{"$f":"'Step1_Input OD450 data'!Z92","v":0},{"$f":"'Step1_Input OD450 data'!AA92","v":0},{"$f":"'Step1_Input OD450 data'!AB92","v":0},{"$f":"'Step1_Input OD450 data'!AC92","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R93","v":0},{"$f":"'Step1_Input OD450 data'!S93","v":0},{"$f":"'Step1_Input OD450 data'!T93","v":0},{"$f":"'Step1_Input OD450 data'!U93","v":0},{"$f":"'Step1_Input OD450 data'!V93","v":0},{"$f":"'Step1_Input OD450 data'!W93","v":0},{"$f":"'Step1_Input OD450 data'!X93","v":0},{"$f":"'Step1_Input OD450 data'!Y93","v":0},{"$f":"'Step1_Input OD450 data'!Z93","v":0},{"$f":"'Step1_Input OD450 data'!AA93","v":0},{"$f":"'Step1_Input OD450 data'!AB93","v":0},{"$f":"'Step1_Input OD450 data'!AC93","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R94","v":0},{"$f":"'Step1_Input OD450 data'!S94","v":0},{"$f":"'Step1_Input OD450 data'!T94","v":0},{"$f":"'Step1_Input OD450 data'!U94","v":0},{"$f":"'Step1_Input OD450 data'!V94","v":0},{"$f":"'Step1_Input OD450 data'!W94","v":0},{"$f":"'Step1_Input OD450 data'!X94","v":0},{"$f":"'Step1_Input OD450 data'!Y94","v":0},{"$f":"'Step1_Input OD450 data'!Z94","v":0},{"$f":"'Step1_Input OD450 data'!AA94","v":0},{"$f":"'Step1_Input OD450 data'!AB94","v":0},{"$f":"'Step1_Input OD450 data'!AC94","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R95","v":0},{"$f":"'Step1_Input OD450 data'!S95","v":0},{"$f":"'Step1_Input OD450 data'!T95","v":0},{"$f":"'Step1_Input OD450 data'!U95","v":0},{"$f":"'Step1_Input OD450 data'!V95","v":0},{"$f":"'Step1_Input OD450 data'!W95","v":0},{"$f":"'Step1_Input OD450 data'!X95","v":0},{"$f":"'Step1_Input OD450 data'!Y95","v":0},{"$f":"'Step1_Input OD450 data'!Z95","v":0},{"$f":"'Step1_Input OD450 data'!AA95","v":0},{"$f":"'Step1_Input OD450 data'!AB95","v":0},{"$f":"'Step1_Input OD450 data'!AC95","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R96","v":0},{"$f":"'Step1_Input OD450 data'!S96","v":0},{"$f":"'Step1_Input OD450 data'!T96","v":0},{"$f":"'Step1_Input OD450 data'!U96","v":0},{"$f":"'Step1_Input OD450 data'!V96","v":0},{"$f":"'Step1_Input OD450 data'!W96","v":0},{"$f":"'Step1_Input OD450 data'!X96","v":0},{"$f":"'Step1_Input OD450 data'!Y96","v":0},{"$f":"'Step1_Input OD450 data'!Z96","v":0},{"$f":"'Step1_Input OD450 data'!AA96","v":0},{"$f":"'Step1_Input OD450 data'!AB96","v":0},{"$f":"'Step1_Input OD450 data'!AC96","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R97","v":0},{"$f":"'Step1_Input OD450 data'!S97","v":0},{"$f":"'Step1_Input OD450 data'!T97","v":0},{"$f":"'Step1_Input OD450 data'!U97","v":0},{"$f":"'Step1_Input OD450 data'!V97","v":0},{"$f":"'Step1_Input OD450 data'!W97","v":0},{"$f":"'Step1_Input OD450 data'!X97","v":0},{"$f":"'Step1_Input OD450 data'!Y97","v":0},{"$f":"'Step1_Input OD450 data'!Z97","v":0},{"$f":"'Step1_Input OD450 data'!AA97","v":0},{"$f":"'Step1_Input OD450 data'!AB97","v":0},{"$f":"'Step1_Input OD450 data'!AC97","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R98","v":0},{"$f":"'Step1_Input OD450 data'!S98","v":0},{"$f":"'Step1_Input OD450 data'!T98","v":0},{"$f":"'Step1_Input OD450 data'!U98","v":0},{"$f":"'Step1_Input OD450 data'!V98","v":0},{"$f":"'Step1_Input OD450 data'!W98","v":0},{"$f":"'Step1_Input OD450 data'!X98","v":0},{"$f":"'Step1_Input OD450 data'!Y98","v":0},{"$f":"'Step1_Input OD450 data'!Z98","v":0},{"$f":"'Step1_Input OD450 data'!AA98","v":0},{"$f":"'Step1_Input OD450 data'!AB98","v":0},{"$f":"'Step1_Input OD450 data'!AC98","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,145,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,146,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,147,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,148,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,149,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,150,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,151,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,152,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,153,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 18","index":20,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R102","v":0},{"$f":"'Step1_Input OD450 data'!S102","v":0},{"$f":"'Step1_Input OD450 data'!T102","v":0},{"$f":"'Step1_Input OD450 data'!U102","v":0},{"$f":"'Step1_Input OD450 data'!V102","v":0},{"$f":"'Step1_Input OD450 data'!W102","v":0},{"$f":"'Step1_Input OD450 data'!X102","v":0},{"$f":"'Step1_Input OD450 data'!Y102","v":0},{"$f":"'Step1_Input OD450 data'!Z102","v":0},{"$f":"'Step1_Input OD450 data'!AA102","v":0},{"$f":"'Step1_Input OD450 data'!AB102","v":0},{"$f":"'Step1_Input OD450 data'!AC102","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R103","v":0},{"$f":"'Step1_Input OD450 data'!S103","v":0},{"$f":"'Step1_Input OD450 data'!T103","v":0},{"$f":"'Step1_Input OD450 data'!U103","v":0},{"$f":"'Step1_Input OD450 data'!V103","v":0},{"$f":"'Step1_Input OD450 data'!W103","v":0},{"$f":"'Step1_Input OD450 data'!X103","v":0},{"$f":"'Step1_Input OD450 data'!Y103","v":0},{"$f":"'Step1_Input OD450 data'!Z103","v":0},{"$f":"'Step1_Input OD450 data'!AA103","v":0},{"$f":"'Step1_Input OD450 data'!AB103","v":0},{"$f":"'Step1_Input OD450 data'!AC103","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R104","v":0},{"$f":"'Step1_Input OD450 data'!S104","v":0},{"$f":"'Step1_Input OD450 data'!T104","v":0},{"$f":"'Step1_Input OD450 data'!U104","v":0},{"$f":"'Step1_Input OD450 data'!V104","v":0},{"$f":"'Step1_Input OD450 data'!W104","v":0},{"$f":"'Step1_Input OD450 data'!X104","v":0},{"$f":"'Step1_Input OD450 data'!Y104","v":0},{"$f":"'Step1_Input OD450 data'!Z104","v":0},{"$f":"'Step1_Input OD450 data'!AA104","v":0},{"$f":"'Step1_Input OD450 data'!AB104","v":0},{"$f":"'Step1_Input OD450 data'!AC104","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R105","v":0},{"$f":"'Step1_Input OD450 data'!S105","v":0},{"$f":"'Step1_Input OD450 data'!T105","v":0},{"$f":"'Step1_Input OD450 data'!U105","v":0},{"$f":"'Step1_Input OD450 data'!V105","v":0},{"$f":"'Step1_Input OD450 data'!W105","v":0},{"$f":"'Step1_Input OD450 data'!X105","v":0},{"$f":"'Step1_Input OD450 data'!Y105","v":0},{"$f":"'Step1_Input OD450 data'!Z105","v":0},{"$f":"'Step1_Input OD450 data'!AA105","v":0},{"$f":"'Step1_Input OD450 data'!AB105","v":0},{"$f":"'Step1_Input OD450 data'!AC105","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R106","v":0},{"$f":"'Step1_Input OD450 data'!S106","v":0},{"$f":"'Step1_Input OD450 data'!T106","v":0},{"$f":"'Step1_Input OD450 data'!U106","v":0},{"$f":"'Step1_Input OD450 data'!V106","v":0},{"$f":"'Step1_Input OD450 data'!W106","v":0},{"$f":"'Step1_Input OD450 data'!X106","v":0},{"$f":"'Step1_Input OD450 data'!Y106","v":0},{"$f":"'Step1_Input OD450 data'!Z106","v":0},{"$f":"'Step1_Input OD450 data'!AA106","v":0},{"$f":"'Step1_Input OD450 data'!AB106","v":0},{"$f":"'Step1_Input OD450 data'!AC106","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R107","v":0},{"$f":"'Step1_Input OD450 data'!S107","v":0},{"$f":"'Step1_Input OD450 data'!T107","v":0},{"$f":"'Step1_Input OD450 data'!U107","v":0},{"$f":"'Step1_Input OD450 data'!V107","v":0},{"$f":"'Step1_Input OD450 data'!W107","v":0},{"$f":"'Step1_Input OD450 data'!X107","v":0},{"$f":"'Step1_Input OD450 data'!Y107","v":0},{"$f":"'Step1_Input OD450 data'!Z107","v":0},{"$f":"'Step1_Input OD450 data'!AA107","v":0},{"$f":"'Step1_Input OD450 data'!AB107","v":0},{"$f":"'Step1_Input OD450 data'!AC107","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R108","v":0},{"$f":"'Step1_Input OD450 data'!S108","v":0},{"$f":"'Step1_Input OD450 data'!T108","v":0},{"$f":"'Step1_Input OD450 data'!U108","v":0},{"$f":"'Step1_Input OD450 data'!V108","v":0},{"$f":"'Step1_Input OD450 data'!W108","v":0},{"$f":"'Step1_Input OD450 data'!X108","v":0},{"$f":"'Step1_Input OD450 data'!Y108","v":0},{"$f":"'Step1_Input OD450 data'!Z108","v":0},{"$f":"'Step1_Input OD450 data'!AA108","v":0},{"$f":"'Step1_Input OD450 data'!AB108","v":0},{"$f":"'Step1_Input OD450 data'!AC108","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R109","v":0},{"$f":"'Step1_Input OD450 data'!S109","v":0},{"$f":"'Step1_Input OD450 data'!T109","v":0},{"$f":"'Step1_Input OD450 data'!U109","v":0},{"$f":"'Step1_Input OD450 data'!V109","v":0},{"$f":"'Step1_Input OD450 data'!W109","v":0},{"$f":"'Step1_Input OD450 data'!X109","v":0},{"$f":"'Step1_Input OD450 data'!Y109","v":0},{"$f":"'Step1_Input OD450 data'!Z109","v":0},{"$f":"'Step1_Input OD450 data'!AA109","v":0},{"$f":"'Step1_Input OD450 data'!AB109","v":0},{"$f":"'Step1_Input OD450 data'!AC109","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,154,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,155,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,156,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,157,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,158,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,159,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,160,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,161,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,162,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 19","index":21,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R113","v":0},{"$f":"'Step1_Input OD450 data'!S113","v":0},{"$f":"'Step1_Input OD450 data'!T113","v":0},{"$f":"'Step1_Input OD450 data'!U113","v":0},{"$f":"'Step1_Input OD450 data'!V113","v":0},{"$f":"'Step1_Input OD450 data'!W113","v":0},{"$f":"'Step1_Input OD450 data'!X113","v":0},{"$f":"'Step1_Input OD450 data'!Y113","v":0},{"$f":"'Step1_Input OD450 data'!Z113","v":0},{"$f":"'Step1_Input OD450 data'!AA113","v":0},{"$f":"'Step1_Input OD450 data'!AB113","v":0},{"$f":"'Step1_Input OD450 data'!AC113","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R114","v":0},{"$f":"'Step1_Input OD450 data'!S114","v":0},{"$f":"'Step1_Input OD450 data'!T114","v":0},{"$f":"'Step1_Input OD450 data'!U114","v":0},{"$f":"'Step1_Input OD450 data'!V114","v":0},{"$f":"'Step1_Input OD450 data'!W114","v":0},{"$f":"'Step1_Input OD450 data'!X114","v":0},{"$f":"'Step1_Input OD450 data'!Y114","v":0},{"$f":"'Step1_Input OD450 data'!Z114","v":0},{"$f":"'Step1_Input OD450 data'!AA114","v":0},{"$f":"'Step1_Input OD450 data'!AB114","v":0},{"$f":"'Step1_Input OD450 data'!AC114","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R115","v":0},{"$f":"'Step1_Input OD450 data'!S115","v":0},{"$f":"'Step1_Input OD450 data'!T115","v":0},{"$f":"'Step1_Input OD450 data'!U115","v":0},{"$f":"'Step1_Input OD450 data'!V115","v":0},{"$f":"'Step1_Input OD450 data'!W115","v":0},{"$f":"'Step1_Input OD450 data'!X115","v":0},{"$f":"'Step1_Input OD450 data'!Y115","v":0},{"$f":"'Step1_Input OD450 data'!Z115","v":0},{"$f":"'Step1_Input OD450 data'!AA115","v":0},{"$f":"'Step1_Input OD450 data'!AB115","v":0},{"$f":"'Step1_Input OD450 data'!AC115","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R116","v":0},{"$f":"'Step1_Input OD450 data'!S116","v":0},{"$f":"'Step1_Input OD450 data'!T116","v":0},{"$f":"'Step1_Input OD450 data'!U116","v":0},{"$f":"'Step1_Input OD450 data'!V116","v":0},{"$f":"'Step1_Input OD450 data'!W116","v":0},{"$f":"'Step1_Input OD450 data'!X116","v":0},{"$f":"'Step1_Input OD450 data'!Y116","v":0},{"$f":"'Step1_Input OD450 data'!Z116","v":0},{"$f":"'Step1_Input OD450 data'!AA116","v":0},{"$f":"'Step1_Input OD450 data'!AB116","v":0},{"$f":"'Step1_Input OD450 data'!AC116","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R117","v":0},{"$f":"'Step1_Input OD450 data'!S117","v":0},{"$f":"'Step1_Input OD450 data'!T117","v":0},{"$f":"'Step1_Input OD450 data'!U117","v":0},{"$f":"'Step1_Input OD450 data'!V117","v":0},{"$f":"'Step1_Input OD450 data'!W117","v":0},{"$f":"'Step1_Input OD450 data'!X117","v":0},{"$f":"'Step1_Input OD450 data'!Y117","v":0},{"$f":"'Step1_Input OD450 data'!Z117","v":0},{"$f":"'Step1_Input OD450 data'!AA117","v":0},{"$f":"'Step1_Input OD450 data'!AB117","v":0},{"$f":"'Step1_Input OD450 data'!AC117","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R118","v":0},{"$f":"'Step1_Input OD450 data'!S118","v":0},{"$f":"'Step1_Input OD450 data'!T118","v":0},{"$f":"'Step1_Input OD450 data'!U118","v":0},{"$f":"'Step1_Input OD450 data'!V118","v":0},{"$f":"'Step1_Input OD450 data'!W118","v":0},{"$f":"'Step1_Input OD450 data'!X118","v":0},{"$f":"'Step1_Input OD450 data'!Y118","v":0},{"$f":"'Step1_Input OD450 data'!Z118","v":0},{"$f":"'Step1_Input OD450 data'!AA118","v":0},{"$f":"'Step1_Input OD450 data'!AB118","v":0},{"$f":"'Step1_Input OD450 data'!AC118","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R119","v":0},{"$f":"'Step1_Input OD450 data'!S119","v":0},{"$f":"'Step1_Input OD450 data'!T119","v":0},{"$f":"'Step1_Input OD450 data'!U119","v":0},{"$f":"'Step1_Input OD450 data'!V119","v":0},{"$f":"'Step1_Input OD450 data'!W119","v":0},{"$f":"'Step1_Input OD450 data'!X119","v":0},{"$f":"'Step1_Input OD450 data'!Y119","v":0},{"$f":"'Step1_Input OD450 data'!Z119","v":0},{"$f":"'Step1_Input OD450 data'!AA119","v":0},{"$f":"'Step1_Input OD450 data'!AB119","v":0},{"$f":"'Step1_Input OD450 data'!AC119","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R120","v":0},{"$f":"'Step1_Input OD450 data'!S120","v":0},{"$f":"'Step1_Input OD450 data'!T120","v":0},{"$f":"'Step1_Input OD450 data'!U120","v":0},{"$f":"'Step1_Input OD450 data'!V120","v":0},{"$f":"'Step1_Input OD450 data'!W120","v":0},{"$f":"'Step1_Input OD450 data'!X120","v":0},{"$f":"'Step1_Input OD450 data'!Y120","v":0},{"$f":"'Step1_Input OD450 data'!Z120","v":0},{"$f":"'Step1_Input OD450 data'!AA120","v":0},{"$f":"'Step1_Input OD450 data'!AB120","v":0},{"$f":"'Step1_Input OD450 data'!AC120","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,163,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,164,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,165,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,166,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,167,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,168,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,169,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,170,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,171,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 20","index":22,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!R124","v":0},{"$f":"'Step1_Input OD450 data'!S124","v":0},{"$f":"'Step1_Input OD450 data'!T124","v":0},{"$f":"'Step1_Input OD450 data'!U124","v":0},{"$f":"'Step1_Input OD450 data'!V124","v":0},{"$f":"'Step1_Input OD450 data'!W124","v":0},{"$f":"'Step1_Input OD450 data'!X124","v":0},{"$f":"'Step1_Input OD450 data'!Y124","v":0},{"$f":"'Step1_Input OD450 data'!Z124","v":0},{"$f":"'Step1_Input OD450 data'!AA124","v":0},{"$f":"'Step1_Input OD450 data'!AB124","v":0},{"$f":"'Step1_Input OD450 data'!AC124","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!R125","v":0},{"$f":"'Step1_Input OD450 data'!S125","v":0},{"$f":"'Step1_Input OD450 data'!T125","v":0},{"$f":"'Step1_Input OD450 data'!U125","v":0},{"$f":"'Step1_Input OD450 data'!V125","v":0},{"$f":"'Step1_Input OD450 data'!W125","v":0},{"$f":"'Step1_Input OD450 data'!X125","v":0},{"$f":"'Step1_Input OD450 data'!Y125","v":0},{"$f":"'Step1_Input OD450 data'!Z125","v":0},{"$f":"'Step1_Input OD450 data'!AA125","v":0},{"$f":"'Step1_Input OD450 data'!AB125","v":0},{"$f":"'Step1_Input OD450 data'!AC125","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!R126","v":0},{"$f":"'Step1_Input OD450 data'!S126","v":0},{"$f":"'Step1_Input OD450 data'!T126","v":0},{"$f":"'Step1_Input OD450 data'!U126","v":0},{"$f":"'Step1_Input OD450 data'!V126","v":0},{"$f":"'Step1_Input OD450 data'!W126","v":0},{"$f":"'Step1_Input OD450 data'!X126","v":0},{"$f":"'Step1_Input OD450 data'!Y126","v":0},{"$f":"'Step1_Input OD450 data'!Z126","v":0},{"$f":"'Step1_Input OD450 data'!AA126","v":0},{"$f":"'Step1_Input OD450 data'!AB126","v":0},{"$f":"'Step1_Input OD450 data'!AC126","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!R127","v":0},{"$f":"'Step1_Input OD450 data'!S127","v":0},{"$f":"'Step1_Input OD450 data'!T127","v":0},{"$f":"'Step1_Input OD450 data'!U127","v":0},{"$f":"'Step1_Input OD450 data'!V127","v":0},{"$f":"'Step1_Input OD450 data'!W127","v":0},{"$f":"'Step1_Input OD450 data'!X127","v":0},{"$f":"'Step1_Input OD450 data'!Y127","v":0},{"$f":"'Step1_Input OD450 data'!Z127","v":0},{"$f":"'Step1_Input OD450 data'!AA127","v":0},{"$f":"'Step1_Input OD450 data'!AB127","v":0},{"$f":"'Step1_Input OD450 data'!AC127","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!R128","v":0},{"$f":"'Step1_Input OD450 data'!S128","v":0},{"$f":"'Step1_Input OD450 data'!T128","v":0},{"$f":"'Step1_Input OD450 data'!U128","v":0},{"$f":"'Step1_Input OD450 data'!V128","v":0},{"$f":"'Step1_Input OD450 data'!W128","v":0},{"$f":"'Step1_Input OD450 data'!X128","v":0},{"$f":"'Step1_Input OD450 data'!Y128","v":0},{"$f":"'Step1_Input OD450 data'!Z128","v":0},{"$f":"'Step1_Input OD450 data'!AA128","v":0},{"$f":"'Step1_Input OD450 data'!AB128","v":0},{"$f":"'Step1_Input OD450 data'!AC128","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!R129","v":0},{"$f":"'Step1_Input OD450 data'!S129","v":0},{"$f":"'Step1_Input OD450 data'!T129","v":0},{"$f":"'Step1_Input OD450 data'!U129","v":0},{"$f":"'Step1_Input OD450 data'!V129","v":0},{"$f":"'Step1_Input OD450 data'!W129","v":0},{"$f":"'Step1_Input OD450 data'!X129","v":0},{"$f":"'Step1_Input OD450 data'!Y129","v":0},{"$f":"'Step1_Input OD450 data'!Z129","v":0},{"$f":"'Step1_Input OD450 data'!AA129","v":0},{"$f":"'Step1_Input OD450 data'!AB129","v":0},{"$f":"'Step1_Input OD450 data'!AC129","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!R130","v":0},{"$f":"'Step1_Input OD450 data'!S130","v":0},{"$f":"'Step1_Input OD450 data'!T130","v":0},{"$f":"'Step1_Input OD450 data'!U130","v":0},{"$f":"'Step1_Input OD450 data'!V130","v":0},{"$f":"'Step1_Input OD450 data'!W130","v":0},{"$f":"'Step1_Input OD450 data'!X130","v":0},{"$f":"'Step1_Input OD450 data'!Y130","v":0},{"$f":"'Step1_Input OD450 data'!Z130","v":0},{"$f":"'Step1_Input OD450 data'!AA130","v":0},{"$f":"'Step1_Input OD450 data'!AB130","v":0},{"$f":"'Step1_Input OD450 data'!AC130","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!R131","v":0},{"$f":"'Step1_Input OD450 data'!S131","v":0},{"$f":"'Step1_Input OD450 data'!T131","v":0},{"$f":"'Step1_Input OD450 data'!U131","v":0},{"$f":"'Step1_Input OD450 data'!V131","v":0},{"$f":"'Step1_Input OD450 data'!W131","v":0},{"$f":"'Step1_Input OD450 data'!X131","v":0},{"$f":"'Step1_Input OD450 data'!Y131","v":0},{"$f":"'Step1_Input OD450 data'!Z131","v":0},{"$f":"'Step1_Input OD450 data'!AA131","v":0},{"$f":"'Step1_Input OD450 data'!AB131","v":0},{"$f":"'Step1_Input OD450 data'!AC131","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,172,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,173,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,174,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,175,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,176,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,177,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,178,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,179,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,180,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 21","index":23,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG25","v":0},{"$f":"'Step1_Input OD450 data'!AH25","v":0},{"$f":"'Step1_Input OD450 data'!AI25","v":0},{"$f":"'Step1_Input OD450 data'!AJ25","v":0},{"$f":"'Step1_Input OD450 data'!AK25","v":0},{"$f":"'Step1_Input OD450 data'!AL25","v":0},{"$f":"'Step1_Input OD450 data'!AM25","v":0},{"$f":"'Step1_Input OD450 data'!AN25","v":0},{"$f":"'Step1_Input OD450 data'!AO25","v":0},{"$f":"'Step1_Input OD450 data'!AP25","v":0},{"$f":"'Step1_Input OD450 data'!AQ25","v":0},{"$f":"'Step1_Input OD450 data'!AR25","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG26","v":0},{"$f":"'Step1_Input OD450 data'!AH26","v":0},{"$f":"'Step1_Input OD450 data'!AI26","v":0},{"$f":"'Step1_Input OD450 data'!AJ26","v":0},{"$f":"'Step1_Input OD450 data'!AK26","v":0},{"$f":"'Step1_Input OD450 data'!AL26","v":0},{"$f":"'Step1_Input OD450 data'!AM26","v":0},{"$f":"'Step1_Input OD450 data'!AN26","v":0},{"$f":"'Step1_Input OD450 data'!AO26","v":0},{"$f":"'Step1_Input OD450 data'!AP26","v":0},{"$f":"'Step1_Input OD450 data'!AQ26","v":0},{"$f":"'Step1_Input OD450 data'!AR26","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG27","v":0},{"$f":"'Step1_Input OD450 data'!AH27","v":0},{"$f":"'Step1_Input OD450 data'!AI27","v":0},{"$f":"'Step1_Input OD450 data'!AJ27","v":0},{"$f":"'Step1_Input OD450 data'!AK27","v":0},{"$f":"'Step1_Input OD450 data'!AL27","v":0},{"$f":"'Step1_Input OD450 data'!AM27","v":0},{"$f":"'Step1_Input OD450 data'!AN27","v":0},{"$f":"'Step1_Input OD450 data'!AO27","v":0},{"$f":"'Step1_Input OD450 data'!AP27","v":0},{"$f":"'Step1_Input OD450 data'!AQ27","v":0},{"$f":"'Step1_Input OD450 data'!AR27","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG28","v":0},{"$f":"'Step1_Input OD450 data'!AH28","v":0},{"$f":"'Step1_Input OD450 data'!AI28","v":0},{"$f":"'Step1_Input OD450 data'!AJ28","v":0},{"$f":"'Step1_Input OD450 data'!AK28","v":0},{"$f":"'Step1_Input OD450 data'!AL28","v":0},{"$f":"'Step1_Input OD450 data'!AM28","v":0},{"$f":"'Step1_Input OD450 data'!AN28","v":0},{"$f":"'Step1_Input OD450 data'!AO28","v":0},{"$f":"'Step1_Input OD450 data'!AP28","v":0},{"$f":"'Step1_Input OD450 data'!AQ28","v":0},{"$f":"'Step1_Input OD450 data'!AR28","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG29","v":0},{"$f":"'Step1_Input OD450 data'!AH29","v":0},{"$f":"'Step1_Input OD450 data'!AI29","v":0},{"$f":"'Step1_Input OD450 data'!AJ29","v":0},{"$f":"'Step1_Input OD450 data'!AK29","v":0},{"$f":"'Step1_Input OD450 data'!AL29","v":0},{"$f":"'Step1_Input OD450 data'!AM29","v":0},{"$f":"'Step1_Input OD450 data'!AN29","v":0},{"$f":"'Step1_Input OD450 data'!AO29","v":0},{"$f":"'Step1_Input OD450 data'!AP29","v":0},{"$f":"'Step1_Input OD450 data'!AQ29","v":0},{"$f":"'Step1_Input OD450 data'!AR29","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG30","v":0},{"$f":"'Step1_Input OD450 data'!AH30","v":0},{"$f":"'Step1_Input OD450 data'!AI30","v":0},{"$f":"'Step1_Input OD450 data'!AJ30","v":0},{"$f":"'Step1_Input OD450 data'!AK30","v":0},{"$f":"'Step1_Input OD450 data'!AL30","v":0},{"$f":"'Step1_Input OD450 data'!AM30","v":0},{"$f":"'Step1_Input OD450 data'!AN30","v":0},{"$f":"'Step1_Input OD450 data'!AO30","v":0},{"$f":"'Step1_Input OD450 data'!AP30","v":0},{"$f":"'Step1_Input OD450 data'!AQ30","v":0},{"$f":"'Step1_Input OD450 data'!AR30","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG31","v":0},{"$f":"'Step1_Input OD450 data'!AH31","v":0},{"$f":"'Step1_Input OD450 data'!AI31","v":0},{"$f":"'Step1_Input OD450 data'!AJ31","v":0},{"$f":"'Step1_Input OD450 data'!AK31","v":0},{"$f":"'Step1_Input OD450 data'!AL31","v":0},{"$f":"'Step1_Input OD450 data'!AM31","v":0},{"$f":"'Step1_Input OD450 data'!AN31","v":0},{"$f":"'Step1_Input OD450 data'!AO31","v":0},{"$f":"'Step1_Input OD450 data'!AP31","v":0},{"$f":"'Step1_Input OD450 data'!AQ31","v":0},{"$f":"'Step1_Input OD450 data'!AR31","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG32","v":0},{"$f":"'Step1_Input OD450 data'!AH32","v":0},{"$f":"'Step1_Input OD450 data'!AI32","v":0},{"$f":"'Step1_Input OD450 data'!AJ32","v":0},{"$f":"'Step1_Input OD450 data'!AK32","v":0},{"$f":"'Step1_Input OD450 data'!AL32","v":0},{"$f":"'Step1_Input OD450 data'!AM32","v":0},{"$f":"'Step1_Input OD450 data'!AN32","v":0},{"$f":"'Step1_Input OD450 data'!AO32","v":0},{"$f":"'Step1_Input OD450 data'!AP32","v":0},{"$f":"'Step1_Input OD450 data'!AQ32","v":0},{"$f":"'Step1_Input OD450 data'!AR32","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,181,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,182,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,183,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,184,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,185,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,186,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,187,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,188,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,189,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 22","index":24,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG36","v":0},{"$f":"'Step1_Input OD450 data'!AH36","v":0},{"$f":"'Step1_Input OD450 data'!AI36","v":0},{"$f":"'Step1_Input OD450 data'!AJ36","v":0},{"$f":"'Step1_Input OD450 data'!AK36","v":0},{"$f":"'Step1_Input OD450 data'!AL36","v":0},{"$f":"'Step1_Input OD450 data'!AM36","v":0},{"$f":"'Step1_Input OD450 data'!AN36","v":0},{"$f":"'Step1_Input OD450 data'!AO36","v":0},{"$f":"'Step1_Input OD450 data'!AP36","v":0},{"$f":"'Step1_Input OD450 data'!AQ36","v":0},{"$f":"'Step1_Input OD450 data'!AR36","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG37","v":0},{"$f":"'Step1_Input OD450 data'!AH37","v":0},{"$f":"'Step1_Input OD450 data'!AI37","v":0},{"$f":"'Step1_Input OD450 data'!AJ37","v":0},{"$f":"'Step1_Input OD450 data'!AK37","v":0},{"$f":"'Step1_Input OD450 data'!AL37","v":0},{"$f":"'Step1_Input OD450 data'!AM37","v":0},{"$f":"'Step1_Input OD450 data'!AN37","v":0},{"$f":"'Step1_Input OD450 data'!AO37","v":0},{"$f":"'Step1_Input OD450 data'!AP37","v":0},{"$f":"'Step1_Input OD450 data'!AQ37","v":0},{"$f":"'Step1_Input OD450 data'!AR37","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG38","v":0},{"$f":"'Step1_Input OD450 data'!AH38","v":0},{"$f":"'Step1_Input OD450 data'!AI38","v":0},{"$f":"'Step1_Input OD450 data'!AJ38","v":0},{"$f":"'Step1_Input OD450 data'!AK38","v":0},{"$f":"'Step1_Input OD450 data'!AL38","v":0},{"$f":"'Step1_Input OD450 data'!AM38","v":0},{"$f":"'Step1_Input OD450 data'!AN38","v":0},{"$f":"'Step1_Input OD450 data'!AO38","v":0},{"$f":"'Step1_Input OD450 data'!AP38","v":0},{"$f":"'Step1_Input OD450 data'!AQ38","v":0},{"$f":"'Step1_Input OD450 data'!AR38","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG39","v":0},{"$f":"'Step1_Input OD450 data'!AH39","v":0},{"$f":"'Step1_Input OD450 data'!AI39","v":0},{"$f":"'Step1_Input OD450 data'!AJ39","v":0},{"$f":"'Step1_Input OD450 data'!AK39","v":0},{"$f":"'Step1_Input OD450 data'!AL39","v":0},{"$f":"'Step1_Input OD450 data'!AM39","v":0},{"$f":"'Step1_Input OD450 data'!AN39","v":0},{"$f":"'Step1_Input OD450 data'!AO39","v":0},{"$f":"'Step1_Input OD450 data'!AP39","v":0},{"$f":"'Step1_Input OD450 data'!AQ39","v":0},{"$f":"'Step1_Input OD450 data'!AR39","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG40","v":0},{"$f":"'Step1_Input OD450 data'!AH40","v":0},{"$f":"'Step1_Input OD450 data'!AI40","v":0},{"$f":"'Step1_Input OD450 data'!AJ40","v":0},{"$f":"'Step1_Input OD450 data'!AK40","v":0},{"$f":"'Step1_Input OD450 data'!AL40","v":0},{"$f":"'Step1_Input OD450 data'!AM40","v":0},{"$f":"'Step1_Input OD450 data'!AN40","v":0},{"$f":"'Step1_Input OD450 data'!AO40","v":0},{"$f":"'Step1_Input OD450 data'!AP40","v":0},{"$f":"'Step1_Input OD450 data'!AQ40","v":0},{"$f":"'Step1_Input OD450 data'!AR40","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG41","v":0},{"$f":"'Step1_Input OD450 data'!AH41","v":0},{"$f":"'Step1_Input OD450 data'!AI41","v":0},{"$f":"'Step1_Input OD450 data'!AJ41","v":0},{"$f":"'Step1_Input OD450 data'!AK41","v":0},{"$f":"'Step1_Input OD450 data'!AL41","v":0},{"$f":"'Step1_Input OD450 data'!AM41","v":0},{"$f":"'Step1_Input OD450 data'!AN41","v":0},{"$f":"'Step1_Input OD450 data'!AO41","v":0},{"$f":"'Step1_Input OD450 data'!AP41","v":0},{"$f":"'Step1_Input OD450 data'!AQ41","v":0},{"$f":"'Step1_Input OD450 data'!AR41","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG42","v":0},{"$f":"'Step1_Input OD450 data'!AH42","v":0},{"$f":"'Step1_Input OD450 data'!AI42","v":0},{"$f":"'Step1_Input OD450 data'!AJ42","v":0},{"$f":"'Step1_Input OD450 data'!AK42","v":0},{"$f":"'Step1_Input OD450 data'!AL42","v":0},{"$f":"'Step1_Input OD450 data'!AM42","v":0},{"$f":"'Step1_Input OD450 data'!AN42","v":0},{"$f":"'Step1_Input OD450 data'!AO42","v":0},{"$f":"'Step1_Input OD450 data'!AP42","v":0},{"$f":"'Step1_Input OD450 data'!AQ42","v":0},{"$f":"'Step1_Input OD450 data'!AR42","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG43","v":0},{"$f":"'Step1_Input OD450 data'!AH43","v":0},{"$f":"'Step1_Input OD450 data'!AI43","v":0},{"$f":"'Step1_Input OD450 data'!AJ43","v":0},{"$f":"'Step1_Input OD450 data'!AK43","v":0},{"$f":"'Step1_Input OD450 data'!AL43","v":0},{"$f":"'Step1_Input OD450 data'!AM43","v":0},{"$f":"'Step1_Input OD450 data'!AN43","v":0},{"$f":"'Step1_Input OD450 data'!AO43","v":0},{"$f":"'Step1_Input OD450 data'!AP43","v":0},{"$f":"'Step1_Input OD450 data'!AQ43","v":0},{"$f":"'Step1_Input OD450 data'!AR43","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,190,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,191,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,192,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,193,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,194,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,195,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,196,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,197,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,198,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 23","index":25,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG47","v":0},{"$f":"'Step1_Input OD450 data'!AH47","v":0},{"$f":"'Step1_Input OD450 data'!AI47","v":0},{"$f":"'Step1_Input OD450 data'!AJ47","v":0},{"$f":"'Step1_Input OD450 data'!AK47","v":0},{"$f":"'Step1_Input OD450 data'!AL47","v":0},{"$f":"'Step1_Input OD450 data'!AM47","v":0},{"$f":"'Step1_Input OD450 data'!AN47","v":0},{"$f":"'Step1_Input OD450 data'!AO47","v":0},{"$f":"'Step1_Input OD450 data'!AP47","v":0},{"$f":"'Step1_Input OD450 data'!AQ47","v":0},{"$f":"'Step1_Input OD450 data'!AR47","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG48","v":0},{"$f":"'Step1_Input OD450 data'!AH48","v":0},{"$f":"'Step1_Input OD450 data'!AI48","v":0},{"$f":"'Step1_Input OD450 data'!AJ48","v":0},{"$f":"'Step1_Input OD450 data'!AK48","v":0},{"$f":"'Step1_Input OD450 data'!AL48","v":0},{"$f":"'Step1_Input OD450 data'!AM48","v":0},{"$f":"'Step1_Input OD450 data'!AN48","v":0},{"$f":"'Step1_Input OD450 data'!AO48","v":0},{"$f":"'Step1_Input OD450 data'!AP48","v":0},{"$f":"'Step1_Input OD450 data'!AQ48","v":0},{"$f":"'Step1_Input OD450 data'!AR48","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG49","v":0},{"$f":"'Step1_Input OD450 data'!AH49","v":0},{"$f":"'Step1_Input OD450 data'!AI49","v":0},{"$f":"'Step1_Input OD450 data'!AJ49","v":0},{"$f":"'Step1_Input OD450 data'!AK49","v":0},{"$f":"'Step1_Input OD450 data'!AL49","v":0},{"$f":"'Step1_Input OD450 data'!AM49","v":0},{"$f":"'Step1_Input OD450 data'!AN49","v":0},{"$f":"'Step1_Input OD450 data'!AO49","v":0},{"$f":"'Step1_Input OD450 data'!AP49","v":0},{"$f":"'Step1_Input OD450 data'!AQ49","v":0},{"$f":"'Step1_Input OD450 data'!AR49","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG50","v":0},{"$f":"'Step1_Input OD450 data'!AH50","v":0},{"$f":"'Step1_Input OD450 data'!AI50","v":0},{"$f":"'Step1_Input OD450 data'!AJ50","v":0},{"$f":"'Step1_Input OD450 data'!AK50","v":0},{"$f":"'Step1_Input OD450 data'!AL50","v":0},{"$f":"'Step1_Input OD450 data'!AM50","v":0},{"$f":"'Step1_Input OD450 data'!AN50","v":0},{"$f":"'Step1_Input OD450 data'!AO50","v":0},{"$f":"'Step1_Input OD450 data'!AP50","v":0},{"$f":"'Step1_Input OD450 data'!AQ50","v":0},{"$f":"'Step1_Input OD450 data'!AR50","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG51","v":0},{"$f":"'Step1_Input OD450 data'!AH51","v":0},{"$f":"'Step1_Input OD450 data'!AI51","v":0},{"$f":"'Step1_Input OD450 data'!AJ51","v":0},{"$f":"'Step1_Input OD450 data'!AK51","v":0},{"$f":"'Step1_Input OD450 data'!AL51","v":0},{"$f":"'Step1_Input OD450 data'!AM51","v":0},{"$f":"'Step1_Input OD450 data'!AN51","v":0},{"$f":"'Step1_Input OD450 data'!AO51","v":0},{"$f":"'Step1_Input OD450 data'!AP51","v":0},{"$f":"'Step1_Input OD450 data'!AQ51","v":0},{"$f":"'Step1_Input OD450 data'!AR51","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG52","v":0},{"$f":"'Step1_Input OD450 data'!AH52","v":0},{"$f":"'Step1_Input OD450 data'!AI52","v":0},{"$f":"'Step1_Input OD450 data'!AJ52","v":0},{"$f":"'Step1_Input OD450 data'!AK52","v":0},{"$f":"'Step1_Input OD450 data'!AL52","v":0},{"$f":"'Step1_Input OD450 data'!AM52","v":0},{"$f":"'Step1_Input OD450 data'!AN52","v":0},{"$f":"'Step1_Input OD450 data'!AO52","v":0},{"$f":"'Step1_Input OD450 data'!AP52","v":0},{"$f":"'Step1_Input OD450 data'!AQ52","v":0},{"$f":"'Step1_Input OD450 data'!AR52","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG53","v":0},{"$f":"'Step1_Input OD450 data'!AH53","v":0},{"$f":"'Step1_Input OD450 data'!AI53","v":0},{"$f":"'Step1_Input OD450 data'!AJ53","v":0},{"$f":"'Step1_Input OD450 data'!AK53","v":0},{"$f":"'Step1_Input OD450 data'!AL53","v":0},{"$f":"'Step1_Input OD450 data'!AM53","v":0},{"$f":"'Step1_Input OD450 data'!AN53","v":0},{"$f":"'Step1_Input OD450 data'!AO53","v":0},{"$f":"'Step1_Input OD450 data'!AP53","v":0},{"$f":"'Step1_Input OD450 data'!AQ53","v":0},{"$f":"'Step1_Input OD450 data'!AR53","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG54","v":0},{"$f":"'Step1_Input OD450 data'!AH54","v":0},{"$f":"'Step1_Input OD450 data'!AI54","v":0},{"$f":"'Step1_Input OD450 data'!AJ54","v":0},{"$f":"'Step1_Input OD450 data'!AK54","v":0},{"$f":"'Step1_Input OD450 data'!AL54","v":0},{"$f":"'Step1_Input OD450 data'!AM54","v":0},{"$f":"'Step1_Input OD450 data'!AN54","v":0},{"$f":"'Step1_Input OD450 data'!AO54","v":0},{"$f":"'Step1_Input OD450 data'!AP54","v":0},{"$f":"'Step1_Input OD450 data'!AQ54","v":0},{"$f":"'Step1_Input OD450 data'!AR54","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,199,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,200,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,201,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,202,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,203,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,204,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,205,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,206,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,207,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 24","index":26,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG58","v":0},{"$f":"'Step1_Input OD450 data'!AH58","v":0},{"$f":"'Step1_Input OD450 data'!AI58","v":0},{"$f":"'Step1_Input OD450 data'!AJ58","v":0},{"$f":"'Step1_Input OD450 data'!AK58","v":0},{"$f":"'Step1_Input OD450 data'!AL58","v":0},{"$f":"'Step1_Input OD450 data'!AM58","v":0},{"$f":"'Step1_Input OD450 data'!AN58","v":0},{"$f":"'Step1_Input OD450 data'!AO58","v":0},{"$f":"'Step1_Input OD450 data'!AP58","v":0},{"$f":"'Step1_Input OD450 data'!AQ58","v":0},{"$f":"'Step1_Input OD450 data'!AR58","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG59","v":0},{"$f":"'Step1_Input OD450 data'!AH59","v":0},{"$f":"'Step1_Input OD450 data'!AI59","v":0},{"$f":"'Step1_Input OD450 data'!AJ59","v":0},{"$f":"'Step1_Input OD450 data'!AK59","v":0},{"$f":"'Step1_Input OD450 data'!AL59","v":0},{"$f":"'Step1_Input OD450 data'!AM59","v":0},{"$f":"'Step1_Input OD450 data'!AN59","v":0},{"$f":"'Step1_Input OD450 data'!AO59","v":0},{"$f":"'Step1_Input OD450 data'!AP59","v":0},{"$f":"'Step1_Input OD450 data'!AQ59","v":0},{"$f":"'Step1_Input OD450 data'!AR59","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG60","v":0},{"$f":"'Step1_Input OD450 data'!AH60","v":0},{"$f":"'Step1_Input OD450 data'!AI60","v":0},{"$f":"'Step1_Input OD450 data'!AJ60","v":0},{"$f":"'Step1_Input OD450 data'!AK60","v":0},{"$f":"'Step1_Input OD450 data'!AL60","v":0},{"$f":"'Step1_Input OD450 data'!AM60","v":0},{"$f":"'Step1_Input OD450 data'!AN60","v":0},{"$f":"'Step1_Input OD450 data'!AO60","v":0},{"$f":"'Step1_Input OD450 data'!AP60","v":0},{"$f":"'Step1_Input OD450 data'!AQ60","v":0},{"$f":"'Step1_Input OD450 data'!AR60","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG61","v":0},{"$f":"'Step1_Input OD450 data'!AH61","v":0},{"$f":"'Step1_Input OD450 data'!AI61","v":0},{"$f":"'Step1_Input OD450 data'!AJ61","v":0},{"$f":"'Step1_Input OD450 data'!AK61","v":0},{"$f":"'Step1_Input OD450 data'!AL61","v":0},{"$f":"'Step1_Input OD450 data'!AM61","v":0},{"$f":"'Step1_Input OD450 data'!AN61","v":0},{"$f":"'Step1_Input OD450 data'!AO61","v":0},{"$f":"'Step1_Input OD450 data'!AP61","v":0},{"$f":"'Step1_Input OD450 data'!AQ61","v":0},{"$f":"'Step1_Input OD450 data'!AR61","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG62","v":0},{"$f":"'Step1_Input OD450 data'!AH62","v":0},{"$f":"'Step1_Input OD450 data'!AI62","v":0},{"$f":"'Step1_Input OD450 data'!AJ62","v":0},{"$f":"'Step1_Input OD450 data'!AK62","v":0},{"$f":"'Step1_Input OD450 data'!AL62","v":0},{"$f":"'Step1_Input OD450 data'!AM62","v":0},{"$f":"'Step1_Input OD450 data'!AN62","v":0},{"$f":"'Step1_Input OD450 data'!AO62","v":0},{"$f":"'Step1_Input OD450 data'!AP62","v":0},{"$f":"'Step1_Input OD450 data'!AQ62","v":0},{"$f":"'Step1_Input OD450 data'!AR62","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG63","v":0},{"$f":"'Step1_Input OD450 data'!AH63","v":0},{"$f":"'Step1_Input OD450 data'!AI63","v":0},{"$f":"'Step1_Input OD450 data'!AJ63","v":0},{"$f":"'Step1_Input OD450 data'!AK63","v":0},{"$f":"'Step1_Input OD450 data'!AL63","v":0},{"$f":"'Step1_Input OD450 data'!AM63","v":0},{"$f":"'Step1_Input OD450 data'!AN63","v":0},{"$f":"'Step1_Input OD450 data'!AO63","v":0},{"$f":"'Step1_Input OD450 data'!AP63","v":0},{"$f":"'Step1_Input OD450 data'!AQ63","v":0},{"$f":"'Step1_Input OD450 data'!AR63","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG64","v":0},{"$f":"'Step1_Input OD450 data'!AH64","v":0},{"$f":"'Step1_Input OD450 data'!AI64","v":0},{"$f":"'Step1_Input OD450 data'!AJ64","v":0},{"$f":"'Step1_Input OD450 data'!AK64","v":0},{"$f":"'Step1_Input OD450 data'!AL64","v":0},{"$f":"'Step1_Input OD450 data'!AM64","v":0},{"$f":"'Step1_Input OD450 data'!AN64","v":0},{"$f":"'Step1_Input OD450 data'!AO64","v":0},{"$f":"'Step1_Input OD450 data'!AP64","v":0},{"$f":"'Step1_Input OD450 data'!AQ64","v":0},{"$f":"'Step1_Input OD450 data'!AR64","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG65","v":0},{"$f":"'Step1_Input OD450 data'!AH65","v":0},{"$f":"'Step1_Input OD450 data'!AI65","v":0},{"$f":"'Step1_Input OD450 data'!AJ65","v":0},{"$f":"'Step1_Input OD450 data'!AK65","v":0},{"$f":"'Step1_Input OD450 data'!AL65","v":0},{"$f":"'Step1_Input OD450 data'!AM65","v":0},{"$f":"'Step1_Input OD450 data'!AN65","v":0},{"$f":"'Step1_Input OD450 data'!AO65","v":0},{"$f":"'Step1_Input OD450 data'!AP65","v":0},{"$f":"'Step1_Input OD450 data'!AQ65","v":0},{"$f":"'Step1_Input OD450 data'!AR65","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,208,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,209,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,210,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,211,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,212,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,213,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,214,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,215,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,216,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 25","index":27,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG69","v":0},{"$f":"'Step1_Input OD450 data'!AH69","v":0},{"$f":"'Step1_Input OD450 data'!AI69","v":0},{"$f":"'Step1_Input OD450 data'!AJ69","v":0},{"$f":"'Step1_Input OD450 data'!AK69","v":0},{"$f":"'Step1_Input OD450 data'!AL69","v":0},{"$f":"'Step1_Input OD450 data'!AM69","v":0},{"$f":"'Step1_Input OD450 data'!AN69","v":0},{"$f":"'Step1_Input OD450 data'!AO69","v":0},{"$f":"'Step1_Input OD450 data'!AP69","v":0},{"$f":"'Step1_Input OD450 data'!AQ69","v":0},{"$f":"'Step1_Input OD450 data'!AR69","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG70","v":0},{"$f":"'Step1_Input OD450 data'!AH70","v":0},{"$f":"'Step1_Input OD450 data'!AI70","v":0},{"$f":"'Step1_Input OD450 data'!AJ70","v":0},{"$f":"'Step1_Input OD450 data'!AK70","v":0},{"$f":"'Step1_Input OD450 data'!AL70","v":0},{"$f":"'Step1_Input OD450 data'!AM70","v":0},{"$f":"'Step1_Input OD450 data'!AN70","v":0},{"$f":"'Step1_Input OD450 data'!AO70","v":0},{"$f":"'Step1_Input OD450 data'!AP70","v":0},{"$f":"'Step1_Input OD450 data'!AQ70","v":0},{"$f":"'Step1_Input OD450 data'!AR70","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG71","v":0},{"$f":"'Step1_Input OD450 data'!AH71","v":0},{"$f":"'Step1_Input OD450 data'!AI71","v":0},{"$f":"'Step1_Input OD450 data'!AJ71","v":0},{"$f":"'Step1_Input OD450 data'!AK71","v":0},{"$f":"'Step1_Input OD450 data'!AL71","v":0},{"$f":"'Step1_Input OD450 data'!AM71","v":0},{"$f":"'Step1_Input OD450 data'!AN71","v":0},{"$f":"'Step1_Input OD450 data'!AO71","v":0},{"$f":"'Step1_Input OD450 data'!AP71","v":0},{"$f":"'Step1_Input OD450 data'!AQ71","v":0},{"$f":"'Step1_Input OD450 data'!AR71","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG72","v":0},{"$f":"'Step1_Input OD450 data'!AH72","v":0},{"$f":"'Step1_Input OD450 data'!AI72","v":0},{"$f":"'Step1_Input OD450 data'!AJ72","v":0},{"$f":"'Step1_Input OD450 data'!AK72","v":0},{"$f":"'Step1_Input OD450 data'!AL72","v":0},{"$f":"'Step1_Input OD450 data'!AM72","v":0},{"$f":"'Step1_Input OD450 data'!AN72","v":0},{"$f":"'Step1_Input OD450 data'!AO72","v":0},{"$f":"'Step1_Input OD450 data'!AP72","v":0},{"$f":"'Step1_Input OD450 data'!AQ72","v":0},{"$f":"'Step1_Input OD450 data'!AR72","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG73","v":0},{"$f":"'Step1_Input OD450 data'!AH73","v":0},{"$f":"'Step1_Input OD450 data'!AI73","v":0},{"$f":"'Step1_Input OD450 data'!AJ73","v":0},{"$f":"'Step1_Input OD450 data'!AK73","v":0},{"$f":"'Step1_Input OD450 data'!AL73","v":0},{"$f":"'Step1_Input OD450 data'!AM73","v":0},{"$f":"'Step1_Input OD450 data'!AN73","v":0},{"$f":"'Step1_Input OD450 data'!AO73","v":0},{"$f":"'Step1_Input OD450 data'!AP73","v":0},{"$f":"'Step1_Input OD450 data'!AQ73","v":0},{"$f":"'Step1_Input OD450 data'!AR73","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG74","v":0},{"$f":"'Step1_Input OD450 data'!AH74","v":0},{"$f":"'Step1_Input OD450 data'!AI74","v":0},{"$f":"'Step1_Input OD450 data'!AJ74","v":0},{"$f":"'Step1_Input OD450 data'!AK74","v":0},{"$f":"'Step1_Input OD450 data'!AL74","v":0},{"$f":"'Step1_Input OD450 data'!AM74","v":0},{"$f":"'Step1_Input OD450 data'!AN74","v":0},{"$f":"'Step1_Input OD450 data'!AO74","v":0},{"$f":"'Step1_Input OD450 data'!AP74","v":0},{"$f":"'Step1_Input OD450 data'!AQ74","v":0},{"$f":"'Step1_Input OD450 data'!AR74","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG75","v":0},{"$f":"'Step1_Input OD450 data'!AH75","v":0},{"$f":"'Step1_Input OD450 data'!AI75","v":0},{"$f":"'Step1_Input OD450 data'!AJ75","v":0},{"$f":"'Step1_Input OD450 data'!AK75","v":0},{"$f":"'Step1_Input OD450 data'!AL75","v":0},{"$f":"'Step1_Input OD450 data'!AM75","v":0},{"$f":"'Step1_Input OD450 data'!AN75","v":0},{"$f":"'Step1_Input OD450 data'!AO75","v":0},{"$f":"'Step1_Input OD450 data'!AP75","v":0},{"$f":"'Step1_Input OD450 data'!AQ75","v":0},{"$f":"'Step1_Input OD450 data'!AR75","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG76","v":0},{"$f":"'Step1_Input OD450 data'!AH76","v":0},{"$f":"'Step1_Input OD450 data'!AI76","v":0},{"$f":"'Step1_Input OD450 data'!AJ76","v":0},{"$f":"'Step1_Input OD450 data'!AK76","v":0},{"$f":"'Step1_Input OD450 data'!AL76","v":0},{"$f":"'Step1_Input OD450 data'!AM76","v":0},{"$f":"'Step1_Input OD450 data'!AN76","v":0},{"$f":"'Step1_Input OD450 data'!AO76","v":0},{"$f":"'Step1_Input OD450 data'!AP76","v":0},{"$f":"'Step1_Input OD450 data'!AQ76","v":0},{"$f":"'Step1_Input OD450 data'!AR76","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,217,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,218,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,219,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,220,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,221,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,222,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,223,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,224,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,225,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 26","index":28,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG80","v":0},{"$f":"'Step1_Input OD450 data'!AH80","v":0},{"$f":"'Step1_Input OD450 data'!AI80","v":0},{"$f":"'Step1_Input OD450 data'!AJ80","v":0},{"$f":"'Step1_Input OD450 data'!AK80","v":0},{"$f":"'Step1_Input OD450 data'!AL80","v":0},{"$f":"'Step1_Input OD450 data'!AM80","v":0},{"$f":"'Step1_Input OD450 data'!AN80","v":0},{"$f":"'Step1_Input OD450 data'!AO80","v":0},{"$f":"'Step1_Input OD450 data'!AP80","v":0},{"$f":"'Step1_Input OD450 data'!AQ80","v":0},{"$f":"'Step1_Input OD450 data'!AR80","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG81","v":0},{"$f":"'Step1_Input OD450 data'!AH81","v":0},{"$f":"'Step1_Input OD450 data'!AI81","v":0},{"$f":"'Step1_Input OD450 data'!AJ81","v":0},{"$f":"'Step1_Input OD450 data'!AK81","v":0},{"$f":"'Step1_Input OD450 data'!AL81","v":0},{"$f":"'Step1_Input OD450 data'!AM81","v":0},{"$f":"'Step1_Input OD450 data'!AN81","v":0},{"$f":"'Step1_Input OD450 data'!AO81","v":0},{"$f":"'Step1_Input OD450 data'!AP81","v":0},{"$f":"'Step1_Input OD450 data'!AQ81","v":0},{"$f":"'Step1_Input OD450 data'!AR81","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG82","v":0},{"$f":"'Step1_Input OD450 data'!AH82","v":0},{"$f":"'Step1_Input OD450 data'!AI82","v":0},{"$f":"'Step1_Input OD450 data'!AJ82","v":0},{"$f":"'Step1_Input OD450 data'!AK82","v":0},{"$f":"'Step1_Input OD450 data'!AL82","v":0},{"$f":"'Step1_Input OD450 data'!AM82","v":0},{"$f":"'Step1_Input OD450 data'!AN82","v":0},{"$f":"'Step1_Input OD450 data'!AO82","v":0},{"$f":"'Step1_Input OD450 data'!AP82","v":0},{"$f":"'Step1_Input OD450 data'!AQ82","v":0},{"$f":"'Step1_Input OD450 data'!AR82","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG83","v":0},{"$f":"'Step1_Input OD450 data'!AH83","v":0},{"$f":"'Step1_Input OD450 data'!AI83","v":0},{"$f":"'Step1_Input OD450 data'!AJ83","v":0},{"$f":"'Step1_Input OD450 data'!AK83","v":0},{"$f":"'Step1_Input OD450 data'!AL83","v":0},{"$f":"'Step1_Input OD450 data'!AM83","v":0},{"$f":"'Step1_Input OD450 data'!AN83","v":0},{"$f":"'Step1_Input OD450 data'!AO83","v":0},{"$f":"'Step1_Input OD450 data'!AP83","v":0},{"$f":"'Step1_Input OD450 data'!AQ83","v":0},{"$f":"'Step1_Input OD450 data'!AR83","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG84","v":0},{"$f":"'Step1_Input OD450 data'!AH84","v":0},{"$f":"'Step1_Input OD450 data'!AI84","v":0},{"$f":"'Step1_Input OD450 data'!AJ84","v":0},{"$f":"'Step1_Input OD450 data'!AK84","v":0},{"$f":"'Step1_Input OD450 data'!AL84","v":0},{"$f":"'Step1_Input OD450 data'!AM84","v":0},{"$f":"'Step1_Input OD450 data'!AN84","v":0},{"$f":"'Step1_Input OD450 data'!AO84","v":0},{"$f":"'Step1_Input OD450 data'!AP84","v":0},{"$f":"'Step1_Input OD450 data'!AQ84","v":0},{"$f":"'Step1_Input OD450 data'!AR84","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG85","v":0},{"$f":"'Step1_Input OD450 data'!AH85","v":0},{"$f":"'Step1_Input OD450 data'!AI85","v":0},{"$f":"'Step1_Input OD450 data'!AJ85","v":0},{"$f":"'Step1_Input OD450 data'!AK85","v":0},{"$f":"'Step1_Input OD450 data'!AL85","v":0},{"$f":"'Step1_Input OD450 data'!AM85","v":0},{"$f":"'Step1_Input OD450 data'!AN85","v":0},{"$f":"'Step1_Input OD450 data'!AO85","v":0},{"$f":"'Step1_Input OD450 data'!AP85","v":0},{"$f":"'Step1_Input OD450 data'!AQ85","v":0},{"$f":"'Step1_Input OD450 data'!AR85","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG86","v":0},{"$f":"'Step1_Input OD450 data'!AH86","v":0},{"$f":"'Step1_Input OD450 data'!AI86","v":0},{"$f":"'Step1_Input OD450 data'!AJ86","v":0},{"$f":"'Step1_Input OD450 data'!AK86","v":0},{"$f":"'Step1_Input OD450 data'!AL86","v":0},{"$f":"'Step1_Input OD450 data'!AM86","v":0},{"$f":"'Step1_Input OD450 data'!AN86","v":0},{"$f":"'Step1_Input OD450 data'!AO86","v":0},{"$f":"'Step1_Input OD450 data'!AP86","v":0},{"$f":"'Step1_Input OD450 data'!AQ86","v":0},{"$f":"'Step1_Input OD450 data'!AR86","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG87","v":0},{"$f":"'Step1_Input OD450 data'!AH87","v":0},{"$f":"'Step1_Input OD450 data'!AI87","v":0},{"$f":"'Step1_Input OD450 data'!AJ87","v":0},{"$f":"'Step1_Input OD450 data'!AK87","v":0},{"$f":"'Step1_Input OD450 data'!AL87","v":0},{"$f":"'Step1_Input OD450 data'!AM87","v":0},{"$f":"'Step1_Input OD450 data'!AN87","v":0},{"$f":"'Step1_Input OD450 data'!AO87","v":0},{"$f":"'Step1_Input OD450 data'!AP87","v":0},{"$f":"'Step1_Input OD450 data'!AQ87","v":0},{"$f":"'Step1_Input OD450 data'!AR87","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,226,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,227,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,228,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,229,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,230,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,231,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,232,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,233,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,234,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 27","index":29,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG91","v":0},{"$f":"'Step1_Input OD450 data'!AH91","v":0},{"$f":"'Step1_Input OD450 data'!AI91","v":0},{"$f":"'Step1_Input OD450 data'!AJ91","v":0},{"$f":"'Step1_Input OD450 data'!AK91","v":0},{"$f":"'Step1_Input OD450 data'!AL91","v":0},{"$f":"'Step1_Input OD450 data'!AM91","v":0},{"$f":"'Step1_Input OD450 data'!AN91","v":0},{"$f":"'Step1_Input OD450 data'!AO91","v":0},{"$f":"'Step1_Input OD450 data'!AP91","v":0},{"$f":"'Step1_Input OD450 data'!AQ91","v":0},{"$f":"'Step1_Input OD450 data'!AR91","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG92","v":0},{"$f":"'Step1_Input OD450 data'!AH92","v":0},{"$f":"'Step1_Input OD450 data'!AI92","v":0},{"$f":"'Step1_Input OD450 data'!AJ92","v":0},{"$f":"'Step1_Input OD450 data'!AK92","v":0},{"$f":"'Step1_Input OD450 data'!AL92","v":0},{"$f":"'Step1_Input OD450 data'!AM92","v":0},{"$f":"'Step1_Input OD450 data'!AN92","v":0},{"$f":"'Step1_Input OD450 data'!AO92","v":0},{"$f":"'Step1_Input OD450 data'!AP92","v":0},{"$f":"'Step1_Input OD450 data'!AQ92","v":0},{"$f":"'Step1_Input OD450 data'!AR92","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG93","v":0},{"$f":"'Step1_Input OD450 data'!AH93","v":0},{"$f":"'Step1_Input OD450 data'!AI93","v":0},{"$f":"'Step1_Input OD450 data'!AJ93","v":0},{"$f":"'Step1_Input OD450 data'!AK93","v":0},{"$f":"'Step1_Input OD450 data'!AL93","v":0},{"$f":"'Step1_Input OD450 data'!AM93","v":0},{"$f":"'Step1_Input OD450 data'!AN93","v":0},{"$f":"'Step1_Input OD450 data'!AO93","v":0},{"$f":"'Step1_Input OD450 data'!AP93","v":0},{"$f":"'Step1_Input OD450 data'!AQ93","v":0},{"$f":"'Step1_Input OD450 data'!AR93","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG94","v":0},{"$f":"'Step1_Input OD450 data'!AH94","v":0},{"$f":"'Step1_Input OD450 data'!AI94","v":0},{"$f":"'Step1_Input OD450 data'!AJ94","v":0},{"$f":"'Step1_Input OD450 data'!AK94","v":0},{"$f":"'Step1_Input OD450 data'!AL94","v":0},{"$f":"'Step1_Input OD450 data'!AM94","v":0},{"$f":"'Step1_Input OD450 data'!AN94","v":0},{"$f":"'Step1_Input OD450 data'!AO94","v":0},{"$f":"'Step1_Input OD450 data'!AP94","v":0},{"$f":"'Step1_Input OD450 data'!AQ94","v":0},{"$f":"'Step1_Input OD450 data'!AR94","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG95","v":0},{"$f":"'Step1_Input OD450 data'!AH95","v":0},{"$f":"'Step1_Input OD450 data'!AI95","v":0},{"$f":"'Step1_Input OD450 data'!AJ95","v":0},{"$f":"'Step1_Input OD450 data'!AK95","v":0},{"$f":"'Step1_Input OD450 data'!AL95","v":0},{"$f":"'Step1_Input OD450 data'!AM95","v":0},{"$f":"'Step1_Input OD450 data'!AN95","v":0},{"$f":"'Step1_Input OD450 data'!AO95","v":0},{"$f":"'Step1_Input OD450 data'!AP95","v":0},{"$f":"'Step1_Input OD450 data'!AQ95","v":0},{"$f":"'Step1_Input OD450 data'!AR95","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG96","v":0},{"$f":"'Step1_Input OD450 data'!AH96","v":0},{"$f":"'Step1_Input OD450 data'!AI96","v":0},{"$f":"'Step1_Input OD450 data'!AJ96","v":0},{"$f":"'Step1_Input OD450 data'!AK96","v":0},{"$f":"'Step1_Input OD450 data'!AL96","v":0},{"$f":"'Step1_Input OD450 data'!AM96","v":0},{"$f":"'Step1_Input OD450 data'!AN96","v":0},{"$f":"'Step1_Input OD450 data'!AO96","v":0},{"$f":"'Step1_Input OD450 data'!AP96","v":0},{"$f":"'Step1_Input OD450 data'!AQ96","v":0},{"$f":"'Step1_Input OD450 data'!AR96","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG97","v":0},{"$f":"'Step1_Input OD450 data'!AH97","v":0},{"$f":"'Step1_Input OD450 data'!AI97","v":0},{"$f":"'Step1_Input OD450 data'!AJ97","v":0},{"$f":"'Step1_Input OD450 data'!AK97","v":0},{"$f":"'Step1_Input OD450 data'!AL97","v":0},{"$f":"'Step1_Input OD450 data'!AM97","v":0},{"$f":"'Step1_Input OD450 data'!AN97","v":0},{"$f":"'Step1_Input OD450 data'!AO97","v":0},{"$f":"'Step1_Input OD450 data'!AP97","v":0},{"$f":"'Step1_Input OD450 data'!AQ97","v":0},{"$f":"'Step1_Input OD450 data'!AR97","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG98","v":0},{"$f":"'Step1_Input OD450 data'!AH98","v":0},{"$f":"'Step1_Input OD450 data'!AI98","v":0},{"$f":"'Step1_Input OD450 data'!AJ98","v":0},{"$f":"'Step1_Input OD450 data'!AK98","v":0},{"$f":"'Step1_Input OD450 data'!AL98","v":0},{"$f":"'Step1_Input OD450 data'!AM98","v":0},{"$f":"'Step1_Input OD450 data'!AN98","v":0},{"$f":"'Step1_Input OD450 data'!AO98","v":0},{"$f":"'Step1_Input OD450 data'!AP98","v":0},{"$f":"'Step1_Input OD450 data'!AQ98","v":0},{"$f":"'Step1_Input OD450 data'!AR98","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,235,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,236,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,237,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,238,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,239,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,240,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,241,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,242,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,243,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 28","index":30,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG102","v":0},{"$f":"'Step1_Input OD450 data'!AH102","v":0},{"$f":"'Step1_Input OD450 data'!AI102","v":0},{"$f":"'Step1_Input OD450 data'!AJ102","v":0},{"$f":"'Step1_Input OD450 data'!AK102","v":0},{"$f":"'Step1_Input OD450 data'!AL102","v":0},{"$f":"'Step1_Input OD450 data'!AM102","v":0},{"$f":"'Step1_Input OD450 data'!AN102","v":0},{"$f":"'Step1_Input OD450 data'!AO102","v":0},{"$f":"'Step1_Input OD450 data'!AP102","v":0},{"$f":"'Step1_Input OD450 data'!AQ102","v":0},{"$f":"'Step1_Input OD450 data'!AR102","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG103","v":0},{"$f":"'Step1_Input OD450 data'!AH103","v":0},{"$f":"'Step1_Input OD450 data'!AI103","v":0},{"$f":"'Step1_Input OD450 data'!AJ103","v":0},{"$f":"'Step1_Input OD450 data'!AK103","v":0},{"$f":"'Step1_Input OD450 data'!AL103","v":0},{"$f":"'Step1_Input OD450 data'!AM103","v":0},{"$f":"'Step1_Input OD450 data'!AN103","v":0},{"$f":"'Step1_Input OD450 data'!AO103","v":0},{"$f":"'Step1_Input OD450 data'!AP103","v":0},{"$f":"'Step1_Input OD450 data'!AQ103","v":0},{"$f":"'Step1_Input OD450 data'!AR103","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG104","v":0},{"$f":"'Step1_Input OD450 data'!AH104","v":0},{"$f":"'Step1_Input OD450 data'!AI104","v":0},{"$f":"'Step1_Input OD450 data'!AJ104","v":0},{"$f":"'Step1_Input OD450 data'!AK104","v":0},{"$f":"'Step1_Input OD450 data'!AL104","v":0},{"$f":"'Step1_Input OD450 data'!AM104","v":0},{"$f":"'Step1_Input OD450 data'!AN104","v":0},{"$f":"'Step1_Input OD450 data'!AO104","v":0},{"$f":"'Step1_Input OD450 data'!AP104","v":0},{"$f":"'Step1_Input OD450 data'!AQ104","v":0},{"$f":"'Step1_Input OD450 data'!AR104","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG105","v":0},{"$f":"'Step1_Input OD450 data'!AH105","v":0},{"$f":"'Step1_Input OD450 data'!AI105","v":0},{"$f":"'Step1_Input OD450 data'!AJ105","v":0},{"$f":"'Step1_Input OD450 data'!AK105","v":0},{"$f":"'Step1_Input OD450 data'!AL105","v":0},{"$f":"'Step1_Input OD450 data'!AM105","v":0},{"$f":"'Step1_Input OD450 data'!AN105","v":0},{"$f":"'Step1_Input OD450 data'!AO105","v":0},{"$f":"'Step1_Input OD450 data'!AP105","v":0},{"$f":"'Step1_Input OD450 data'!AQ105","v":0},{"$f":"'Step1_Input OD450 data'!AR105","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG106","v":0},{"$f":"'Step1_Input OD450 data'!AH106","v":0},{"$f":"'Step1_Input OD450 data'!AI106","v":0},{"$f":"'Step1_Input OD450 data'!AJ106","v":0},{"$f":"'Step1_Input OD450 data'!AK106","v":0},{"$f":"'Step1_Input OD450 data'!AL106","v":0},{"$f":"'Step1_Input OD450 data'!AM106","v":0},{"$f":"'Step1_Input OD450 data'!AN106","v":0},{"$f":"'Step1_Input OD450 data'!AO106","v":0},{"$f":"'Step1_Input OD450 data'!AP106","v":0},{"$f":"'Step1_Input OD450 data'!AQ106","v":0},{"$f":"'Step1_Input OD450 data'!AR106","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG107","v":0},{"$f":"'Step1_Input OD450 data'!AH107","v":0},{"$f":"'Step1_Input OD450 data'!AI107","v":0},{"$f":"'Step1_Input OD450 data'!AJ107","v":0},{"$f":"'Step1_Input OD450 data'!AK107","v":0},{"$f":"'Step1_Input OD450 data'!AL107","v":0},{"$f":"'Step1_Input OD450 data'!AM107","v":0},{"$f":"'Step1_Input OD450 data'!AN107","v":0},{"$f":"'Step1_Input OD450 data'!AO107","v":0},{"$f":"'Step1_Input OD450 data'!AP107","v":0},{"$f":"'Step1_Input OD450 data'!AQ107","v":0},{"$f":"'Step1_Input OD450 data'!AR107","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG108","v":0},{"$f":"'Step1_Input OD450 data'!AH108","v":0},{"$f":"'Step1_Input OD450 data'!AI108","v":0},{"$f":"'Step1_Input OD450 data'!AJ108","v":0},{"$f":"'Step1_Input OD450 data'!AK108","v":0},{"$f":"'Step1_Input OD450 data'!AL108","v":0},{"$f":"'Step1_Input OD450 data'!AM108","v":0},{"$f":"'Step1_Input OD450 data'!AN108","v":0},{"$f":"'Step1_Input OD450 data'!AO108","v":0},{"$f":"'Step1_Input OD450 data'!AP108","v":0},{"$f":"'Step1_Input OD450 data'!AQ108","v":0},{"$f":"'Step1_Input OD450 data'!AR108","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG109","v":0},{"$f":"'Step1_Input OD450 data'!AH109","v":0},{"$f":"'Step1_Input OD450 data'!AI109","v":0},{"$f":"'Step1_Input OD450 data'!AJ109","v":0},{"$f":"'Step1_Input OD450 data'!AK109","v":0},{"$f":"'Step1_Input OD450 data'!AL109","v":0},{"$f":"'Step1_Input OD450 data'!AM109","v":0},{"$f":"'Step1_Input OD450 data'!AN109","v":0},{"$f":"'Step1_Input OD450 data'!AO109","v":0},{"$f":"'Step1_Input OD450 data'!AP109","v":0},{"$f":"'Step1_Input OD450 data'!AQ109","v":0},{"$f":"'Step1_Input OD450 data'!AR109","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,244,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,245,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,246,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,247,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,248,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,249,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,250,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,251,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,252,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 29","index":31,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG113","v":0},{"$f":"'Step1_Input OD450 data'!AH113","v":0},{"$f":"'Step1_Input OD450 data'!AI113","v":0},{"$f":"'Step1_Input OD450 data'!AJ113","v":0},{"$f":"'Step1_Input OD450 data'!AK113","v":0},{"$f":"'Step1_Input OD450 data'!AL113","v":0},{"$f":"'Step1_Input OD450 data'!AM113","v":0},{"$f":"'Step1_Input OD450 data'!AN113","v":0},{"$f":"'Step1_Input OD450 data'!AO113","v":0},{"$f":"'Step1_Input OD450 data'!AP113","v":0},{"$f":"'Step1_Input OD450 data'!AQ113","v":0},{"$f":"'Step1_Input OD450 data'!AR113","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG114","v":0},{"$f":"'Step1_Input OD450 data'!AH114","v":0},{"$f":"'Step1_Input OD450 data'!AI114","v":0},{"$f":"'Step1_Input OD450 data'!AJ114","v":0},{"$f":"'Step1_Input OD450 data'!AK114","v":0},{"$f":"'Step1_Input OD450 data'!AL114","v":0},{"$f":"'Step1_Input OD450 data'!AM114","v":0},{"$f":"'Step1_Input OD450 data'!AN114","v":0},{"$f":"'Step1_Input OD450 data'!AO114","v":0},{"$f":"'Step1_Input OD450 data'!AP114","v":0},{"$f":"'Step1_Input OD450 data'!AQ114","v":0},{"$f":"'Step1_Input OD450 data'!AR114","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG115","v":0},{"$f":"'Step1_Input OD450 data'!AH115","v":0},{"$f":"'Step1_Input OD450 data'!AI115","v":0},{"$f":"'Step1_Input OD450 data'!AJ115","v":0},{"$f":"'Step1_Input OD450 data'!AK115","v":0},{"$f":"'Step1_Input OD450 data'!AL115","v":0},{"$f":"'Step1_Input OD450 data'!AM115","v":0},{"$f":"'Step1_Input OD450 data'!AN115","v":0},{"$f":"'Step1_Input OD450 data'!AO115","v":0},{"$f":"'Step1_Input OD450 data'!AP115","v":0},{"$f":"'Step1_Input OD450 data'!AQ115","v":0},{"$f":"'Step1_Input OD450 data'!AR115","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG116","v":0},{"$f":"'Step1_Input OD450 data'!AH116","v":0},{"$f":"'Step1_Input OD450 data'!AI116","v":0},{"$f":"'Step1_Input OD450 data'!AJ116","v":0},{"$f":"'Step1_Input OD450 data'!AK116","v":0},{"$f":"'Step1_Input OD450 data'!AL116","v":0},{"$f":"'Step1_Input OD450 data'!AM116","v":0},{"$f":"'Step1_Input OD450 data'!AN116","v":0},{"$f":"'Step1_Input OD450 data'!AO116","v":0},{"$f":"'Step1_Input OD450 data'!AP116","v":0},{"$f":"'Step1_Input OD450 data'!AQ116","v":0},{"$f":"'Step1_Input OD450 data'!AR116","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG117","v":0},{"$f":"'Step1_Input OD450 data'!AH117","v":0},{"$f":"'Step1_Input OD450 data'!AI117","v":0},{"$f":"'Step1_Input OD450 data'!AJ117","v":0},{"$f":"'Step1_Input OD450 data'!AK117","v":0},{"$f":"'Step1_Input OD450 data'!AL117","v":0},{"$f":"'Step1_Input OD450 data'!AM117","v":0},{"$f":"'Step1_Input OD450 data'!AN117","v":0},{"$f":"'Step1_Input OD450 data'!AO117","v":0},{"$f":"'Step1_Input OD450 data'!AP117","v":0},{"$f":"'Step1_Input OD450 data'!AQ117","v":0},{"$f":"'Step1_Input OD450 data'!AR117","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG118","v":0},{"$f":"'Step1_Input OD450 data'!AH118","v":0},{"$f":"'Step1_Input OD450 data'!AI118","v":0},{"$f":"'Step1_Input OD450 data'!AJ118","v":0},{"$f":"'Step1_Input OD450 data'!AK118","v":0},{"$f":"'Step1_Input OD450 data'!AL118","v":0},{"$f":"'Step1_Input OD450 data'!AM118","v":0},{"$f":"'Step1_Input OD450 data'!AN118","v":0},{"$f":"'Step1_Input OD450 data'!AO118","v":0},{"$f":"'Step1_Input OD450 data'!AP118","v":0},{"$f":"'Step1_Input OD450 data'!AQ118","v":0},{"$f":"'Step1_Input OD450 data'!AR118","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG119","v":0},{"$f":"'Step1_Input OD450 data'!AH119","v":0},{"$f":"'Step1_Input OD450 data'!AI119","v":0},{"$f":"'Step1_Input OD450 data'!AJ119","v":0},{"$f":"'Step1_Input OD450 data'!AK119","v":0},{"$f":"'Step1_Input OD450 data'!AL119","v":0},{"$f":"'Step1_Input OD450 data'!AM119","v":0},{"$f":"'Step1_Input OD450 data'!AN119","v":0},{"$f":"'Step1_Input OD450 data'!AO119","v":0},{"$f":"'Step1_Input OD450 data'!AP119","v":0},{"$f":"'Step1_Input OD450 data'!AQ119","v":0},{"$f":"'Step1_Input OD450 data'!AR119","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG120","v":0},{"$f":"'Step1_Input OD450 data'!AH120","v":0},{"$f":"'Step1_Input OD450 data'!AI120","v":0},{"$f":"'Step1_Input OD450 data'!AJ120","v":0},{"$f":"'Step1_Input OD450 data'!AK120","v":0},{"$f":"'Step1_Input OD450 data'!AL120","v":0},{"$f":"'Step1_Input OD450 data'!AM120","v":0},{"$f":"'Step1_Input OD450 data'!AN120","v":0},{"$f":"'Step1_Input OD450 data'!AO120","v":0},{"$f":"'Step1_Input OD450 data'!AP120","v":0},{"$f":"'Step1_Input OD450 data'!AQ120","v":0},{"$f":"'Step1_Input OD450 data'!AR120","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,253,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,254,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,255,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,256,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,257,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,258,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,259,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,260,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,261,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 30","index":32,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AG124","v":0},{"$f":"'Step1_Input OD450 data'!AH124","v":0},{"$f":"'Step1_Input OD450 data'!AI124","v":0},{"$f":"'Step1_Input OD450 data'!AJ124","v":0},{"$f":"'Step1_Input OD450 data'!AK124","v":0},{"$f":"'Step1_Input OD450 data'!AL124","v":0},{"$f":"'Step1_Input OD450 data'!AM124","v":0},{"$f":"'Step1_Input OD450 data'!AN124","v":0},{"$f":"'Step1_Input OD450 data'!AO124","v":0},{"$f":"'Step1_Input OD450 data'!AP124","v":0},{"$f":"'Step1_Input OD450 data'!AQ124","v":0},{"$f":"'Step1_Input OD450 data'!AR124","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AG125","v":0},{"$f":"'Step1_Input OD450 data'!AH125","v":0},{"$f":"'Step1_Input OD450 data'!AI125","v":0},{"$f":"'Step1_Input OD450 data'!AJ125","v":0},{"$f":"'Step1_Input OD450 data'!AK125","v":0},{"$f":"'Step1_Input OD450 data'!AL125","v":0},{"$f":"'Step1_Input OD450 data'!AM125","v":0},{"$f":"'Step1_Input OD450 data'!AN125","v":0},{"$f":"'Step1_Input OD450 data'!AO125","v":0},{"$f":"'Step1_Input OD450 data'!AP125","v":0},{"$f":"'Step1_Input OD450 data'!AQ125","v":0},{"$f":"'Step1_Input OD450 data'!AR125","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AG126","v":0},{"$f":"'Step1_Input OD450 data'!AH126","v":0},{"$f":"'Step1_Input OD450 data'!AI126","v":0},{"$f":"'Step1_Input OD450 data'!AJ126","v":0},{"$f":"'Step1_Input OD450 data'!AK126","v":0},{"$f":"'Step1_Input OD450 data'!AL126","v":0},{"$f":"'Step1_Input OD450 data'!AM126","v":0},{"$f":"'Step1_Input OD450 data'!AN126","v":0},{"$f":"'Step1_Input OD450 data'!AO126","v":0},{"$f":"'Step1_Input OD450 data'!AP126","v":0},{"$f":"'Step1_Input OD450 data'!AQ126","v":0},{"$f":"'Step1_Input OD450 data'!AR126","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AG127","v":0},{"$f":"'Step1_Input OD450 data'!AH127","v":0},{"$f":"'Step1_Input OD450 data'!AI127","v":0},{"$f":"'Step1_Input OD450 data'!AJ127","v":0},{"$f":"'Step1_Input OD450 data'!AK127","v":0},{"$f":"'Step1_Input OD450 data'!AL127","v":0},{"$f":"'Step1_Input OD450 data'!AM127","v":0},{"$f":"'Step1_Input OD450 data'!AN127","v":0},{"$f":"'Step1_Input OD450 data'!AO127","v":0},{"$f":"'Step1_Input OD450 data'!AP127","v":0},{"$f":"'Step1_Input OD450 data'!AQ127","v":0},{"$f":"'Step1_Input OD450 data'!AR127","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AG128","v":0},{"$f":"'Step1_Input OD450 data'!AH128","v":0},{"$f":"'Step1_Input OD450 data'!AI128","v":0},{"$f":"'Step1_Input OD450 data'!AJ128","v":0},{"$f":"'Step1_Input OD450 data'!AK128","v":0},{"$f":"'Step1_Input OD450 data'!AL128","v":0},{"$f":"'Step1_Input OD450 data'!AM128","v":0},{"$f":"'Step1_Input OD450 data'!AN128","v":0},{"$f":"'Step1_Input OD450 data'!AO128","v":0},{"$f":"'Step1_Input OD450 data'!AP128","v":0},{"$f":"'Step1_Input OD450 data'!AQ128","v":0},{"$f":"'Step1_Input OD450 data'!AR128","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AG129","v":0},{"$f":"'Step1_Input OD450 data'!AH129","v":0},{"$f":"'Step1_Input OD450 data'!AI129","v":0},{"$f":"'Step1_Input OD450 data'!AJ129","v":0},{"$f":"'Step1_Input OD450 data'!AK129","v":0},{"$f":"'Step1_Input OD450 data'!AL129","v":0},{"$f":"'Step1_Input OD450 data'!AM129","v":0},{"$f":"'Step1_Input OD450 data'!AN129","v":0},{"$f":"'Step1_Input OD450 data'!AO129","v":0},{"$f":"'Step1_Input OD450 data'!AP129","v":0},{"$f":"'Step1_Input OD450 data'!AQ129","v":0},{"$f":"'Step1_Input OD450 data'!AR129","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AG130","v":0},{"$f":"'Step1_Input OD450 data'!AH130","v":0},{"$f":"'Step1_Input OD450 data'!AI130","v":0},{"$f":"'Step1_Input OD450 data'!AJ130","v":0},{"$f":"'Step1_Input OD450 data'!AK130","v":0},{"$f":"'Step1_Input OD450 data'!AL130","v":0},{"$f":"'Step1_Input OD450 data'!AM130","v":0},{"$f":"'Step1_Input OD450 data'!AN130","v":0},{"$f":"'Step1_Input OD450 data'!AO130","v":0},{"$f":"'Step1_Input OD450 data'!AP130","v":0},{"$f":"'Step1_Input OD450 data'!AQ130","v":0},{"$f":"'Step1_Input OD450 data'!AR130","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AG131","v":0},{"$f":"'Step1_Input OD450 data'!AH131","v":0},{"$f":"'Step1_Input OD450 data'!AI131","v":0},{"$f":"'Step1_Input OD450 data'!AJ131","v":0},{"$f":"'Step1_Input OD450 data'!AK131","v":0},{"$f":"'Step1_Input OD450 data'!AL131","v":0},{"$f":"'Step1_Input OD450 data'!AM131","v":0},{"$f":"'Step1_Input OD450 data'!AN131","v":0},{"$f":"'Step1_Input OD450 data'!AO131","v":0},{"$f":"'Step1_Input OD450 data'!AP131","v":0},{"$f":"'Step1_Input OD450 data'!AQ131","v":0},{"$f":"'Step1_Input OD450 data'!AR131","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,262,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,263,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,264,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,265,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,266,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,267,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,268,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,269,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,270,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 31","index":33,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AV25","v":0},{"$f":"'Step1_Input OD450 data'!AW25","v":0},{"$f":"'Step1_Input OD450 data'!AX25","v":0},{"$f":"'Step1_Input OD450 data'!AY25","v":0},{"$f":"'Step1_Input OD450 data'!AZ25","v":0},{"$f":"'Step1_Input OD450 data'!BA25","v":0},{"$f":"'Step1_Input OD450 data'!BB25","v":0},{"$f":"'Step1_Input OD450 data'!BC25","v":0},{"$f":"'Step1_Input OD450 data'!BD25","v":0},{"$f":"'Step1_Input OD450 data'!BE25","v":0},{"$f":"'Step1_Input OD450 data'!BF25","v":0},{"$f":"'Step1_Input OD450 data'!BG25","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AV26","v":0},{"$f":"'Step1_Input OD450 data'!AW26","v":0},{"$f":"'Step1_Input OD450 data'!AX26","v":0},{"$f":"'Step1_Input OD450 data'!AY26","v":0},{"$f":"'Step1_Input OD450 data'!AZ26","v":0},{"$f":"'Step1_Input OD450 data'!BA26","v":0},{"$f":"'Step1_Input OD450 data'!BB26","v":0},{"$f":"'Step1_Input OD450 data'!BC26","v":0},{"$f":"'Step1_Input OD450 data'!BD26","v":0},{"$f":"'Step1_Input OD450 data'!BE26","v":0},{"$f":"'Step1_Input OD450 data'!BF26","v":0},{"$f":"'Step1_Input OD450 data'!BG26","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AV27","v":0},{"$f":"'Step1_Input OD450 data'!AW27","v":0},{"$f":"'Step1_Input OD450 data'!AX27","v":0},{"$f":"'Step1_Input OD450 data'!AY27","v":0},{"$f":"'Step1_Input OD450 data'!AZ27","v":0},{"$f":"'Step1_Input OD450 data'!BA27","v":0},{"$f":"'Step1_Input OD450 data'!BB27","v":0},{"$f":"'Step1_Input OD450 data'!BC27","v":0},{"$f":"'Step1_Input OD450 data'!BD27","v":0},{"$f":"'Step1_Input OD450 data'!BE27","v":0},{"$f":"'Step1_Input OD450 data'!BF27","v":0},{"$f":"'Step1_Input OD450 data'!BG27","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AV28","v":0},{"$f":"'Step1_Input OD450 data'!AW28","v":0},{"$f":"'Step1_Input OD450 data'!AX28","v":0},{"$f":"'Step1_Input OD450 data'!AY28","v":0},{"$f":"'Step1_Input OD450 data'!AZ28","v":0},{"$f":"'Step1_Input OD450 data'!BA28","v":0},{"$f":"'Step1_Input OD450 data'!BB28","v":0},{"$f":"'Step1_Input OD450 data'!BC28","v":0},{"$f":"'Step1_Input OD450 data'!BD28","v":0},{"$f":"'Step1_Input OD450 data'!BE28","v":0},{"$f":"'Step1_Input OD450 data'!BF28","v":0},{"$f":"'Step1_Input OD450 data'!BG28","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AV29","v":0},{"$f":"'Step1_Input OD450 data'!AW29","v":0},{"$f":"'Step1_Input OD450 data'!AX29","v":0},{"$f":"'Step1_Input OD450 data'!AY29","v":0},{"$f":"'Step1_Input OD450 data'!AZ29","v":0},{"$f":"'Step1_Input OD450 data'!BA29","v":0},{"$f":"'Step1_Input OD450 data'!BB29","v":0},{"$f":"'Step1_Input OD450 data'!BC29","v":0},{"$f":"'Step1_Input OD450 data'!BD29","v":0},{"$f":"'Step1_Input OD450 data'!BE29","v":0},{"$f":"'Step1_Input OD450 data'!BF29","v":0},{"$f":"'Step1_Input OD450 data'!BG29","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AV30","v":0},{"$f":"'Step1_Input OD450 data'!AW30","v":0},{"$f":"'Step1_Input OD450 data'!AX30","v":0},{"$f":"'Step1_Input OD450 data'!AY30","v":0},{"$f":"'Step1_Input OD450 data'!AZ30","v":0},{"$f":"'Step1_Input OD450 data'!BA30","v":0},{"$f":"'Step1_Input OD450 data'!BB30","v":0},{"$f":"'Step1_Input OD450 data'!BC30","v":0},{"$f":"'Step1_Input OD450 data'!BD30","v":0},{"$f":"'Step1_Input OD450 data'!BE30","v":0},{"$f":"'Step1_Input OD450 data'!BF30","v":0},{"$f":"'Step1_Input OD450 data'!BG30","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AV31","v":0},{"$f":"'Step1_Input OD450 data'!AW31","v":0},{"$f":"'Step1_Input OD450 data'!AX31","v":0},{"$f":"'Step1_Input OD450 data'!AY31","v":0},{"$f":"'Step1_Input OD450 data'!AZ31","v":0},{"$f":"'Step1_Input OD450 data'!BA31","v":0},{"$f":"'Step1_Input OD450 data'!BB31","v":0},{"$f":"'Step1_Input OD450 data'!BC31","v":0},{"$f":"'Step1_Input OD450 data'!BD31","v":0},{"$f":"'Step1_Input OD450 data'!BE31","v":0},{"$f":"'Step1_Input OD450 data'!BF31","v":0},{"$f":"'Step1_Input OD450 data'!BG31","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AV32","v":0},{"$f":"'Step1_Input OD450 data'!AW32","v":0},{"$f":"'Step1_Input OD450 data'!AX32","v":0},{"$f":"'Step1_Input OD450 data'!AY32","v":0},{"$f":"'Step1_Input OD450 data'!AZ32","v":0},{"$f":"'Step1_Input OD450 data'!BA32","v":0},{"$f":"'Step1_Input OD450 data'!BB32","v":0},{"$f":"'Step1_Input OD450 data'!BC32","v":0},{"$f":"'Step1_Input OD450 data'!BD32","v":0},{"$f":"'Step1_Input OD450 data'!BE32","v":0},{"$f":"'Step1_Input OD450 data'!BF32","v":0},{"$f":"'Step1_Input OD450 data'!BG32","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,271,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,272,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,273,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,274,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,275,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,276,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,277,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,278,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,279,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 32","index":34,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":false,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step1_Input OD450 data'!AV36","v":0},{"$f":"'Step1_Input OD450 data'!AW36","v":0},{"$f":"'Step1_Input OD450 data'!AX36","v":0},{"$f":"'Step1_Input OD450 data'!AY36","v":0},{"$f":"'Step1_Input OD450 data'!AZ36","v":0},{"$f":"'Step1_Input OD450 data'!BA36","v":0},{"$f":"'Step1_Input OD450 data'!BB36","v":0},{"$f":"'Step1_Input OD450 data'!BC36","v":0},{"$f":"'Step1_Input OD450 data'!BD36","v":0},{"$f":"'Step1_Input OD450 data'!BE36","v":0},{"$f":"'Step1_Input OD450 data'!BF36","v":0},{"$f":"'Step1_Input OD450 data'!BG36","v":0},450]
[null,"B",{"$f":"'Step1_Input OD450 data'!AV37","v":0},{"$f":"'Step1_Input OD450 data'!AW37","v":0},{"$f":"'Step1_Input OD450 data'!AX37","v":0},{"$f":"'Step1_Input OD450 data'!AY37","v":0},{"$f":"'Step1_Input OD450 data'!AZ37","v":0},{"$f":"'Step1_Input OD450 data'!BA37","v":0},{"$f":"'Step1_Input OD450 data'!BB37","v":0},{"$f":"'Step1_Input OD450 data'!BC37","v":0},{"$f":"'Step1_Input OD450 data'!BD37","v":0},{"$f":"'Step1_Input OD450 data'!BE37","v":0},{"$f":"'Step1_Input OD450 data'!BF37","v":0},{"$f":"'Step1_Input OD450 data'!BG37","v":0},450]
[null,"C",{"$f":"'Step1_Input OD450 data'!AV38","v":0},{"$f":"'Step1_Input OD450 data'!AW38","v":0},{"$f":"'Step1_Input OD450 data'!AX38","v":0},{"$f":"'Step1_Input OD450 data'!AY38","v":0},{"$f":"'Step1_Input OD450 data'!AZ38","v":0},{"$f":"'Step1_Input OD450 data'!BA38","v":0},{"$f":"'Step1_Input OD450 data'!BB38","v":0},{"$f":"'Step1_Input OD450 data'!BC38","v":0},{"$f":"'Step1_Input OD450 data'!BD38","v":0},{"$f":"'Step1_Input OD450 data'!BE38","v":0},{"$f":"'Step1_Input OD450 data'!BF38","v":0},{"$f":"'Step1_Input OD450 data'!BG38","v":0},450]
[null,"D",{"$f":"'Step1_Input OD450 data'!AV39","v":0},{"$f":"'Step1_Input OD450 data'!AW39","v":0},{"$f":"'Step1_Input OD450 data'!AX39","v":0},{"$f":"'Step1_Input OD450 data'!AY39","v":0},{"$f":"'Step1_Input OD450 data'!AZ39","v":0},{"$f":"'Step1_Input OD450 data'!BA39","v":0},{"$f":"'Step1_Input OD450 data'!BB39","v":0},{"$f":"'Step1_Input OD450 data'!BC39","v":0},{"$f":"'Step1_Input OD450 data'!BD39","v":0},{"$f":"'Step1_Input OD450 data'!BE39","v":0},{"$f":"'Step1_Input OD450 data'!BF39","v":0},{"$f":"'Step1_Input OD450 data'!BG39","v":0},450]
[null,"E",{"$f":"'Step1_Input OD450 data'!AV40","v":0},{"$f":"'Step1_Input OD450 data'!AW40","v":0},{"$f":"'Step1_Input OD450 data'!AX40","v":0},{"$f":"'Step1_Input OD450 data'!AY40","v":0},{"$f":"'Step1_Input OD450 data'!AZ40","v":0},{"$f":"'Step1_Input OD450 data'!BA40","v":0},{"$f":"'Step1_Input OD450 data'!BB40","v":0},{"$f":"'Step1_Input OD450 data'!BC40","v":0},{"$f":"'Step1_Input OD450 data'!BD40","v":0},{"$f":"'Step1_Input OD450 data'!BE40","v":0},{"$f":"'Step1_Input OD450 data'!BF40","v":0},{"$f":"'Step1_Input OD450 data'!BG40","v":0},450]
[null,"F",{"$f":"'Step1_Input OD450 data'!AV41","v":0},{"$f":"'Step1_Input OD450 data'!AW41","v":0},{"$f":"'Step1_Input OD450 data'!AX41","v":0},{"$f":"'Step1_Input OD450 data'!AY41","v":0},{"$f":"'Step1_Input OD450 data'!AZ41","v":0},{"$f":"'Step1_Input OD450 data'!BA41","v":0},{"$f":"'Step1_Input OD450 data'!BB41","v":0},{"$f":"'Step1_Input OD450 data'!BC41","v":0},{"$f":"'Step1_Input OD450 data'!BD41","v":0},{"$f":"'Step1_Input OD450 data'!BE41","v":0},{"$f":"'Step1_Input OD450 data'!BF41","v":0},{"$f":"'Step1_Input OD450 data'!BG41","v":0},450]
[null,"G",{"$f":"'Step1_Input OD450 data'!AV42","v":0},{"$f":"'Step1_Input OD450 data'!AW42","v":0},{"$f":"'Step1_Input OD450 data'!AX42","v":0},{"$f":"'Step1_Input OD450 data'!AY42","v":0},{"$f":"'Step1_Input OD450 data'!AZ42","v":0},{"$f":"'Step1_Input OD450 data'!BA42","v":0},{"$f":"'Step1_Input OD450 data'!BB42","v":0},{"$f":"'Step1_Input OD450 data'!BC42","v":0},{"$f":"'Step1_Input OD450 data'!BD42","v":0},{"$f":"'Step1_Input OD450 data'!BE42","v":0},{"$f":"'Step1_Input OD450 data'!BF42","v":0},{"$f":"'Step1_Input OD450 data'!BG42","v":0},450]
[null,"H",{"$f":"'Step1_Input OD450 data'!AV43","v":0},{"$f":"'Step1_Input OD450 data'!AW43","v":0},{"$f":"'Step1_Input OD450 data'!AX43","v":0},{"$f":"'Step1_Input OD450 data'!AY43","v":0},{"$f":"'Step1_Input OD450 data'!AZ43","v":0},{"$f":"'Step1_Input OD450 data'!BA43","v":0},{"$f":"'Step1_Input OD450 data'!BB43","v":0},{"$f":"'Step1_Input OD450 data'!BC43","v":0},{"$f":"'Step1_Input OD450 data'!BD43","v":0},{"$f":"'Step1_Input OD450 data'!BE43","v":0},{"$f":"'Step1_Input OD450 data'!BF43","v":0},{"$f":"'Step1_Input OD450 data'!BG43","v":0},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"C",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"D",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"E",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"F",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"G",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[null,"H",0,0,0,0,0,0,0,0,0,0,0,0,"Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":-1},{"$f":"I140","v":-1},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":-1},{"$f":"I142","v":-1},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":-1},{"$f":"I144","v":-1},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":-1},{"$f":"I146","v":-1},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":-1},{"$f":"I148","v":-1},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":-1},{"$f":"I150","v":-1},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":-1},{"$f":"I152","v":-1},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":-1},{"$f":"I154","v":-1},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C110:D110)","v":0},{"$f":"STDEV(C110:D110)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":0},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":-1},{"$f":"F123^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C123/2.5","v":40},0,1,{"$f":"D124-E124","v":-1},{"$f":"F124^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,{"$f":"C124/2.5","v":16},0,1,-1,1,null,"Lab software"]
[null,null,6.4,0,1,-1,{"$f":"F126^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G123:G130)","v":8}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":0},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":-1}]
[null,null,null,null,"A3",100,{"$f":"E34","v":0},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":0},-1]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":0},0,-1]
[null,null,null,null,"B3",50,{"$f":"E35","v":0},0,-1]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":0},0,-1]
[null,null,null,null,"C3",25,{"$f":"E36","v":0},0,-1]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":0},0,-1]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":0},0,-1]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":0},0,-1]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":0},0,-1]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":0},0,-1]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":0},0,-1]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":0},0,-1]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":0},0,-1]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":0},0,-1]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":0},0,-1]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":0}]
[null,null,null,null,"H1",null,{"$f":"C41","v":0}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":0},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},0,{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,0,0,"",""]
[null,null,null,null,"D1",8,0,0,"",""]
[null,null,null,null,"E1",16,0,0,"",""]
[null,null,null,null,"F1",32,0,0,"",""]
[null,null,280,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":0},0,"",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},0,0,"",""]
[null,null,null,null,"C4",4,0,0,"",""]
[null,null,null,null,"D4",8,0,0,"",""]
[null,null,null,null,"E4",16,0,0,"",""]
[null,null,null,null,"F4",32,0,0,"",""]
[null,null,null,null,"G4",64,0,0,"",""]
[null,null,null,null,"H4",128,0,0,"",""]
[null,null,281,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":0},0,"",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},0,0,"",""]
[null,null,null,null,"C5",4,0,0,"",""]
[null,null,null,null,"D5",8,0,0,"",""]
[null,null,null,null,"E5",16,0,0,"",""]
[null,null,null,null,"F5",32,0,0,"",""]
[null,null,null,null,"G5",64,0,0,"",""]
[null,null,null,null,"H5",128,0,0,"",""]
[null,null,282,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":0},0,"",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},0,0,"",""]
[null,null,null,null,"C6",4,0,0,"",""]
[null,null,null,null,"D6",8,0,0,"",""]
[null,null,null,null,"E6",16,0,0,"",""]
[null,null,null,null,"F6",32,0,0,"",""]
[null,null,null,null,"G6",64,0,0,"",""]
[null,null,null,null,"H6",128,0,0,"",""]
[null,null,283,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":0},0,"",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},0,0,"",""]
[null,null,null,null,"C7",4,0,0,"",""]
[null,null,null,null,"D7",8,0,0,"",""]
[null,null,null,null,"E7",16,0,0,"",""]
[null,null,null,null,"F7",32,0,0,"",""]
[null,null,null,null,"G7",64,0,0,"",""]
[null,null,null,null,"H7",128,0,0,"",""]
[null,null,284,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":0},0,"",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},0,0,"",""]
[null,null,null,null,"C8",4,0,0,"",""]
[null,null,null,null,"D8",8,0,0,"",""]
[null,null,null,null,"E8",16,0,0,"",""]
[null,null,null,null,"F8",32,0,0,"",""]
[null,null,null,null,"G8",64,0,0,"",""]
[null,null,null,null,"H8",128,0,0,"",""]
[null,null,285,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":0},0,"",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},0,0,"",""]
[null,null,null,null,"C9",4,0,0,"",""]
[null,null,null,null,"D9",8,0,0,"",""]
[null,null,null,null,"E9",16,0,0,"",""]
[null,null,null,null,"F9",32,0,0,"",""]
[null,null,null,null,"G9",64,0,0,"",""]
[null,null,null,null,"H9",128,0,0,"",""]
[null,null,286,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":0},0,"",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},0,0,"",""]
[null,null,null,null,"C10",4,0,0,"",""]
[null,null,null,null,"D10",8,0,0,"",""]
[null,null,null,null,"E10",16,0,0,"",""]
[null,null,null,null,"F10",32,0,0,"",""]
[null,null,null,null,"G10",64,0,0,"",""]
[null,null,null,null,"H10",128,0,0,"",""]
[null,null,287,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":0},0,"",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},0,0,"",""]
[null,null,null,null,"C11",4,0,0,"",""]
[null,null,null,null,"D11",8,0,{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":0},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,0,0,"",""]
[null,null,null,null,"F11",32,0,0,"",""]
[null,null,null,null,"G11",64,0,0,"",""]
[null,null,null,null,"H11",128,0,0,"",""]
[null,null,288,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":0},0,"",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},0,0,"",""]
[null,null,null,null,"C12",4,0,0,"",""]
[null,null,null,null,"D12",8,0,0,"",""]
[null,null,null,null,"E12",16,0,0,"",""]
[null,null,null,null,"F12",32,0,0,"",""]
[null,null,null,null,"G12",64,0,0,"",""]
[null,null,null,null,"H12",128,0,0,{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step 3_IgG titers","index":35,"n_rows":460,"n_cols":43,"rows_kept":460,"cut":false,"hidden":false,"merged":[[1,1,2,8],[27,32,27,33],[13,3,13,4],[13,18,13,19],[27,3,27,4],[27,18,27,19],[13,32,13,33],[3,1,8,8],[69,3,69,4],[69,18,69,19],[69,32,69,33],[41,3,41,4],[41,18,41,19],[41,32,41,33],[55,3,55,4],[55,18,55,19],[55,32,55,33],[111,3,111,4],[111,18,111,19],[111,32,111,33],[125,3,125,4],[125,18,125,19],[125,32,125,33],[83,3,83,4],[83,18,83,19],[83,32,83,33],[97,3,97,4],[97,18,97,19],[97,32,97,33],[167,3,167,4],[167,18,167,19],[167,32,167,33],[181,3,181,4],[181,18,181,19],[181,32,181,33],[139,3,139,4],[139,18,139,19],[139,32,139,33],[153,3,153,4],[153,18,153,19],[153,32,153,33],[223,3,223,4],[223,18,223,19],[223,32,223,33],[237,3,237,4],[237,18,237,19],[237,32,237,33],[195,3,195,4],[195,18,195,19],[195,32,195,33],[209,3,209,4],[209,18,209,19],[209,32,209,33],[279,3,279,4],[279,18,279,19],[279,32,279,33],[293,3,293,4],[293,18,293,19],[293,32,293,33],[251,3,251,4],[251,18,251,19],[251,32,251,33],[265,3,265,4],[265,18,265,19],[265,32,265,33],[335,3,335,4],[335,18,335,19],[335,32,335,33],[349,3,349,4],[349,18,349,19],[349,32,349,33],[307,3,307,4],[307,18,307,19],[307,32,307,33],[321,3,321,4],[321,18,321,19],[321,32,321,33],[391,3,391,4],[391,18,391,19],[391,32,391,33],[405,3,405,4],[405,18,405,19],[405,32,405,33],[363,3,363,4],[363,18,363,19],[363,32,363,33],[377,3,377,4],[377,18,377,19],[377,32,377,33],[447,3,447,4],[447,18,447,19],[447,32,447,33],[419,3,419,4],[419,18,419,19],[419,32,419,33],[433,3,433,4],[433,18,433,19],[433,32,433,33]]}
[]
[null,"Step 3: Define the linear range and determine antibody titer"]
[]
[null,"Determine the linear part of the titration curves by calculating 20% and 80% of the sum of the upper and lower asymptotes. \nPlease note that responses have been limited to fall within the observed range of the full curve for the PC to avoid extrapolation when the data don’t support accurate estimation of the upper asymptote!"]
[]
[]
[]
[]
[]
[]
[]
[null,"Blank OD450 ",null,null,null,null,null,null,null,null,null,null,null,null,null,"Linear range",null,"Conc x dil (EU/mL)",null,null,null,null,null,null,null,null,null,null,null,null,null,"Set linear range values using 'IF' function"]
[]
["Plate 1",null,"NC","PC",null,1,2,3,4,5,6,7,8,9,null,null,null,"NC","PC",null,1,2,3,4,5,6,7,8,9,null,null,"NC","PC",null,1,2,3,4,5,6,7,8,9]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"20 -80 % range",null,1,2,3,4,5,6,7,8,9,10,11,12,null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Step2_Curvefit_plate 1'!C46","v":0},{"$f":"'Step2_Curvefit_plate 1'!D46","v":0},{"$f":"'Step2_Curvefit_plate 1'!E46","v":0},{"$f":"'Step2_Curvefit_plate 1'!F46","v":0},{"$f":"'Step2_Curvefit_plate 1'!G46","v":0},{"$f":"'Step2_Curvefit_plate 1'!H46","v":0},{"$f":"'Step2_Curvefit_plate 1'!I46","v":0},{"$f":"'Step2_Curvefit_plate 1'!J46","v":0},{"$f":"'Step2_Curvefit_plate 1'!K46","v":0},{"$f":"'Step2_Curvefit_plate 1'!L46","v":0},{"$f":"'Step2_Curvefit_plate 1'!M46","v":0},{"$f":"'Step2_Curvefit_plate 1'!N46","v":0},null,"parameter A",{"$f":"'Step2_Curvefit_plate 1'!$J$124","v":1},null,null,null,{"$f":"'Step2_Curvefit_plate 1'!F69","v":""},{"$f":"'Step2_Curvefit_plate 1'!G69","v":""},{"$f":"'Step2_Curvefit_plate 1'!H69","v":""},{"$f":"'Step2_Curvefit_plate 1'!I69","v":""},{"$f":"'Step2_Curvefit_plate 1'!J69","v":""},{"$f":"'Step2_Curvefit_plate 1'!K69","v":""},{"$f":"'Step2_Curvefit_plate 1'!L69","v":""},{"$f":"'Step2_Curvefit_plate 1'!M69","v":""},{"$f":"'Step2_Curvefit_plate 1'!N69","v":""},null,null,{"$f":"IF(C16>$Q$20,IF(C16<$Q$21,IF(C16<$Q$22,IF(C16>$Q$23,R16,\"\"),\"\"),\"\"),\"\")","v":""},null,null,{"$f":"IF(AND(F$16<0,F$17<0,F$18<0,F$19<0,F$20<0,F$21<0,F$22<0,F$23<0),\"\",IF(AND(F16>$Q$20,F16<$Q$21),U16,IF(AND(F$16<$Q$20,F$17<$Q$20,F$18<$Q$20,F$19<$Q$20,F$20<$Q$20,F$21<$Q$20,F$22<$Q$20,F$23<$Q$20),2.5,IF(AND(F$16>$Q$21,F$17>$Q$21,F$18>$Q$21,F$19>$Q$21,F$20>$Q$21,F$21>$Q$21,F$22>$Q$21,F$23>$Q$21),128*$Q$25,\"\"))))","v":2.5},{"$f":"IF(AND(G$16<0,G$17<0,G$18<0,G$19<0,G$20<0,G$21<0,G$22<0,G$23<0),\"\",IF(AND(G16>$Q$20,G16<$Q$21),V16,IF(AND(G$16<$Q$20,G$17<$Q$20,G$18<$Q$20,G$19<$Q$20,G$20<$Q$20,G$21<$Q$20,G$22<$Q$20,G$23<$Q$20),2.5,IF(AND(G$16>$Q$21,G$17>$Q$21,G$18>$Q$21,G$19>$Q$21,G$20>$Q$21,G$21>$Q$21,G$22>$Q$21,G$23>$Q$21),128*$Q$25,\"\"))))","v":2.5},2.5,2.5,2.5,2.5,2.5,2.5,2.5]
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[]
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[]
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[]
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[]
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[]
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[]
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[]
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{"sheet":"Summary IgG titers","index":36,"n_rows":103,"n_cols":20,"rows_kept":103,"cut":false,"hidden":false,"merged":[]}
[null,"Compliation of antibody titers",null,null,null,null,null,null,null,null,null,null,"Compliation of adjusted antibody titers"]
[null,null,null,null,null,null,null,null,null,null,null,null,"Initial Dilution of PC:",null,null,null,null,400]
[null,null,null,null,null,null,null,null,null,null,null,null,"Initial Dilution of test samples:",null,null,null,null,400]
[null,null,null,null,null,null,null,null,null,null,null,null,"Adustment Factor:",null,null,null,null,{"$f":"R3/R2","v":1}]
[]
[null,"RWRS / WHO wP IS4 / In-house reference",null,null,null,null,null,null,null,null,null,null,"RWRS / WHO wP IS4 / In-house reference"]
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[]
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[]
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[]
[]
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