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{"sheet":"Sample Designs","index":0,"n_rows":44,"n_cols":14,"rows_kept":44,"cut":false,"hidden":false,"merged":[[6,4,6,8],[13,2,13,13],[14,2,14,4],[14,5,14,7],[14,8,14,10],[14,11,14,13],[31,2,31,13],[32,2,32,4],[32,5,32,7],[32,8,32,10],[32,11,32,13]]}
[null,"Whole cell ELISA: Titration of directly coupled anti-mouse-IgG-HRP conjugate"]
[]
[null,"Step","Material","Incubation"]
[null,"Capture","Bp18323 (0.25OU/mL)","o/n, 37°C"]
[null,"block","1% BSA","1h, 37°C"]
[null,"antigen /antibody sample","mouse serum (1/400)","1h, 37°C"]
[null,"Conjugate for detection","Goat-anti-mouse-IgG-HRP","1h, 37°C"]
[null,"substrate","TMB","10 min, RT"]
[]
[]
[]
[null,"Whole-cell ELISA: Titration of  directly coupled anti-mouse-IgG-HRP conjugate (Plate 1)"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,null,"Positive mouse sera (PC)"]
[null,null,500,null,null,1000,null,null,2000,null,null,4000]
[null,null,400,800,1600,400,800,1600,400,800,1600,400,800,1600]
[null,null,{"$f":"C16*2.5","v":1000},2000,4000,1000,2000,4000,1000,2000,4000,1000,2000,4000]
[null,null,2500,5000,10000,2500,5000,10000,2500,5000,10000,2500,5000,10000]
[null,null,6250,12500,25000,6250,12500,25000,6250,12500,25000,6250,12500,25000]
[null,null,15625,31250,62500,15625,31250,62500,15625,31250,62500,15625,31250,62500]
[null,null,39062.5,78125,156250,39062.5,78125,156250,39062.5,78125,156250,39062.5,78125,156250]
[null,null,97656.25,195312.5,390625,97656.25,195312.5,390625,97656.25,195312.5,390625,97656.25,195312.5,390625]
[null,null,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5]
[]
[]
[null,null,"1:4000 dilution of anti-m-IgG-HRP is recommended  in current SOP!"]
[]
[]
[]
[null,"Whole-cell ELISA: Titration of  directly coupled anti-mouse-IgG-HRP conjugate (Plate 2)"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,null,"Positive mouse sera (PC)"]
[null,null,8000,null,null,1600,null,null,32000,null,null,64000]
[null,null,400,800,1600,400,800,1600,400,800,1600,400,800,1600]
[null,null,{"$f":"C34*2.5","v":1000},2000,4000,1000,2000,4000,1000,2000,4000,1000,2000,4000]
[null,null,2500,5000,10000,2500,5000,10000,2500,5000,10000,2500,5000,10000]
[null,null,6250,12500,25000,6250,12500,25000,6250,12500,25000,6250,12500,25000]
[null,null,15625,31250,62500,15625,31250,62500,15625,31250,62500,15625,31250,62500]
[null,null,39062.5,78125,156250,39062.5,78125,156250,39062.5,78125,156250,39062.5,78125,156250]
[null,null,97656.25,195312.5,390625,97656.25,195312.5,390625,97656.25,195312.5,390625,97656.25,195312.5,390625]
[null,null,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5,244140.625,488281.25,976562.5]
[]
[]
[null,null,"1:4000 dilution of anti-m-IgG-HRP is recommended  in current SOP!"]

{"sheet":"Input OD450 data","index":1,"n_rows":151,"n_cols":5,"rows_kept":151,"cut":false,"hidden":false,"merged":[[10,9,10,10],[10,11,10,12],[8,3,8,4],[8,5,8,6],[1,1,7,7]]}
[]
[null,"Copy and paste absorbance 450 values of NC, Blank, (Column 1) and duplicate PC series (Columns 2 & 3) for each combination of factors (e.g., conjugate dilution and initial PC dilution).  Enter 0 into BLK cells if NC and blank were omitted from the experiment.  Each input area represents a PC curve for a single combination of factor(s)."]
[]
[]
[]
[]
[]
[]
[]
[null,"Input layout",1,2,3]
[null,"A","NC dil1","PC dil1","PC dil1"]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7"]
[null,"H","BLK","PC dil8","PC dil8"]
[]
[]
[null,"Curve 1"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 2"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 3"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 4"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 5"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 6"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 7"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 8"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 9"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 10"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 11"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]
[]
[null,"Curve 12"]
[null,null,1,2,3]
[null,"A"]
[null,"B"]
[null,"C"]
[null,"D"]
[null,"E"]
[null,"F"]
[null,"G"]
[null,"H"]

{"sheet":"Curve 1","index":2,"n_rows":108,"n_cols":13,"rows_kept":108,"cut":false,"hidden":false,"merged":[[0,0,0,14],[104,8,104,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C23","v":0},{"$f":"'Input OD450 data'!D23","v":0},{"$f":"'Input OD450 data'!E23","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C24","v":0},{"$f":"'Input OD450 data'!D24","v":0},{"$f":"'Input OD450 data'!E24","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C25","v":0},{"$f":"'Input OD450 data'!D25","v":0},{"$f":"'Input OD450 data'!E25","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C26","v":0},{"$f":"'Input OD450 data'!D26","v":0},{"$f":"'Input OD450 data'!E26","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C27","v":0},{"$f":"'Input OD450 data'!D27","v":0},{"$f":"'Input OD450 data'!E27","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C28","v":0},{"$f":"'Input OD450 data'!D28","v":0},{"$f":"'Input OD450 data'!E28","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C29","v":0},{"$f":"'Input OD450 data'!D29","v":0},{"$f":"'Input OD450 data'!E29","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C30","v":0},{"$f":"'Input OD450 data'!D30","v":0},{"$f":"'Input OD450 data'!E30","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

{"sheet":"Curve 2","index":3,"n_rows":108,"n_cols":13,"rows_kept":108,"cut":false,"hidden":false,"merged":[[0,0,0,14],[104,8,104,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C34","v":0},{"$f":"'Input OD450 data'!D34","v":0},{"$f":"'Input OD450 data'!E34","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C35","v":0},{"$f":"'Input OD450 data'!D35","v":0},{"$f":"'Input OD450 data'!E35","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C36","v":0},{"$f":"'Input OD450 data'!D36","v":0},{"$f":"'Input OD450 data'!E36","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C37","v":0},{"$f":"'Input OD450 data'!D37","v":0},{"$f":"'Input OD450 data'!E37","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C38","v":0},{"$f":"'Input OD450 data'!D38","v":0},{"$f":"'Input OD450 data'!E38","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C39","v":0},{"$f":"'Input OD450 data'!D39","v":0},{"$f":"'Input OD450 data'!E39","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C40","v":0},{"$f":"'Input OD450 data'!D40","v":0},{"$f":"'Input OD450 data'!E40","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C41","v":0},{"$f":"'Input OD450 data'!D41","v":0},{"$f":"'Input OD450 data'!E41","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

{"sheet":"Curve 3","index":4,"n_rows":108,"n_cols":13,"rows_kept":108,"cut":false,"hidden":false,"merged":[[0,0,0,14],[104,8,104,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C45","v":0},{"$f":"'Input OD450 data'!D45","v":0},{"$f":"'Input OD450 data'!E45","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C46","v":0},{"$f":"'Input OD450 data'!D46","v":0},{"$f":"'Input OD450 data'!E46","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C47","v":0},{"$f":"'Input OD450 data'!D47","v":0},{"$f":"'Input OD450 data'!E47","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C48","v":0},{"$f":"'Input OD450 data'!D48","v":0},{"$f":"'Input OD450 data'!E48","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C49","v":0},{"$f":"'Input OD450 data'!D49","v":0},{"$f":"'Input OD450 data'!E49","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C50","v":0},{"$f":"'Input OD450 data'!D50","v":0},{"$f":"'Input OD450 data'!E50","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C51","v":0},{"$f":"'Input OD450 data'!D51","v":0},{"$f":"'Input OD450 data'!E51","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C52","v":0},{"$f":"'Input OD450 data'!D52","v":0},{"$f":"'Input OD450 data'!E52","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C56","v":0},{"$f":"'Input OD450 data'!D56","v":0},{"$f":"'Input OD450 data'!E56","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C57","v":0},{"$f":"'Input OD450 data'!D57","v":0},{"$f":"'Input OD450 data'!E57","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C58","v":0},{"$f":"'Input OD450 data'!D58","v":0},{"$f":"'Input OD450 data'!E58","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C59","v":0},{"$f":"'Input OD450 data'!D59","v":0},{"$f":"'Input OD450 data'!E59","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C60","v":0},{"$f":"'Input OD450 data'!D60","v":0},{"$f":"'Input OD450 data'!E60","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C61","v":0},{"$f":"'Input OD450 data'!D61","v":0},{"$f":"'Input OD450 data'!E61","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C62","v":0},{"$f":"'Input OD450 data'!D62","v":0},{"$f":"'Input OD450 data'!E62","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C63","v":0},{"$f":"'Input OD450 data'!D63","v":0},{"$f":"'Input OD450 data'!E63","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C67","v":0},{"$f":"'Input OD450 data'!D67","v":0},{"$f":"'Input OD450 data'!E67","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C68","v":0},{"$f":"'Input OD450 data'!D68","v":0},{"$f":"'Input OD450 data'!E68","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C69","v":0},{"$f":"'Input OD450 data'!D69","v":0},{"$f":"'Input OD450 data'!E69","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C70","v":0},{"$f":"'Input OD450 data'!D70","v":0},{"$f":"'Input OD450 data'!E70","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C71","v":0},{"$f":"'Input OD450 data'!D71","v":0},{"$f":"'Input OD450 data'!E71","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C72","v":0},{"$f":"'Input OD450 data'!D72","v":0},{"$f":"'Input OD450 data'!E72","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C73","v":0},{"$f":"'Input OD450 data'!D73","v":0},{"$f":"'Input OD450 data'!E73","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C74","v":0},{"$f":"'Input OD450 data'!D74","v":0},{"$f":"'Input OD450 data'!E74","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C78","v":0},{"$f":"'Input OD450 data'!D78","v":0},{"$f":"'Input OD450 data'!E78","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C79","v":0},{"$f":"'Input OD450 data'!D79","v":0},{"$f":"'Input OD450 data'!E79","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C80","v":0},{"$f":"'Input OD450 data'!D80","v":0},{"$f":"'Input OD450 data'!E80","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C81","v":0},{"$f":"'Input OD450 data'!D81","v":0},{"$f":"'Input OD450 data'!E81","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C82","v":0},{"$f":"'Input OD450 data'!D82","v":0},{"$f":"'Input OD450 data'!E82","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C83","v":0},{"$f":"'Input OD450 data'!D83","v":0},{"$f":"'Input OD450 data'!E83","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C84","v":0},{"$f":"'Input OD450 data'!D84","v":0},{"$f":"'Input OD450 data'!E84","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C85","v":0},{"$f":"'Input OD450 data'!D85","v":0},{"$f":"'Input OD450 data'!E85","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C89","v":0},{"$f":"'Input OD450 data'!D89","v":0},{"$f":"'Input OD450 data'!E89","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C90","v":0},{"$f":"'Input OD450 data'!D90","v":0},{"$f":"'Input OD450 data'!E90","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C91","v":0},{"$f":"'Input OD450 data'!D91","v":0},{"$f":"'Input OD450 data'!E91","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C92","v":0},{"$f":"'Input OD450 data'!D92","v":0},{"$f":"'Input OD450 data'!E92","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C93","v":0},{"$f":"'Input OD450 data'!D93","v":0},{"$f":"'Input OD450 data'!E93","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C94","v":0},{"$f":"'Input OD450 data'!D94","v":0},{"$f":"'Input OD450 data'!E94","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C95","v":0},{"$f":"'Input OD450 data'!D95","v":0},{"$f":"'Input OD450 data'!E95","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C96","v":0},{"$f":"'Input OD450 data'!D96","v":0},{"$f":"'Input OD450 data'!E96","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

{"sheet":"Curve 8","index":9,"n_rows":108,"n_cols":13,"rows_kept":108,"cut":false,"hidden":false,"merged":[[0,0,0,14],[104,8,104,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C100","v":0},{"$f":"'Input OD450 data'!D100","v":0},{"$f":"'Input OD450 data'!E100","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C101","v":0},{"$f":"'Input OD450 data'!D101","v":0},{"$f":"'Input OD450 data'!E101","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C102","v":0},{"$f":"'Input OD450 data'!D102","v":0},{"$f":"'Input OD450 data'!E102","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C103","v":0},{"$f":"'Input OD450 data'!D103","v":0},{"$f":"'Input OD450 data'!E103","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C104","v":0},{"$f":"'Input OD450 data'!D104","v":0},{"$f":"'Input OD450 data'!E104","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C105","v":0},{"$f":"'Input OD450 data'!D105","v":0},{"$f":"'Input OD450 data'!E105","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C106","v":0},{"$f":"'Input OD450 data'!D106","v":0},{"$f":"'Input OD450 data'!E106","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C107","v":0},{"$f":"'Input OD450 data'!D107","v":0},{"$f":"'Input OD450 data'!E107","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C111","v":0},{"$f":"'Input OD450 data'!D111","v":0},{"$f":"'Input OD450 data'!E111","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C112","v":0},{"$f":"'Input OD450 data'!D112","v":0},{"$f":"'Input OD450 data'!E112","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C113","v":0},{"$f":"'Input OD450 data'!D113","v":0},{"$f":"'Input OD450 data'!E113","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C114","v":0},{"$f":"'Input OD450 data'!D114","v":0},{"$f":"'Input OD450 data'!E114","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C115","v":0},{"$f":"'Input OD450 data'!D115","v":0},{"$f":"'Input OD450 data'!E115","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C116","v":0},{"$f":"'Input OD450 data'!D116","v":0},{"$f":"'Input OD450 data'!E116","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C117","v":0},{"$f":"'Input OD450 data'!D117","v":0},{"$f":"'Input OD450 data'!E117","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C118","v":0},{"$f":"'Input OD450 data'!D118","v":0},{"$f":"'Input OD450 data'!E118","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
[null,"B",2,{"$f":"D23/B$6","v":40},{"$f":"E23/B$6","v":40}]
[null,"C",4,16,16]
[null,"D",8,6.4,6.4]
[null,"E",16,2.56,2.56]
[null,"F",32,1.024,1.024]
[null,"G",null,0.40960000000000002,0.40960000000000002]
[null,"H",null,0.16384000000000001,0.16384000000000001]
[]
["OD450"]
[null,null,1,2,3]
[null,"A",{"$f":"'Input OD450 data'!C122","v":0},{"$f":"'Input OD450 data'!D122","v":0},{"$f":"'Input OD450 data'!E122","v":0}]
[null,"B",{"$f":"'Input OD450 data'!C123","v":0},{"$f":"'Input OD450 data'!D123","v":0},{"$f":"'Input OD450 data'!E123","v":0}]
[null,"C",{"$f":"'Input OD450 data'!C124","v":0},{"$f":"'Input OD450 data'!D124","v":0},{"$f":"'Input OD450 data'!E124","v":0}]
[null,"D",{"$f":"'Input OD450 data'!C125","v":0},{"$f":"'Input OD450 data'!D125","v":0},{"$f":"'Input OD450 data'!E125","v":0}]
[null,"E",{"$f":"'Input OD450 data'!C126","v":0},{"$f":"'Input OD450 data'!D126","v":0},{"$f":"'Input OD450 data'!E126","v":0}]
[null,"F",{"$f":"'Input OD450 data'!C127","v":0},{"$f":"'Input OD450 data'!D127","v":0},{"$f":"'Input OD450 data'!E127","v":0}]
[null,"G",{"$f":"'Input OD450 data'!C128","v":0},{"$f":"'Input OD450 data'!D128","v":0},{"$f":"'Input OD450 data'!E128","v":0}]
[null,"H",{"$f":"'Input OD450 data'!C129","v":0},{"$f":"'Input OD450 data'!D129","v":0},{"$f":"'Input OD450 data'!E129","v":0}]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":0}]
[]
["Blank correction"]
[null,null,1,2,3]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":0},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":0}]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":0},0,0]
[null,"C",0,0,0]
[null,"D",0,0,0]
[null,"E",0,0,0]
[null,"F",0,0,0]
[null,"G",0,0,0]
[null,"H",0,0,0]
[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":0},0,{"$f":"AVERAGE(C88:D88)","v":0},{"$f":"STDEV(C88:D88)","v":0}]
[null,null,0,0,0,0]
[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[null,null,0,0,0,0]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,{"$f":"B7","v":100},{"$f":"E88","v":0},{"$f":"((J$102-M$102)/(1+((C101/L$102)^K$102))+M$102)","v":1},{"$f":"D101-E101","v":-1},{"$f":"F101^2","v":1},null,"Curve fit","A","B","C","D"]
[null,null,{"$f":"C101/B$6","v":40},0,1,{"$f":"D102-E102","v":-1},{"$f":"F102^2","v":1},null,"MsExcel Solver",1,1,1,1]
[null,null,16,0,1,-1,1]
[null,null,6.4,0,1,-1,{"$f":"F104^2","v":1}]
[null,null,2.56,0,1,-1,1,null,"Use Solver function to minimize SSR "]
[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
[null,null,0.16384000000000001,0,1,-1,1,null,{"$f":"SUM(G101:G108)","v":8}]

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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Dilution Increment",2.5]
["Initial Dilution",100]
[]
[]
["Sample ID"]
[null,null,1,2,3,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4"]
[null,"E","NC dil5","PC dil5","PC dil5","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6"]
[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
[]
["Dilutions"]
[null,null,1,2,3]
[null,"A",1,{"$f":"B7","v":100},{"$f":"B7","v":100}]
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["OD450"]
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[]
[]
[]
["StdCurve","MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
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[]
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[]
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[]
[]
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["StdCurve Fitting Results"]
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[null,null,0,0,0,0,null,null,"Click on SOLVER BUTTON here:"]
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[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
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[null,null,1.024,0,1,-1,1]
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[]
["Sample ID"]
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[null,"G","BLK","PC dil7","PC dil7","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8"]
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["Dilutions"]
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[]
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["StdCurve","MsExcel Std Curve"]
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[]
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[]
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[]
[]
[]
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[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
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[null,null,1.024,0,1,-1,1]
[null,null,0.40960000000000002,0,1,-1,1,null,"SSR: ∑(y-y pred)^2"]
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["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
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[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
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["OD450"]
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[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,46,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,47,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,48,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,49,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,50,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,51,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,52,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,53,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,54,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 7","index":15,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,56,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,57,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,58,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 8","index":16,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,64,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,65,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,66,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,67,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,68,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,69,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,70,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,71,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,72,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 9","index":17,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 10","index":18,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,82,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,83,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,84,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,85,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,86,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,87,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,88,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,89,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,90,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 11","index":19,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,92,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,93,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,94,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 12","index":20,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,100,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,101,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,102,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,103,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,104,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,105,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,106,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,107,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,108,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 13","index":21,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 14","index":22,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,118,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,119,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,120,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,121,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,122,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,123,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,124,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,125,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,126,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 15","index":23,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
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[null,null,128,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,129,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,130,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 16","index":24,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,136,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,137,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,138,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,139,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,140,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,141,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,142,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,143,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,144,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 17","index":25,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 18","index":26,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
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[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,154,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,155,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,156,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,157,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,158,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,159,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,160,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,161,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,162,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 19","index":27,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,165,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,166,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 20","index":28,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,172,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,173,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,174,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,175,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,176,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,177,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,178,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,179,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,180,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 21","index":29,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 22","index":30,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,190,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,191,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,192,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,193,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,194,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,195,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,196,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,197,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,198,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 23","index":31,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,201,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 24","index":32,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,208,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,209,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,210,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,211,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,212,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,213,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,214,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,215,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,216,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 25","index":33,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 26","index":34,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,226,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,227,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,228,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,229,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,230,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,231,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,232,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,233,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,234,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 27","index":35,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,237,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,238,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 28","index":36,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,244,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,245,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,246,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,247,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,248,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,249,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,250,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,251,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,252,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 29","index":37,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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{"sheet":"Step2_Curvefit_plate 30","index":38,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
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[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
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[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
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[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
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[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,262,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,263,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,264,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
[null,null,265,"SPL4","A7",{"$f":"I23","v":1},{"$f":"I34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E7",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F7",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G7",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H7",128,{"$error":"#REF!"},"","",""]
[null,null,266,"SPL5","A8",{"$f":"J23","v":1},{"$f":"J34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B8",{"$f":"J24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F8",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G8",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H8",128,{"$error":"#REF!"},"","",""]
[null,null,267,"SPL6","A9",{"$f":"K23","v":1},{"$f":"K34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B9",{"$f":"K24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E9",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G9",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
[null,null,268,"SPL7","A10",{"$f":"L23","v":1},{"$f":"L34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D10",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E10",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F10",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G10",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
[null,null,269,"SPL8","A11",{"$f":"M23","v":1},{"$f":"M34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B11",{"$f":"M24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D11",8,{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G223),G223-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H223>J$124,H223<M$124),((((J$124-M$124)/(H223-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I223),I223*F223,\"\")","v":""}]
[null,null,null,null,"E11",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F11",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G11",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H11",128,{"$error":"#REF!"},"","",""]
[null,null,270,"SPL9","A12",{"$f":"N23","v":1},{"$f":"N34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B12",{"$f":"N24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C12",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D12",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E12",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F12",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 31","index":39,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
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[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
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[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
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[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
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[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
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[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
[null,null,273,"SPL3","A6",{"$f":"H23","v":1},{"$f":"H34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B6",{"$f":"H24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C6",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D6",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E6",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F6",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G6",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H6",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B7",{"$f":"I24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C7",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D7",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C8",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D8",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E8",16,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C9",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D9",8,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"F9",32,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H9",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"B10",{"$f":"L24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C10",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"H10",128,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"C11",4,{"$error":"#REF!"},"","",""]
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[null,null,null,null,"G12",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H12",128,{"$error":"#REF!"},"",{"$f":"IF(AND(H235>J$124,H235<M$124),((((J$124-M$124)/(H235-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I235),I235*F235,\"\")","v":""}]

{"sheet":"Step2_Curvefit_plate 32","index":40,"n_rows":235,"n_cols":17,"rows_kept":235,"cut":false,"hidden":true,"merged":[[0,0,0,14],[126,8,126,12]]}
["PSPT ELISA 4PL curve fit and data transformation"]
["Technician #"]
["Technician #"]
["Date of assay"]
["Plate Number"]
["Reader Type"]
["Software Version"]
[]
[]
["Sample ID"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12,null,"PC","= Positive control (or reference serum)"]
[null,"A","NC dil1","PC dil1","PC dil1","SPL1 dil1","SPL2 dil1","SPL3 dil1","SPL4 dil1","SPL5 dil1","SPL6 dil1","SPL7 dil1","SPL8 dil1","SPL9 dil1","Well ID",null,"Titer is arbitrarily set to 100 EU/mL "]
[null,"B","NC dil2","PC dil2","PC dil2","SPL1 dil2","SPL2 dil2","SPL3 dil2","SPL4 dil2","SPL5 dil2","SPL6 dil2","SPL7 dil2","SPL8 dil2","SPL9 dil2","Well ID"]
[null,"C","NC dil3","PC dil3","PC dil3","SPL1 dil3","SPL2 dil3","SPL3 dil3","SPL4 dil3","SPL5 dil3","SPL6 dil3","SPL7 dil3","SPL8 dil3","SPL9 dil3","Well ID","SPL sera","= test sera from vaccinated mouse"]
[null,"D","NC dil4","PC dil4","PC dil4","SPL1 dil4","SPL2 dil4","SPL3 dil4","SPL4 dil4","SPL5 dil4","SPL6 dil4","SPL7 dil4","SPL8 dil4","SPL9 dil4","Well ID"]
[null,"E","NC dil5","PC dil5","PC dil5","SPL1 dil5","SPL2 dil5","SPL3 dil5","SPL4 dil5","SPL5 dil5","SPL6 dil5","SPL7 dil5","SPL8 dil5","SPL9 dil5","Well ID","NC","=naïve mouse serum"]
[null,"F","NC dil6","PC dil6","PC dil6","SPL1 dil6","SPL2 dil6","SPL3 dil6","SPL4 dil6","SPL5 dil6","SPL6 dil6","SPL7 dil6","SPL8 dil6","SPL9 dil6","Well ID"]
[null,"G","BLK","PC dil7","PC dil7","SPL1 dil7","SPL2 dil7","SPL3 dil7","SPL4 dil7","SPL5 dil7","SPL6 dil7","SPL7 dil7","SPL8 dil7","SPL9 dil7","Well ID","BLK","= assay diluent "]
[null,"H","BLK","PC dil8","PC dil8","SPL1 dil8","SPL2 dil8","SPL3 dil8","SPL4 dil8","SPL5 dil8","SPL6 dil8","SPL7 dil8","SPL8 dil8","SPL9 dil8","Well ID"]
[]
["Dilutions"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",1,100,100,1,1,1,1,1,1,1,1,1]
[null,"B",2,50,50,2,2,2,2,2,2,2,2,2]
[null,"C",4,25,25,4,4,4,4,4,4,4,4,4]
[null,"D",8,12.5,12.5,8,8,8,8,8,8,8,8,8]
[null,"E",16,6.25,6.25,16,16,16,16,16,16,16,16,16]
[null,"F",32,3.125,3.125,32,32,32,32,32,32,32,32,32]
[null,"G",null,1.5625,1.5625,64,64,64,64,64,64,64,64,64]
[null,"H",null,0.78125,0.78125,128,128,128,128,128,128,128,128,128]
[]
["OD450"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"B",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"C",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"D",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"E",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"F",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"G",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"H",{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},{"$f":"'Input OD450 data'!#REF!","v":{"$error":"#REF!"}},450]
[null,"avg BLK",{"$f":"AVERAGE(C40:C41)","v":{"$error":"#REF!"}}]
[]
["Blank correction"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"IF(ISNUMBER(C34),C34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(D34),D34-$C$42,\"\")","v":""},{"$f":"IF(ISNUMBER(E34),E34-$C$42,\"\")","v":""},"","","","","","","","","","Blank 450"]
[null,"B",{"$f":"IF(ISNUMBER(C35),C35-$C$42,\"\")","v":""},"","","","","","","","","","","","Blank 450"]
[null,"C","","","","","","","","","","","","","Blank 450"]
[null,"D","","","","","","","","","","","","","Blank 450"]
[null,"E","","","","","","","","","","","","","Blank 450"]
[null,"F","","","","","","","","","","","","","Blank 450"]
[null,"G","","","","","","","","","","","","","Blank 450"]
[null,"H","","","","","","","","","","","","","Blank 450"]
[]
["Concentration"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"I158","v":""},{"$f":"I139","v":{"$error":"#VALUE!"}},{"$f":"I140","v":{"$error":"#VALUE!"}},{"$f":"I164","v":""},{"$f":"I172","v":""},{"$f":"I180","v":""},{"$f":"I188","v":""},{"$f":"I196","v":""},{"$f":"I204","v":""},{"$f":"I212","v":""},{"$f":"I220","v":""},{"$f":"I228","v":""},"[Conc]"]
[null,"B","",{"$f":"I141","v":{"$error":"#VALUE!"}},{"$f":"I142","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"C","",{"$f":"I143","v":{"$error":"#VALUE!"}},{"$f":"I144","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"D","",{"$f":"I145","v":{"$error":"#VALUE!"}},{"$f":"I146","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"E","",{"$f":"I147","v":{"$error":"#VALUE!"}},{"$f":"I148","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"F","",{"$f":"I149","v":{"$error":"#VALUE!"}},{"$f":"I150","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"G","",{"$f":"I151","v":{"$error":"#VALUE!"}},{"$f":"I152","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[null,"H","",{"$f":"I153","v":{"$error":"#VALUE!"}},{"$f":"I154","v":{"$error":"#VALUE!"}},"","","","","","","","","","[Conc]"]
[]
[]
["Concentration x dilution"]
[null,null,1,2,3,4,5,6,7,8,9,10,11,12]
[null,"A",{"$f":"J158","v":""},null,null,{"$f":"J164","v":""},{"$f":"J172","v":""},{"$f":"J180","v":""},{"$f":"J188","v":""},{"$f":"J196","v":""},{"$f":"J204","v":""},{"$f":"J212","v":""},{"$f":"J220","v":""},{"$f":"J228","v":""},"[Conc] x Dil"]
[null,"B",{"$f":"J159","v":""},null,null,{"$f":"J165","v":""},{"$f":"J173","v":""},{"$f":"J181","v":""},{"$f":"J189","v":""},{"$f":"J197","v":""},{"$f":"J205","v":""},{"$f":"J213","v":""},{"$f":"J221","v":""},{"$f":"J229","v":""},"[Conc] x Dil"]
[null,"C","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"D","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"E","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"F","",null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"G",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[null,"H",null,null,null,"","","","","","","","","","[Conc] x Dil"]
[]
[]
["StdCurve","ELISA Reader software Std Curve",null,null,null,null,null,null,null,null,null,"MsExcel Std Curve"]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
[]
["StdCurve Fitting Results"]
[null,null,"Y",null,"avg","stdev"]
[null,null,{"$f":"D46","v":""},"",{"$f":"AVERAGE(C110:D110)","v":{"$error":"#DIV/0!"}},{"$f":"STDEV(C110:D110)","v":{"$error":"#DIV/0!"}}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,null,"Click on SOLVER BUTTON here:"]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,"","",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[]
[]
[null,null,"Use 4PL formula:",null,null,null,null,null,"Fitting constants"]
[null,null,"Y = (A-D)/(1+(X/C)^B) + D"]
[null,null,"X","Y","Y_Best fit","residual","residual^2",null,null,"Min asymptote","slope","Intercept","Max asymptote"]
[null,null,100,{"$f":"E110","v":{"$error":"#DIV/0!"}},{"$f":"((J$124-M$124)/(1+((C123/L$124)^K$124))+M$124)","v":1},{"$f":"D123-E123","v":{"$error":"#DIV/0!"}},{"$f":"F123^2","v":{"$error":"#DIV/0!"}},null,"Curve fit","A","B","C","D"]
[null,null,50,{"$error":"#DIV/0!"},1,{"$f":"D124-E124","v":{"$error":"#DIV/0!"}},{"$f":"F124^2","v":{"$error":"#DIV/0!"}},null,"MsExcel Solver",1,1,1,1]
[null,null,25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Lab software"]
[null,null,12.5,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$f":"F126^2","v":{"$error":"#DIV/0!"}}]
[null,null,6.25,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"Use Solver function to minimize SSR "]
[null,null,3.125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"}]
[null,null,1.5625,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,"SSR: ∑(y-y pred)^2"]
[null,null,0.78125,{"$error":"#DIV/0!"},1,{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},null,{"$f":"SUM(G123:G130)","v":{"$error":"#DIV/0!"}}]
[]
[]
[null,null,"Interpolate Y data using formula",null,null,null,null,null,null,null,null,null,null," "]
[null,null,"Inv function"]
[null,null,"(((((A - D) / (y - D)) - 1) ^ (1 / B)) * C)"]
[]
["Stacked data",null,"Mouse#","Well ID","Well","Conc/Dil","OD450nm","OD450 - BLK","Interpolated [Conc]","[Conc] x Dil"]
[]
[null,null,null,"PC1","A2",100,{"$f":"D34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G139),G139-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"((((J$124-M$124)/(H139-M$124))-1)^(1/K$124))*L$124","v":{"$error":"#VALUE!"}}]
[null,null,null,null,"A3",100,{"$f":"E34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G140),G140-AVERAGE($G$155:$G$156),\"\")","v":""},{"$error":"#VALUE!"}]
[null,null,null,"PC2","B2",50,{"$f":"D35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"B3",50,{"$f":"E35","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC3","C2",25,{"$f":"D36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"C3",25,{"$f":"E36","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC4","D2",12.5,{"$f":"D37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"D3",12.5,{"$f":"E37","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC5","E2",6.25,{"$f":"D38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"E3",6.25,{"$f":"E38","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC6","F2",3.125,{"$f":"D39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"F3",3.125,{"$f":"E39","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC7","G2",1.5625,{"$f":"D40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"G3",1.5625,{"$f":"E40","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"PC8","H2",0.78125,{"$f":"D41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,null,"H3",0.78125,{"$f":"E41","v":{"$error":"#REF!"}},"",{"$error":"#VALUE!"}]
[null,null,null,"BLK","G1",null,{"$f":"C40","v":{"$error":"#REF!"}}]
[null,null,null,null,"H1",null,{"$f":"C41","v":{"$error":"#REF!"}}]
[]
[null,null,null,"NC","A1",{"$f":"C23","v":1},{"$f":"C34","v":{"$error":"#REF!"}},{"$f":"IF(ISNUMBER(G158),G158-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H158>J$124,H158<M$124),((((J$124-M$124)/(H158-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I158),I158*F158,\"\")","v":""}]
[null,null,null,null,"B1",{"$f":"C24","v":2},{"$error":"#REF!"},{"$f":"IF(ISNUMBER(G159),G159-AVERAGE($G$155:$G$156),\"\")","v":""},{"$f":"IF(AND(H159>J$124,H159<M$124),((((J$124-M$124)/(H159-M$124))-1)^(1/K$124))*L$124,\"\")","v":""},{"$f":"IF(ISNUMBER(I159),I159*F159,\"\")","v":""}]
[null,null,null,null,"C1",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D1",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E1",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F1",32,{"$error":"#REF!"},"","",""]
[null,null,280,"SPL1","A4",{"$f":"F23","v":1},{"$f":"F34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B4",{"$f":"F24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C4",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D4",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E4",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F4",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G4",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H4",128,{"$error":"#REF!"},"","",""]
[null,null,281,"SPL2","A5",{"$f":"G23","v":1},{"$f":"G34","v":{"$error":"#REF!"}},"","",""]
[null,null,null,null,"B5",{"$f":"G24","v":2},{"$error":"#REF!"},"","",""]
[null,null,null,null,"C5",4,{"$error":"#REF!"},"","",""]
[null,null,null,null,"D5",8,{"$error":"#REF!"},"","",""]
[null,null,null,null,"E5",16,{"$error":"#REF!"},"","",""]
[null,null,null,null,"F5",32,{"$error":"#REF!"},"","",""]
[null,null,null,null,"G5",64,{"$error":"#REF!"},"","",""]
[null,null,null,null,"H5",128,{"$error":"#REF!"},"","",""]
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